Protein palmitoylation inhibition by 2-bromopalmitate alters gliding, host cell invasion and parasite morphology in Toxoplasma gondii

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• 作者：A.M. Alonso ; V.M. Coceres ; M.G. De Napoli ; A.F. Nieto Guil¹ ; S.O. Angel ; M.M. Corvi ; ^{mcorvi@intech.gov.ar}
• 关键词：2-BP ; 2-bromopalmitate and 2-bromohexadecanoic acid ; AMA1 ; apical membrane antigen 1 ; RON4 ; rhoptry neck protein 4 ; ROP2 ; rhoptry protein 2 ; MIC2 ; micronemal protein 2 ; MIC3 ; micronemal protein 3 ; MIC4 ; micronemal protein 4 ; MIC7 ; micronemal protein 7 ; ML
• 刊名：Molecular and Biochemical Parasitology
• 出版年：2012
• 期刊代码：108_01666851
• 类别：bio
• 出版时间：July, 2012
• 卷：184
• 期：1
• 页码：39-43
• 文件大小：639 K

摘要

Protein palmitoylation is the reversible covalent attachment of palmitic acid onto proteins. This post-translational modification has been shown to play a part in diverse processes such as signal transduction, cellular localization and regulation of protein activity. Although many aspects of protein palmitoylation have been identified in mammalian and yeast cells, little is known of this modification in Toxoplasma gondii. In order to determine the functional role of protein palmitoylation in T. gondii, tachyzoites were treated with the palmitoylation inhibitor 2-bromopalmitate (2-BP). Parasites treated with 2-BP displayed a significant increase in non-circular trails which were longer than those trails left by non-treated parasites. Furthermore, 2-BP treatment reduced the invasion process to the host cells. Long-term treatment of intracellular tachyzoites resulted in major changes in parasite morphology and shape in a dose-dependent manner. These results suggest that palmitoylation could be modifying proteins that are key players in gliding, invasion and cytoskeletal proteins in T. gondii.