Experimental SSM-CVB3 infection in macaques

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• 作者：Tiesuo Han^a ; ¹ ; ^{hts-820228@163.com} ; Wenqi He^a ; ^{hewq@jlu.edu.cn} ; Deguang Song^a ; ¹ ; Kui Zhao^a ; ^{kuizhao@jlu.edu.cn} ; Chenchen Wu^a ; Feng Gao^a ; ^b ; Huijun Lu^b ; Xianying Gai^c ; Xinrui Wang^b ; Fei Li^a ; Cuicui Ji^a ; Xijuan Lin^a
• 关键词：Macaque model ; SSM-CVB3 ; Pathogenicity ; Hepatic damage ; Renal damage
• 刊名：Experimental and Molecular Pathology
• 出版年：2012
• 期刊代码：291_00144800
• 类别：med
• 出版时间：February, 2012
• 卷：92
• 期：1
• 页码：131-139
• 文件大小：2033 K

摘要

Objective

To evaluate the pathogenicity of SSM-CVB3 in a macaque model.

Methods

The clinical symptoms of macaques were recorded; hematological, biochemical and histopathological evaluations were completed; viral titers and neutralization titers (NT-titers) in sera were tested; and the mRNA levels of SSM-CVB3, coxsackievirus and adenovirus receptor (CAR) and decay accelerating factor (DAF) were determined.

Results

After SSM-CVB3 infection, the macaques showed a lack of activity, a poor appetite, a higher body temperature, and severe diarrhea. The macaques also developed hematuria and albuminuria at 4 to 10 days post-inoculation. Virus titers (5.1-6.5 LogTCID₅₀/mL) were higher at 6 to 10 days post-inoculation, and NT-titers (6.5-7.3 Log2) reached plateaus at 8 to 14 days post-inoculation. The infected macaques developed serious anemia with decreased RBC and WBC, but the percentages of LYM were increased. The levels of CK, CK-MB, AST and ALT in the sera were 84-169 U/L, 87.6-271.1 U/L, 43-87 U/L and 43-82 U/L, respectively, and all of those were higher than normal. Histological analysis showed obvious cardiac, hepatic and renal damages in the infected macaques and the mRNA contents of SSM-CVB3, CAR and DAF in the heart, liver and kidneys of infected macaques were higher (P < 0.05).

Conclusion

This was the first report on experimental SSM-CVB3 infections in macaques with serious hepatic and renal damage, except for myocarditis. The information obtained from this study suggests that the SSM-CVB3 strain and this macaque model could be used for studying CVB3-induced cardiac, hepatic or renal diseases.