Human serum albumin interactions with C60 fullerene studied by spectroscopy, small-angle neutron scattering, and molecular dynamics simulations
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- 作者：Song Li (1)
Xiongce Zhao (2) (6)
Yiming Mo (3) (4) (7)
Peter T. Cummings (1) (2)
William T. Heller (3) (4) (5) - 关键词：Fullerene ; Serum albumin ; Nanotoxicity ; Small ; angle neutron scattering ; Molecular dynamics simulation
- 刊名：Journal of Nanoparticle Research
- 出版年：2013
- 出版时间：July 2013
- 年：2013
- 卷：15
- 期：7
- 全文大小：516KB
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Xiongce Zhao (2) (6)
Yiming Mo (3) (4) (7)
Peter T. Cummings (1) (2)
William T. Heller (3) (4) (5)
1. Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, 37235, USA
2. Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA
6. NIDDK, National Institutes of Health, Bethesda, MD, 20892, USA
3. Center for Structural Molecular Biology, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA
4. Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA
7. Institute of Agriculture, University of Tennessee, 109 McCord Hall, Knoxville, TN, 37996, USA
5. Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA - ISSN：1572-896X
文摘
Concern about the toxicity of engineered nanoparticles, such as the prototypical nanomaterial C60 fullerene, continues to grow. While, evidence continues to mount that C60 and its derivatives may pose health hazards, the specific molecular interactions of these particles with biological macromolecules require further investigation. In this article, we report combined experimental and theoretical studies on the interaction of one of the most prevalent proteins in the human body, human serum albumin (HSA), with C60 in an aqueous environment. The C60–HSA interaction was probed by circular dichroism (CD) spectroscopy, small-angle neutron scattering (SANS), and atomistic molecular dynamics (MD) simulations to understand C60-driven changes in the structure of HSA in solution. The CD spectroscopy demonstrates that the secondary structure of the protein decreases in α-helical content in response to the presence of C60 (0.68?nm in diameter). Similarly, C60 produces subtle changes in the solution conformation of HSA (an 8.0?nm?×?3.8?nm protein), as evidenced by the SANS data and MD simulations, but the data do not indicate that C60 changes the oligomerization state of the protein, such as by inducing aggregation. The results demonstrate that the interaction is not highly disruptive to the protein in a manner that would prevent it from performing its physiological function.