Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide

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• 作者：Bing Liu¹ ; James A. Garnett¹ ; Wei-chao Lee ; Jing Lin ; Paula Salgado ; Jonathan Taylor ; Yingqi Xu ; Sebastian Lambert ; Ernesto Cota ; Steve Matthews ; ^{s.j.matthews@imperial.ac.uk}
• 关键词：Deuterium oxide ; Crystallography ; Additive ; Pilin
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：4 May, 2012
• 卷：421
• 期：2
• 页码：208-213
• 文件大小：899 K

摘要

The use of heavy water (D₂O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D₂O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.