Carbonic anhydrase activators: Activation of the human tumor-associated isozymes IX and XII with amino acids and amines
- 作者：Silvia Pastorekova ; Daniela Vullo ; Isao Nishimori ; Andrea Scozzafava ; Jaromir Pastorek ; Claudiu T. Supuran
- 关键词：Carbonic anhydrase ; Isoforms IX and XII ; Tumor ; Hypoxia ; Enzyme activator ; Amino acid ; Amine
- 刊名：Bioorganic and Medicinal Chemistry
- 出版年：2008
- 期刊代码：9_09680896
- 类别：ch
- 出版时间：1 April 2008
- 卷：16
- 期：7
- 页码：3530-3536
- 文件大小：411 K
摘要
The first activation study of the human carbonic anhydrase (hCA, EC 4.2.1.1) isoforms associated to tumors, hCA IX and XII, with a small library of natural and non-natural amino acids as well as aromatic/heterocyclic amines is reported. hCA IX was activated efficiently by dopamine, adrenaline and heterocyclic amines possessing aminoethyl-/aminomethyl-moieties (KAs of 9 nM–1.07 μM), whereas the best hCA XII activators were serotonin, l-adrenaline, 4-(2-aminoethyl)-morpholine and d-Phe (KA of 0.24–0.41 μM). Precise steric and electronic requirements are needed to be present in the molecules of effective hCA IX/hCA XII activators, in order to assure an adequate fit within the enzyme active site cavity for the formation of the enzyme–activator complex, and for an efficient proton transfer process within this complex, leading to the release of a proton and formation of the catalytically active, zinc-hydroxide species of the enzyme. Selective activation of these CA isoforms might be useful to develop pharmacologic tools or to understand whether some of these biogenic amines/amino acids may influence the progression of tumors overexpressing CA IX and/or CA XII.