CZE–ICP-MS as a tool for studying the hydrolysis of ruthenium anticancer drug candidates and their reactivity towards the DNA model compound dGMP
- 作者：Michael Groessl ; Christian G. Hartinger ; Paul J. Dyson ; Bernhard K. Keppler
- 关键词：Anticancer research ; Bioinorganic chemistry ; CZE– ; ICP-MS ; dGMP ; Hydrolysis ; Ruthenium complexes
- 刊名：Journal of Inorganic Biochemistry
- 出版年：2008
- 期刊代码：53_01620134
- 类别：ch
- 出版时间：May 2008
- 卷：102
- 期：5-6
- 页码：1060-1065
- 文件大小：254 K
摘要
Elucidating the mode of action and thereby opening the way to the design of chemotherapeutic agents is one of the major goals of metal-based anticancer research. Hydrolysis and DNA binding play an important role for pharmaceutical formulation and for exerting anticancer activity. Herein, for the first time the application of capillary zone electrophoresis–inductively-coupled plasma mass spectrometry (CZE–ICP-MS) for studying the hydrolytic stability and the binding of the ruthenium anticancer drug candidates KP418, KP1019, and RAPTA-C to dGMP is described. RAPTA-C was found to hydrolyze fastest and showed the highest reactivity toward the DNA model compound, whereas KP418 was the most stable compound in both these respects.