The cave of Murci茅lagos de Zuheros (Cordoba, Spain) is one of the most important sites in Southern Iberian Peninsula. Its thick chronostratigraphic sequence includes occupations ranging from the Middle Palaeolithic to Roman times. Occupation levels corresponding to the earliest farming communities that established in modern-day Andalucia are remarkable due to its archaeological richness. This article focuses on the study of the lithic industry from these Early Neolithic levels, which is approached according to various tightly connected questions: raw material characterization, technical systems of production, and morphology and function of tools. Obtained results in their archaeological context are related to the questions on the origin of the Neolithic in Southern Iberian Peninsula.