Pseudovibrio hongkongensis sp. nov., isolated from a marine flatworm

详细信息    查看全文

• 作者：Ying Xu ; Qi Li ; Renmao Tian ; Qiliang Lai ; Yu Zhang
• 关键词：Pseudovibrio hongkongensis sp. nov. ; Flatworm ; Taxonomy
• 刊名：Antonie van Leeuwenhoek
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：108
• 期：1
• 页码：127-132
• 全文大小：401 KB
• 参考文献：Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman J, Smith JA, Struhl K (2002) Short protocols in molecular biology: a compendium of methods from current protocols in molecular biology. Wiley, Hoboken
  Dong X, Cai M (2001) Determinative manual for routine bacteriology. Scientific Press (English translation), Beijing
  Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368-76PubMed View Article
  Fukunaga Y, Kurahashi M, Tanaka K, Yanagi K, Yokota A, Harayama S (2006) Pseudovibrio ascidiaceicola sp. nov., isolated from ascidians (sea squirts). Int J Syst Evol Microbiol 56:343-47PubMed View Article
  Hosoya S, Yokota A (2007) Pseudovibrio japonicas sp. nov., isolated from coastal seawater in Japan. Int J Syst Evol Microbiol 57:1952-955PubMed View Article
  Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716-21PubMed View Article
  Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acids techniques in bacterial systematics. Wiley, Chichester, pp 115-75
  Mesbah M, Whitman WB (1989) Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479:297-06PubMed View Article
  O’Halloran JA, Barbosa TM, Morrissey JP, Kennedy J, Dobson AD, O’Gara F (2014) Pseudovibrio axinellae sp. nov., isolated from an Irish marine sponge. Int J Syst Envol Microbiol 63:141-45View Article
  Rzhetsky A, Nei M (1992) Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35:367-75PubMed View Article
  Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406-25PubMed
  Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16
  Shieh WY, Lin Y-T, Jean WD (2004) Pseudovibrio denitrificans gen. nov., sp. nov., a marine, facultatively anaerobic, fermentative bacterium capable of denitrification. Int J Syst Evol Microbiol 54:2307-312PubMed View Article
  Skerman V (1967) A guide to the identification of the genera of bacteria: with methods and digests of generic characteristics. Williams & Wilkins, Baltimore
  Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731-739PubMed Central PubMed View Article
  
• 作者单位：Ying Xu (1) (2)
  Qi Li (2)
  Renmao Tian (2)
  Qiliang Lai (3)
  Yu Zhang (1)
  
  1. Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, China
  2. Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China
  3. Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Xiamen, 361005, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Soil Science and Conservation
  
• 出版者：Springer Netherlands
• ISSN：1572-9699

文摘

A Gram-negative, motile, rod-shaped bacterial strain, designated UST20140214-015BT, was isolated from a marine flatworm (Polyclad). The bacterium was found to be Gram-negative, oxidase and catalase positive, long-rod shaped, and motile by means of several peritrichous or subpolar flagella. Strain UST20140214-015BT grew optimally at 28?°C, at pH 7.0 and in the presence of 3?% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain UST20140214-015BT belongs to the genus Pseudovibrio, with highest sequence similarity to Pseudovibrio japonicus WSF2T (96.9?%), followed by P. ascidiaceicola F423T (96.7?%), P. denitrificans DN34T (96.6?%), P. axinellae Ad2T (96.3?%). All the other species shared <95.5?% sequence similarity. The principal fatty acids were identified as summed feature 8 (C_18:1 ω7c/ω6c, as defined by the MIDI system; 70.8?%), C_16:0 (9.1?%), summed feature 3 (C_16:1 ω6c/C_16:1 ω7c; 5.7?%). The G+C content of the chromosomal DNA was determined to be 53.3?mol%. The combined genotypic and phenotypic data show that strain UST20140214-015BT represents a novel species within the genus Pseudovibrio, for which the name Pseudovibrio hongkongensis sp. nov. is proposed, with the type strain UST20140214-015BT (=KCTC 42383T?=?MCCC 1K00451T).