Preparative purification of polyethylene glycol derivatives with polystyrene-divinylbenzene beads as chromatographic packing
- 作者：Pengzhan Yu ; Xingqi Li ; Xiunan Li ; Xiuling Lu ; Guanghui Ma ; Zhiguo Su
- 关键词：Polystyrene-divinylbenzene beads ; Purification of polyethylene glycol derivatives ; Preparative reversed-phase chromatography
- 刊名：Bioorganic and Medicinal Chemistry Letters
- 出版年：2007
- 期刊代码：10_0960894x
- 类别：ch
- 出版时间：15 October 2007
- 卷：17
- 期：20
- 页码：5605-5609
- 文件大小：408 K
摘要
A clear and powerful chromatographic approach to purify polyethylene glycol derivatives at a preparative scale was reported, which was based on the polystyrene-divinylbenzene beads with ethanol/water as eluants. The validity of this method was verified with the reaction mixture of mPEG-Glu and mPEG propionaldehyde diethylacetal (ALD-PEG) as the model. The target products were one-step achieved with the purity of >99%on the polymer resins column at gram scale. The method developed was free from such disadvantages as utility of toxic solvent and narrow application scope, which was combined with conventional approaches. The method developed provided an appealing and attractive alternative methods for purification of PEG derivatives at a preparative scale.