Synthesis and bioactivity of fluorescence- and biotin-labeled lipid A analogues for investigation of recognition mechanism in innate immunity

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• 作者：Yukari Fujimoto ; Eiji Kimura ; Shuichi Murata ; Shoichi Kusumoto and Koichi Fukase
• 关键词：Lipid A ; Fluorescence-label ; Biotin-label ; Immunostimulation ; Innate immunity
• 刊名：Tetrahedron Letters
• 出版年：2006
• 期刊代码：109_00404039
• 类别：ch
• 出版时间：23 January 2006
• 卷：47
• 期：4
• 页码：539-543
• 文件大小：238 K

摘要

Fluorescence- and biotin-labeled lipid A analogues were synthesized for the investigation of bacterial lipopolysaccharide (LPS)/lipid A recognition in the innate immune system. For the introduction of the labeling moiety, a hydrophilic glutaryl-glucose linker was used for maintaining the bioactivity and also for preventing self-aggregation, which causes quenching of the fluorescence. We also observed the biological activity of the labeled compounds.