Trimethylation of histone H3K4 is associated with the induction of fructose-inducible genes in rat jejunum
- 作者：Yumiko Yoshinaga ; Kazuki Mochizuki ; Toshinao Goda ; gouda@u-shizuoka-ken.ac.jp
- 关键词：Cabp2 ; calcium binding protein 2 ; Cdkn1c ; Cyclin-dependent kinase inhibitor 1c ; ChIP ; chromatin immunoprecipitation ; CTD ; C-terminal domain ; G6pc ; glucose-6-phosphatase ; Gcgr ; glucagon receptor ; GCOS ; GeneChip operating system ; PolII ; RNA polymerase II ; R
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2012
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：23 March, 2012
- 卷：419
- 期：4
- 页码：605-611
- 文件大小：443 K
摘要
We previously reported that fructose force-feeding rapidly induces jejunal Slc2a5 gene expression in rats. In this study, we conducted microarray analyses using total RNA to identify genes upregulated in rat jejunum by fructose force-feeding. Rats were force-fed fructose, glucose or distilled water for 6 h. Genes such as Slc2a5, Cdkn1c, Cabp2, Ranbp3, Vwce and Gcgr were induced by force-feeding with fructose compared with glucose or distilled water. Chromatin immunoprecipitation assays revealed that trimethylation of histone H3K4, and acetylation of histones H3 and H4, on the transcribed region of these fructose-inducible genes were enhanced by force-feeding of fructose, but not glucose or distilled water. These results suggest that the induction of genes in the rat jejunum by fructose force-feeding is coordinately regulated by histone modifications, particularly trimethylation of histone H3K4.