Echovirus 9 strain Barty non-structural protein 2C has NTPase activity

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• 作者：Klein ; Marcus ; Eggers ; Hans J. ; Nelsen-Salz ; Birgit
• 关键词：Pircornaviridae ; Enterovirus ; Echovirus 9 ; Protein 2C ; ATPase ; GTPase ; Nucleotide binding (NTB) motif
• 刊名：Virus Research
• 出版年：1999
• 期刊代码：92_01681702
• 类别：imm
• 出版时间：December 15, 1999
• 卷：65
• 期：2
• 页码：155-160
• 文件大小：201 K

摘要

Non-structural protein 2C is known to play a fundamental role in the replication of picornaviruses. Sequence analyses revealed that 2C belongs to a rapidly expanding group of proteins containing a consensus sequence for nucleotide binding (NTB). We report that echovirus 9 polypeptide 2C displays NTPase activity in vitro. In our experiments, several P2 genes were expressed in Escherichia coli as fusion proteins linked to glutathione S-transferase (GST) prior to purification close to homogeneity. In contrast to GST-2B, both GST-2C and GST-2BC showed ATPase as well as GTPase activity indicating that the site for NTB binding and splitting is located in 2C.