Proliferation and differentiation of osteoblastic cells on titanium modified by ammonia plasma immersion ion implantation

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• 作者：Fei Liu^a ; Bin Li^a ; ^b ; Junying Sun^a ; ^{JYSun17@hotmail.com} ; Hongwei Li^c ; Bing Wang^a ; Shailin Zhang^a
• 关键词：Titanium ; Ammonia ; Plasma immersion ion implantation ; MC3T3-E1 cells
• 刊名：Applied Surface Science
• 出版年：2012
• 期刊代码：6_01694332
• 类别：ms
• 出版时间：1 March, 2012
• 卷：258
• 期：10
• 页码：4322-4327
• 文件大小：440 K

摘要

We report here a new method of titanium surface modification through ammonia (NH₃) plasma immersion ion implantation (PIII) technique and its effect on the cellular behaviors of MC3T3-E1 osteoblastic cells. The NH₃ PIII-treated titanium substrates (NH₃-Ti) were characterized by X-ray photoelectron (XPS), which showed that NH₃-Ti had a nitrogen-rich surface. However, there was no significant difference between the surface morphology of NH₃-Ti and unmodified Ti. When MC3T3-E1 cells were cultured on NH₃-Ti substrates, it was found that cell proliferation was accelerated at 4 and 7 days of culture. Meanwhile, cell differentiation was evaluated using type I collagen (COL I), osteocalcin (OC) and bone sialoprotein (BSP) as differentiation markers. It was found that expression of COL I and OC genes was up-regulated on NH₃-Ti substrates. However, no significant difference was found in BSP gene expression between NH₃-Ti and unmodified Ti substrates. Therefore, findings from this study indicate that surface modification of titanium through NH₃ PIII favors osteoblastic proliferation and differentiation and as a result, it may be used to improve the biocompatibility of Ti implants in vivo.