Target expression of Staphylococcus enterotoxin A from an oncolytic adenovirus suppresses mouse bladder tumor growth and recruits CD3+ T cell

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• 作者：Conghui Han (1)
  Lin Hao (1)
  Meng Chen (1)
  Jianpeng Hu (1)
  Zhenduo Shi (1)
  Zhiguo Zhang (1)
  Bingzheng Dong (1)
  Yu Fu (1)
  Changsong Pei (2)
  Yongping Wu (3)
  
• 关键词：Adenovirus ; Bladder cancer ; SEA ; hTERT ; HRE ; Oncolytic ; CD3+ T cell
• 刊名：Tumor Biology
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：34
• 期：5
• 页码：2863-2869
• 全文大小：
• 作者单位：Conghui Han (1)
  Lin Hao (1)
  Meng Chen (1)
  Jianpeng Hu (1)
  Zhenduo Shi (1)
  Zhiguo Zhang (1)
  Bingzheng Dong (1)
  Yu Fu (1)
  Changsong Pei (2)
  Yongping Wu (3)
  
  1. Department of Urology, The Affiliated School of Clinical Medicine of Xuzhou Medical College, Xuzhou Central Hospital, 199 Jie Fang Nan Road, Xuzhou, 221009, People? Republic of China
  2. Department of Urology, The Second Affiliated Hospital, Xuzhou Medical College, 32 Mei Jian Road, Xuzhou, 221006, People? Republic of China
  3. Department of Pathology, Xuzhou Medical College, No. 84 West Huaihai Road, Xuzhou, 221002, China
  
• ISSN：1423-0380

文摘

We recently engineered an oncolytic adenovirus (PPE3-SEA) that expresses the superantigen, Staphylococcus enterotoxin A (SEA), and that has enhanced tumor specificity because the telomerase reverse transcriptase and hypoxia-inducible factor promoters regulate expression of E1A and E1B genes, respectively. Here, we evaluated the PPE3-SEA adenovirus anti-tumor activity against MB49 mouse bladder cancer cell proliferation in vitro and in vivo. PPE3-SEA infection of murine MB49 cells in vitro induced cytopathic effects, and significant expression of SEA mRNA and protein, as measured by RT-PCR and western blot, respectively. Subcutaneous MB29 bladder tumors were established in syngeneic C57BL/6 mice. After 10?days, tumors were injected with either oncolytic virus or PBS. Tumor dimensions were measured on days?1, 3, 5, 7, 9, and 11 post-treatment and tumor volumes were calculated. One of eight PPE3-SEA-treated mice had no tumor by day?9. PPE3-SEA?treated group had significantly lower mean tumor volume beginning on day?5 post-treatment (p?<?0.01), more fibrous tissue in the tumor, and increased presence of infiltrating CD3+ T cells than those of the control group. Gross appearance and histologic sections from the livers and kidneys of the PPE3-SEA-treated group were similar to those of the control group. In conclusion, oncolytic adenoviruses can provide a novel delivery vehicle for SEA to tumor sites, and PPE3-SEA warrants further study as a potential anti-tumor agent for bladder cancer.