聚合酶链反应检测带状疱疹患者外周血和疱液中水痘-带状疱疹病毒
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摘要
目的:分析聚合酶链反应(PCR)检测外周血在带状疱疹诊断中的临床意义。方法:收集30例初诊的带状疱疹患者的外周血和疱液标本,通过PCR的检测外周血细胞和疱液中带状疱疹病毒,并通过免疫荧光、免疫病理进行验证。结果:PCR检测结果显示,30例患者外周血和疱液中带状疱疹病毒的核酸检测均为阳性。结论:PCR能检测外周血中的水痘-带状疱疹病毒,可用于带状疱疹的实验室诊断。
Objective: To determine the clinical significance of detecting varicella zoster in peripheral blood in the diagnosis of herpes zoster, using polymerase chain reaction(PCR). Methods: Peripheral blood and vesicular fluid were collected from 30 patients newly diagnosed with herpes zoster. Varicella zoster virus in peripheral blood cells and vesicular fluid was detected by PCR, followed by verification with immunofluorescence and immunopathology. Results: PCR showed presence of varicella zoster RNA in both peripheral blood and vesicular fluid in all 30 patients. Conclusions: PCR can detect varicella-zoster virus in peripheral blood, and can be used in the diagnosis of herpes zoster in laboratory
Objective: To determine the clinical significance of detecting varicella zoster in peripheral blood in the diagnosis of herpes zoster, using polymerase chain reaction(PCR). Methods: Peripheral blood and vesicular fluid were collected from 30 patients newly diagnosed with herpes zoster. Varicella zoster virus in peripheral blood cells and vesicular fluid was detected by PCR, followed by verification with immunofluorescence and immunopathology. Results: PCR showed presence of varicella zoster RNA in both peripheral blood and vesicular fluid in all 30 patients. Conclusions: PCR can detect varicella-zoster virus in peripheral blood, and can be used in the diagnosis of herpes zoster in laboratory
引文
[1]Ku CC,Besser J,Abendroth A,et al.Varicella-zoster virus pathogenesis and immunobiology:new concepts emerging from investigations with the SCIDhu mouse model[J].J Virol,2005,79(5):2651-2658.
[2]Chien AJ,Olerud JE.Why do so many clinicians believe that recurrent zoster is common?[J].Dermatol Online J,2007,13(2):517-524.
[3]Chaves SS,Zhang J,Civen RW,et al.Varicella disease among vaccinated persons:clinical and epidemiological characteristics,1997-2005[J].J Infect Dis,2008,197(Suppl 2):S127-S31.
[4]姜兰香,通拉嘎,庞传超,等.临床表现特殊的带状疱疹1例[J].临床皮肤科杂志,2008,37(10):645-645.
[5]陈丽娜,温海,陈江汉.216例带状疱疹临床分析[J].临床皮肤科杂志,2004,33(9):537-537.
[6]Gershon AA,Gershon MD.Pathogenesis and current approaches to control of varicella-zoster virus infections[J].Clin Microbiol Rev,2013,26(4):728-743..
[7]Asada H,Nagayama K,Okazaki A,et al.An inverse correlation of VZV skin-test reaction,but not antibody,with severity of herpes zoster skin symptoms and zoster-associated pain[J].JDermatol Sci,2013,69(3):243-249.
[8]Asano Y,Shiraki K,Takahashi M,et al.Soluble skin test antigen of varicella-zoster virus prepared from the fluid of infected cultures[J].J Infect Dis,1981,143(5):687-692.
[9]Weitzman D,Shavit O,Stein M,et al.A population based study of the epidemiology of herpes zoster and its complications[J].J Infect,2013,67(5):463-469.
[10]Arvin AM,Koropchak CM,Wittek AE.Immunologic evidence of reinfection with varicella-zoster virus[J].J Infect Dis,1983,148(2):200-205.
[11]Kronenberg A,Bossart W,Wuthrich RP,et al.Retrospective analysis of varicella zoster virus(VZV)copy DNA numbers in plasma of immunocompetent patients with herpes zoster,of immunocompromised patients with disseminated VZV disease,and of asymptomatic solid organ transplant recipients[J].Transpl Infect Dis,2005,7(3-4):116-121.
[12]Vafai A,Wellish M.Expression of varicella-zoster virus in blood mononuclear cells of patients with postherpetic neuralgia[J].Proc Natl Acad Sci USA,1988,85(8):2767-2770.
[13]Levin MJ.Varicella-zoster virus and virus DNA in the blood and oropharynx of people with latent or active varicella-zoster virus infections[J].J Clin Virol,2014,61(4):487-495.
