Quantitative analysis of perfluorinated chemicals in media for in vitro fertilization and related samples

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• 作者：Yusuke Iwasaki^a ; Emi Terayama^a ; Azusa Kato^a ; Rie Ito^a ; Koichi Saito^a ; Tsunehisa Makino^b ; ^{tmakino@k4.dion.ne.jp} ; Hiroyuki Nakazawa^a
• 关键词：Perfluorinated chemical ; In vitro fertilization ; Medium ; Human serum albumin
• 刊名：Chemosphere
• 出版年：2012
• 期刊代码：15_00456535
• 类别：env
• 出版时间：July, 2012
• 卷：88
• 期：4
• 页码：445-449
• 文件大小：380 K

摘要

The actual standard in vitro fertilization (IVF) protocol recommends an overnight gamete co-incubation. All of the culture media used for human IVF are supplemented with serum or albumin. In the present study, we determined the concentrations of perfluorinated chemicals (PFCs) in IVF media (IVFM) and related samples by liquid chromatography with tandem mass spectrometry (LC/MS/MS). The results indicated that the concentrations of PFOS and PFOA in the protein source were higher than those in the IVFM samples. Compared with human plasma concentrations of PFCs, PFCs in all of the IVFM samples, such as PFBS, PFHxS and PFOA, were either not detected or present at only trace levels, even when protein source was added. LC/MS/MS could be used to determine PFCs in IVFM samples in future studies of the effects of PFC exposure on intrauterine insemination.