Biosynthetic study of FR-900848: unusual observation on polyketide biosynthesis that did not accept acetate as origin of acetyl-CoA

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• 作者：Hiroaki Watanabe ; Tetsuo Tokiwano and Hideaki Oikawa
• 关键词：FR-900848 ; Antifungal agent ; Biosynthesis ; Polyketide ; Polycyclopropane ; Aminonucleoside
• 刊名：Tetrahedron Letters
• 出版年：2006
• 期刊代码：109_00404039
• 类别：ch
• 出版时间：27 February 2006
• 卷：47
• 期：9
• 页码：1399-1402
• 文件大小：107 K

摘要

The biosynthetic pathway of a potent antifungal agent, FR-900848, has been examined by administration of several ¹³C-labeled precursors to Streptoverticillium fervens HP-891. Although none of the ¹³C-labeled acetate was incorporated into FR-900848, the labeling pattern of FR-900848 derived from d-[U-¹³C₆]glucose revealed that the fatty acid backbone of FR-900848 has been biosynthesized via a polyketide pathway. These unusual results strongly show that the major pathway to provide acetyl-CoA in this microorganism is glycolysis. Feeding experiments with d-[U-¹³C₆]glucose, [1,3-¹³C₂]glycerol, and l-[Me-¹³C]methionine provided information on the biosynthetic origin of structurally unusual parts (polycyclopropane and aminonucleoside) in this antibiotic.