Tissue expression patterns of Schistosoma mansoni Venom Allergen-Like proteins 6 and 7

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• 作者：Henrique K. Rofatto^a ; Sophia J. Parker-Manuel^b ; Tereza C. Barbosa^a ; Cibele A. Tararam^a ; R. Alan Wilson^b ; Luciana C.C. Leite^a ; Leonardo P. Farias^a ; ^{lpfarias2001@yahoo.com.br}
• 关键词：Schistosoma mansoni ; SmVAL ; Whole-mount in situ hybridisation (WISH) ; Immunocytochemistry (ICC)
• 刊名：International Journal for Parasitology
• 出版年：2012
• 期刊代码：124_00207519
• 类别：med
• 出版时间：June, 2012
• 卷：42
• 期：7
• 页码：613-620
• 文件大小：1311 K

摘要

The Schistosoma mansoni Venom Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-Coating Protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in host-pathogen interactions. Whole mount in situ hybridisation demonstrated a distinct expression pattern in oral and ventral suckers of adult worms for SmVAL6 and in the oesophageal gland for SmVAL7 transcripts, respectively. Additionally, immunocytochemistry analysis corroborated SmVAL7 expression in the oesophageal gland. Analysis of protein expression across the parasite鈥檚 life cycle revealed that the SmVAL6 protein is upregulated in cercariae and adult male worms. Furthermore, SmVAL6 protein was identified by mass spectrometry in tegument fractions of adult worms. Finally, we speculate on possible functions of these two SmVALs at the host-parasite interface.