DNA fingerprinting of oil camellia cultivars with SSR markers
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- 作者：Yingnan Chen ; Xiaogang Dai ; Jing Hou ; Hongwei Guan ; Yuxiao Wang…
- 关键词：Oil camellia ; Elite cultivar ; DNA fingerprinting ; Microsatellite
- 刊名：Tree Genetics & Genomes
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：12
- 期：1
- 全文大小：801 KB
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Xiaogang Dai (1)
Jing Hou (1)
Hongwei Guan (1)
Yuxiao Wang (1)
Yi Li (2)
Tongming Yin (1)
1. The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, 210037, China
2. Department of Plant Science and Landscape architecture, University of Connecticut, Storrs, CT, 06269, USA - 刊物主题：Forestry; Plant Genetics & Genomics; Plant Breeding/Biotechnology; Tree Biology; Biotechnology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1614-2950
文摘
Oil camellia (Camellia oleifera Abel.) is a widely cultivated economically important plant. In China, more than 100 elite cultivars have been authorized for commercial plantations, representing the developmental base for the oil camellia industry. Since oil camellia seedlings are frequently traded and introduced to new places, there is an active demand to develop a rapid and reliable identification toolkit to protect and ensure the effective use of these resources. In this study, ten highly polymorphic simple sequence repeat (SSR) primer pairs were screened from the SSR primer database of oil camellia, and DNA fingerprints of 128 elite oil camellia cultivars were constructed with these primer pairs. By analyzing the fingerprinting profiles, all of these cultivars were found to have unique DNA fingerprints, except ‘Changlin 40’ and ‘Fuyang 40’, which shared a complete similarity across all of the fingerprints generated by these ten SSR primer pairs, indicating that they were either synonyms or vegetative mutants. Synonyms are a major source of confusion in protecting and managing new varieties of plants. Although the synonymy of these two cultivars needs to be further confirmed, our data indicated a high possibility of synonyms among the authorized oil camellia cultivars. This study provides a desirable genetic toolkit for the identification and management of the elite oil camellia cultivars, and also for the intellectual property right protection of breeders. Keywords Oil camellia Elite cultivar DNA fingerprinting Microsatellite