Analysis of methylation-sensitive amplified polymorphism in different cotton accessions under salt stress based on capillary electrophoresis

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• 作者：Baohua Wang ; Rong Fu ; Mi Zhang ; Zhenqian Ding ; Lei Chang ; Xinyu Zhu&#8230
• 关键词：Cotton ; Salt stress ; Capillary electrophoresis ; Methylation ; sensitive amplification polymorphism
• 刊名：Genes & Genomics
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：37
• 期：8
• 页码：713-724
• 全文大小：971 KB
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• 作者单位：Baohua Wang (1)
  Rong Fu (1)
  Mi Zhang (1)
  Zhenqian Ding (3)
  Lei Chang (1)
  Xinyu Zhu (1)
  Yafeng Wang (1)
  Baoxiang Fan (2)
  Wuwei Ye (2)
  Youlu Yuan (2)
  
  1. School of Life Sciences, Nantong University, Nantong, 226019, Jiangsu, People鈥檚 Republic of China
  3. Xuzhou Institute of Agricultural Sciences of Xu-huai Region of Jiangsu, Xuzhou, 221131, Jiangsu, People鈥檚 Republic of China
  2. State Key Laboratory of Cotton Biology; Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, People鈥檚 Republic of China
  
• 刊物主题：Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Human Genetics;
• 出版者：Springer Netherlands
• ISSN：2092-9293

文摘

A methylation-sensitive amplification polymorphism method based on capillary electrophoresis was used to analyze DNA methylation levels in three cotton accessions, two salt-tolerant accessions CCRI 35 and Zhong 07 and one salt-sensitive accession CCRI 12. Many categories of DNA methylation happened in the three cotton accessions under salt treatment, including hypermethylation, hypomethylation, and other patterns. Hypermethylation happened at a significantly higher rate than that of hypomethylation in salt-tolerant accessions CCRI 35 and Zhong 07. On the contrary, in salt-sensitive accession CCRI 12, hypomethylation happened at a significantly higher rate than that of hypermethylation. In general, the global DNA methylation level significantly increased under salt stress in both salt-tolerant accessions CCRI 35 and Zhong 07, whereas there was no significant difference in the salt-sensitive accessions CCRI 12. Our results suggested that salt-tolerant cotton might have a mechanism of increasing the methylation level when responding to salt stress; the increase of the global level of DNA methylation and also different methylation patterns might play important roles in tolerance to salt stress in cotton. Some interesting genes were found through cloning and analysis of differently methylated DNA sequences, which might contribute to salt tolerance in cotton.