Differential expression of Arabidopsis defense-related genes in response to sulfur dioxide

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• 作者：Lihong Li ; Huilan Yi ; ^{yihuilan@yahoo.com.cn}
• 关键词：Arabidopsis ; Sulfur dioxide ; Microarray analysis ; Defense-related gene ; Reactive oxygen species
• 刊名：Chemosphere
• 出版年：2012
• 期刊代码：15_00456535
• 类别：env
• 出版时间：May, 2012
• 卷：87
• 期：7
• 页码：718-724
• 文件大小：636 K

摘要

Sulfur dioxide (SO₂) is one of the most common and harmful air pollutants. To analyze cellular responses to SO₂, we investigated the transcript alterations, antioxidant enzyme activities and reactive oxygen species (ROS) levels in Arabidopsis thaliana (Col-0) exposed to SO₂. Transcriptional profiling using Affymetrix GeneChip technology identified 494 genes differentially expressed (猢?-fold change) in plants exposed to 30 mg m^鈭? SO₂ for 72 h, including up-regulation of some defense-related genes encoding antioxidant enzymes and heat shock proteins. Moreover, numerous genes encoding pathogenesis-related proteins and enzymes required for the phenylpropanoid pathway and for cell wall modification were highly activated upon SO₂ exposure. We selected eight of the significantly differentially expressed defense-related genes for analysis using semi-quantitative RT-PCR and confirmed that their expression was up-regulated under SO₂ stress. In addition, SO₂ exposure caused the enhancement of ROS production, and also increased activities of antioxidant enzymes (superoxide dismutase, peroxidase, glutathione peroxidase and glutathione S-transferase) in Arabidopsis plants. Our results indicated that increased ROS may act as a signal to induce defense responses, which provide enhanced defense capacity to protect plants against SO₂ and other environmental stress. This is the first transcriptional profiling that identifies novel genes and pathways involved in SO₂ stress responses in plant cells. These data will be helpful for better understanding the molecular basis for plant adaptation to SO₂ stress.