[14]Gilden DH,Kleinschmidt-DeMasters BK,LaGuardia JJ,et al.Neurologic complications of the reactivation of varicella zoster virus[J].N Engl J Med,2000,342(9):635-645.
[15]Park KC,Yoon SS,Yoon JE,et al.A case of herpes zoster ophthalmicus with isolated trochlear nerve involvement[J].J Clin Neurol,2011,7(1):47-49.
[16]Gilden D,Cohrs RJ,Mahalingam R,et al.Varicella zoster virus vasculopathies:diverse clinical manifestations,laboratory features,pathogenesis,and treatment[J].Lancet Neurol,2009,8(8):731-740.
[17]Kronenberg A1,Bossart W,Wuthrich RP,et al.Retrospective analysis of varicella zoster virus(VZV)copy DNA numbers in plasma of immunocompetent patients with herpes zoster,of immunocompromised patients with disseminated VZV disease,and of asymptomatic solid organ transplant recipients[J].Transpl Infect Dis,2005,7(3-4):116-121.
[2]Chien AJ,Olerud JE.Why do so many clinicians believe that recurrent zoster is common?[J].Dermatol Online J,2007,13(2):517-524.
[3]Chaves SS,Zhang J,Civen RW,et al.Varicella disease among vaccinated persons:clinical and epidemiological characteristics,1997-2005[J].J Infect Dis,2008,197(Suppl 2):S127-S31.
[4]姜兰香,通拉嘎,庞传超,等.临床表现特殊的带状疱疹1例[J].临床皮肤科杂志,2008,37(10):645-645.
[5]陈丽娜,温海,陈江汉.216例带状疱疹临床分析[J].临床皮肤科杂志,2004,33(9):537-537.
[6]Gershon AA,Gershon MD.Pathogenesis and current approaches to control of varicella-zoster virus infections[J].Clin Microbiol Rev,2013,26(4):728-743..
[7]Asada H,Nagayama K,Okazaki A,et al.An inverse correlation of VZV skin-test reaction,but not antibody,with severity of herpes zoster skin symptoms and zoster-associated pain[J].JDermatol Sci,2013,69(3):243-249.
[8]Asano Y,Shiraki K,Takahashi M,et al.Soluble skin test antigen of varicella-zoster virus prepared from the fluid of infected cultures[J].J Infect Dis,1981,143(5):687-692.
[9]Weitzman D,Shavit O,Stein M,et al.A population based study of the epidemiology of herpes zoster and its complications[J].J Infect,2013,67(5):463-469.
[10]Arvin AM,Koropchak CM,Wittek AE.Immunologic evidence of reinfection with varicella-zoster virus[J].J Infect Dis,1983,148(2):200-205.
[11]Kronenberg A,Bossart W,Wuthrich RP,et al.Retrospective analysis of varicella zoster virus(VZV)copy DNA numbers in plasma of immunocompetent patients with herpes zoster,of immunocompromised patients with disseminated VZV disease,and of asymptomatic solid organ transplant recipients[J].Transpl Infect Dis,2005,7(3-4):116-121.
[12]Vafai A,Wellish M.Expression of varicella-zoster virus in blood mononuclear cells of patients with postherpetic neuralgia[J].Proc Natl Acad Sci USA,1988,85(8):2767-2770.
[13]Levin MJ.Varicella-zoster virus and virus DNA in the blood and oropharynx of people with latent or active varicella-zoster virus infections[J].J Clin Virol,2014,61(4):487-495.
[14]Gilden DH,Kleinschmidt-DeMasters BK,LaGuardia JJ,et al.Neurologic complications of the reactivation of varicella zoster virus[J].N Engl J Med,2000,342(9):635-645.
[15]Park KC,Yoon SS,Yoon JE,et al.A case of herpes zoster ophthalmicus with isolated trochlear nerve involvement[J].J Clin Neurol,2011,7(1):47-49.
[16]Gilden D,Cohrs RJ,Mahalingam R,et al.Varicella zoster virus vasculopathies:diverse clinical manifestations,laboratory features,pathogenesis,and treatment[J].Lancet Neurol,2009,8(8):731-740.
[17]Kronenberg A1,Bossart W,Wuthrich RP,et al.Retrospective analysis of varicella zoster virus(VZV)copy DNA numbers in plasma of immunocompetent patients with herpes zoster,of immunocompromised patients with disseminated VZV disease,and of asymptomatic solid organ transplant recipients[J].Transpl Infect Dis,2005,7(3-4):116-121.