A new enzyme-linked immunosorbent assay (ELISA) for human free and bound kallikrein 9
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- 作者：Panagiota Filippou ; Dimitrios Korbakis ; Sofia Farkona…
- 关键词：Kallikreins ; Kallikrein 9 ; ELISA ; Immunocapture ; PRM ; Serine protease inhibitors ; a1 ; Antichymotrypsin ; Hybrid assays
- 刊名：Clinical Proteomics
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：14
- 期：1
- 全文大小：1157KB
- 刊物主题：Cell Biology; Pathology; Biotechnology; Proteomics;
- 出版者：BioMed Central
- ISSN：1559-0275
- 卷排序：14
文摘
BackgroundKallikrein 9 (KLK9) is a member of the human kallikrein-related peptidases family, whose physiological role and implications in disease processes remain unclear. The active form of the enzyme is predicted to have chymotryptic activity. In the present study, we produced for the first time the active recombinant protein and monoclonal antibodies, and developed novel immunoassays for the quantification of free and bound KLK9 in biological samples.