Synthesis and cytotoxic properties of new fluorodeoxyglucose-coupled chlorambucil derivatives
- 作者：Bastien Reux ; Valé ; rie Weber ; Marie-Josephe Galmier ; Michè ; le Borel ; Michel Madesclaire ; Jean-Claude Madelmont ; Eric Debiton ; Pascal Coudert
- 关键词：Chlorambucil ; Antitumor agents ; 2-Fluoro-2-deoxy-d-glucose derivatives ; Drug carrier
- 刊名：Bioorganic and Medicinal Chemistry
- 出版年：2008
- 期刊代码：9_09680896
- 类别：ch
- 出版时间：1 May 2008
- 卷：16
- 期：9
- 页码：5004-5020
- 文件大小：293 K
摘要
Frequently used in the treatment of malignant cells, alkylating agents, like most anticancer substances, produce adverse side effects caused by the toxicity of the agents toward normal tissues and lose efficiency through poor distribution to target sites. Our approach to developing more selective drugs with low systemic toxicity is based on the premise that the body distribution and cell uptake of a drug can be altered by attaching a neoplastic cell-specific uptake enhancer, such as 2-fluoro-2-deoxyglucose (FDG), the radiotracer most frequently used in PET for tumor imaging. Two properties of deoxyglucose, namely preferential accumulation in neoplastic cells and inhibition of glycolysis, underpin this targeting approach. Here, we report the synthesis of 19 new chlorambucil glycoconjugates in which the alkylating drug is attached to the C-1 position of FDG, directly or via different linkages. This set of compounds was evaluated for in vitro cytotoxicity against different human normal and tumor cell lines. There was a significant improvement in the in vitro cytotoxicity of peracetylated glucoconjugates compared with the free substance. Four compounds were finally selected for further in vivo studies owing to their lack of oxidative stress-inducing properties.