Marker assisted pea breeding: eIF4E allele specific markers to pea seed-borne mosaic virus (PSbMV) resistance
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- 作者：Petr Sm媒kal ; Dana 艩af谩艡ov谩 ; Milan Navr谩til and Radmila Dostalov谩
- 关键词：eIF4E ; Marker assisted breeding ; Potyvirus ; Pea ; Pea seed ; borne mosaic virus ; sbm ; 1 ; Virus resistance
- 刊名：Molecular Breeding
- 出版时间：October 2010
- 出版年：2010
- 期刊代码：178_1380-3743
- 类别：bio
- 卷：26
- 期：3
- 页码：425-438
- 数据来源：sp
摘要
Traditional plant breeding relies upon crosses and subsequent selection of genotypes to meet desirable traits. The incorporation of marker-assisted selection into breeding strategies would result in a reduction in the number of offspring to be propagated, selected and tested. In the case of pea (Pisum sativum L.), the testing of resistance to viral pathogens such as pea seed-borne mosaic virus (PSbMV) is included in the breeding process. Resistance to the common strains of PSbMV is conferred by a single recessive gene (eIF4E), localized on LG VI (sbm-1 locus). We have analyzed for variation in the eIF4E genomic sequences from 43 pea varieties and breeding lines, reported as donors of resistance. This enabled a comprehensive investigation of the eIF4E gene structure and mutations responsible for PSbMV resistance were identified. Subsequently, PCR-based and gene-specific single nucleotide polymorphism and co-dominant amplicon length polymorphism markers were developed. All together 60 accessions were analyzed using sequence data and/or allele specific DNA markers. Developed allele specific markers were reproducibly amplified across a broad spectra of pea varieties and breeding lines. These were found to be 100%accurate in detecting the presence of the respective alleles when compared to symptomology and ELISA, testing (74%reliable). Hence, these molecular markers will substantially speed-up PSbMV diagnosis and resistance breeding processes in pea.