Epieriocalyxin A Induces Cell Apoptosis Through JNK and ERK1/2 Signaling Pathways in Colon Cancer Cells

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• 作者：Zhou Wang ; Zhijie Xu ; Zhengchuan Niu ; Benjia Liang…
• 关键词：Epieriocalyxin A ; Colorectal cancer ; Cell apoptosis ; Signaling pathway
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：73
• 期：2
• 页码：559-564
• 全文大小：1,690 KB
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• 作者单位：Zhou Wang (1) (2)
  Zhijie Xu (3)
  Zhengchuan Niu (1) (2)
  Benjia Liang (1) (2)
  Jun Niu (1)
  
  1. Department of Hepatobiliary Surgery, Qilu Hospital, Shandong University, Jinan, 250012, Shandong, People鈥檚 Republic of China
  2. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Public Health, Jinan, 250012, Shandong, People鈥檚 Republic of China
  3. Department of Anesthesiology, Qilu Hospital, Shandong University, Jinan, 250012, Shandong, People鈥檚 Republic of China
  
• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Biotechnology; Cell Biology; Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1559-0283

文摘

Colorectal cancer is one of the most commonly diagnosed cancers in the world. Currently, drug resistance of cancer cell to chemotherapy is a major cause for cancer recurrence and death of the patients; therefore, new therapeutic strategy is required to improve the care of colorectal cancer patients. The Chinese herb, Isodon eriocalyx, has been used a therapeutic for a long time in China. In this study, we showed that Epieriocalyxin A (EpiA), a diterpenoid isolated from I. eriocalyx, suppressed Caco-2 colon cancer cell growth. EpiA induced annexin V flipping in cell membrane and DNA fragment. We also showed that EpiA induced the generation of ROS in cells, as well as damage of the mitochondrial membrane. Western blot results showed that both JNK and ERK1/2 activation was decreased after EpiA treatment in a dose-dependent manner. EpiA increased the expression of caspase 3 and Bax, and decreased Bcl2 expression. Our results suggest that EpiA is a novel compound that induces colon cancer apoptosis. EpiA could be a potential drug for colon cancer therapy in the future. Keywords Epieriocalyxin A Colorectal cancer Cell apoptosis Signaling pathway