Actinidin, a protease from kiwifruit, induces changes in morphology and adhesion of T84 intestinal epithelial cells
- 作者：Milena 膶avi膰a ; Milica Grozdanovi膰b ; Aleksandar Baji膰c ; Tatjana Srdi膰-Raji膰a ; Pavle R. An膽jusc ; Marija Gavrovi膰-Jankulovi膰b ; mgavrov@chem.bg.ac.rs
- 关键词：Actinidia sp. ; Kiwifruit ; Actinidin ; T84 cells ; Intestinal disorders
- 刊名：Phytochemistry
- 出版年：2012
- 期刊代码：85_00319422
- 类别：ch
- 出版时间：May, 2012
- 卷：77
- 期：Complete
- 页码：46-52
- 文件大小：609 K
摘要
Actinidin belongs to the papain-like family of cysteine proteases and is a major kiwifruit allergen. In this study, the effect of actinidin on cellular morphology and adhesion of T84 intestinal cells was investigated. Both rounding and detachment of T84 cells were observed upon actinidin treatment. The morphological changes and cell desquamation was protease-dependent, as well as time- and concentration-dependent. Changes of intercellular adhesion and adhesion of epithelial cells to collagen upon actinidin treatment could be responsible for the cell rounding and give rise to discontinuous breaches in the epithelial monolayer observed in this study. Actinidin鈥檚 action on cell morphology, adhesion and monolayer integrity were not due to compromised viability of T84 epithelial cells, as confirmed by MTT assay and flow cytometric analysis of the cell cycle. Damage to the epithelial monolayer of the intestine induced by actinidin should be further evaluated as an important factor in the development of kiwifruit allergy and other intestinal disorders.