Studies on the UV spectrum of poly(纬-glutamic acid) based on development of a simple quantitative method

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• 作者：Wei Zeng^a ; ^b ; ¹ ; Guiguang Chen^a ; ^b ; ¹ ; Yunkai Zhang^b ; Kongyang Wu^c ; Zhiqun Liang^a ; ^b ; ^c ; ^{zqliang@gxu.edu.cn}
• 关键词：Poly(纬-glutamic acid) ; Spectrum properties ; UV spectrophotometric method ; HPLC ; High-throughput ; Biological samples
• 刊名：International Journal of Biological Macromolecules
• 出版年：2012
• 期刊代码：62_01418130
• 类别：abs
• 出版时间：July-August, 2012
• 卷：51
• 期：1-2
• 页码：83-90
• 文件大小：894 K

摘要

A simple and valid ultraviolet (UV) spectrophotometric method for the determination of poly(纬-glutamic acid) is developed. The method is based on the UV absorption spectrum of 纬-PGA in aqueous solution, which exhibits a maximum absorption wavelength at 216 nm. The results obtained were comparable to those obtained with the reported high-performance liquid chromatography (HPLC) method according to ICH guidelines. Under the proposed procedure, the calibration graph is linear over the range of 20-200 渭g/ml with regression correlation coefficient of 0.9997. Precision (%R.S.D. < 1.50) and recovery (%R. > 99.29%) are good. The limit of detection (LOD) and limit of quantitation (LOQ) are 0.39 and 1.19 渭g/ml, respectively. These results agree well with those of HPLC method. Its spectrum properties studies showed that the spectrum of 纬-PGA remarkably changed with an increase in temperature due to 纬-PGA was digested into glutamate monomer. In spite of this, the determining procedure could carried out in a wide temperature range (25-50 掳C). In addition, the method is not influenced by the molecular weight, but the measurement system need to control in pH 3.0-10.0 and ionic strength not more than 0.5 M. The proposed method is applied successfully for high-throughput quantification of poly(纬-glutamic acid) in biological samples. The advantages of the UV method are simplicity of operation, rapidity, sensitive, low-cost and high-throughput.