An efficient method for extraction, separation and purification of eugenol from Eugenia caryophyllata by supercritical fluid extraction and high-speed counter-current chromatography
- 作者：Yanling Geng ; Jianhua Liu ; Ruimin Lv ; Jinpeng Yuan ; Yunliang Lin ; Xiao Wang
- 关键词：Supercritical fluid extraction (SFE) ; Counter-current chromatography ; Eugenia caryophyllata ; Eugenol ; Preparative chromatography
- 刊名：Separation and Purification Technology
- 出版年：2007
- 期刊代码：78_13835866
- 类别：ce
- 出版时间：15 October 2007
- 卷：57
- 期：2
- 页码：237-241
- 文件大小：279 K
摘要
Supercritical fluid extraction (SFE) of eugenol from Eugenia caryophyllata was performed. The optimization of parameters was carried out using an analytical-scale supercritical fluid extraction system. Then the extraction was scaled up by 60× using a preparative SFE system under the optimized conditions of 50 °C, 30 MPa and a sample particle size of 40–60 mesh. The yield of the preparative SFE was 17.1%and the yield of eugenol was 94 mg/g of dry buds. Eugenol in the extract was separated and purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (1:0.5:1:0.5, v/v). From 1.5 g of crude extract, 804 mg of eugenol was obtained at 98.5%purity as determined by HPLC.