OsCPK20 positively regulates Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea

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• 作者：Liwen Fu (1)
  Xiangchun Yu (1)
  Chengcai An (1)
  
• 关键词：CDPK ; Plant immunity ; Salicylic acid ; Jasmonic acid
• 刊名：Acta Physiologiae Plantarum
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：36
• 期：2
• 页码：273-282
• 全文大小：743 KB
• 作者单位：Liwen Fu (1)
  Xiangchun Yu (1)
  Chengcai An (1)
  
  1. State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China
  
• ISSN：1861-1664

文摘

Calcium-dependent protein kinases are important decoders of calcium signals in plants, which are involved in plant immunity. We report isolation and functional characterization of a pathogen-responsive OsCPK20 gene in rice. The expression of OsCPK20 in rice was significantly induced following treatment with a Magnaporthe grisea elicitor. Overexpression of constitutively active OsCPK20 in Arabidopsis enhanced the resistance to infection with Pseudomonas syringae pv. tomato, associated with elevated expression of both SA- and JA-related defense genes. Similarly, transgenic rice plants containing constitutively active OsCPK20 exhibited enhanced resistance to blast fungus M. grisea. The enhanced resistance in the transgenic Arabidopsis and rice was associated with activated expression of both SA- and JA-related defense genes. We also found that OsCPK20 was significantly induced by drought stress, indicating that OsCPK20 might be involved in plant response to drought stress. Taken together, our results indicate that rice OsCPK20 positively regulates Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against M. grisea, and that it may enhance disease resistance by activating both SA- and JA-dependent defense responses.