基于多指标评价技术研究云南不同产地玛咖的质量
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摘要
【目的】以玛咖功效物质(玛咖酰胺、总生物碱、玛咖多糖)为主要指标,建立多指标评价体系对云南不同产地玛咖质量进行综合性评价。【方法】采用蒽酮-硫酸比色法检测玛咖多糖含量,酸性染料比色法检测玛咖总生物碱的含量,高效液相色谱法检测玛咖酰胺的含量。【结果】云南不同产地的玛咖主要化学物质有显著差异,德钦产区的玛咖多糖(8.6%)、玛咖总生物碱(3.18 mg/g)及玛咖酰胺总量(2.46 mg/g)含量均为最高,且德钦、维西地区的玛咖质量显著高于其他地区。【结论】云南不同产地玛咖在主要化学物质(玛咖酰胺、总生物碱、玛咖多糖)含量上体现出的这种显著性差异,究其原因与其产地的海拔和温度密切相关。
【Objective】The quality of Lepidium meyenii Walp. in different areas of Yunnan province was evaluated according to the contents of the effective substances(polysaccharide, total alkaloid and macamides) of L. Meyenii. A multi-index evaluation system was established.【Method】The contents of polysaccharide, total alkaloid and macamides were determined by anthrone-sulfuric acid colorimetry, acidic dye colorimetry and high performance liquid chromatography, respectively.【Result】The results showed that the effective substances contents of L. Meyenii from different areas in Yunnan province were significantly different. The contents of polysaccharide, total alkaloids and total macamides were the highest in the Deqin area, which were 8.6 %, 3.18 mg/g and 2.46 mg/g, respectively, and the quality of L. meyenii in Deqin and Weixi areas was significantly better than that in other areas.【Conclusion】The significant difference existing in the contents of the effective substances(polysaccharide, total alkaloid and macamides) of L. Meyenii. was related to the altitude and temperature of producing areas.
【Objective】The quality of Lepidium meyenii Walp. in different areas of Yunnan province was evaluated according to the contents of the effective substances(polysaccharide, total alkaloid and macamides) of L. Meyenii. A multi-index evaluation system was established.【Method】The contents of polysaccharide, total alkaloid and macamides were determined by anthrone-sulfuric acid colorimetry, acidic dye colorimetry and high performance liquid chromatography, respectively.【Result】The results showed that the effective substances contents of L. Meyenii from different areas in Yunnan province were significantly different. The contents of polysaccharide, total alkaloids and total macamides were the highest in the Deqin area, which were 8.6 %, 3.18 mg/g and 2.46 mg/g, respectively, and the quality of L. meyenii in Deqin and Weixi areas was significantly better than that in other areas.【Conclusion】The significant difference existing in the contents of the effective substances(polysaccharide, total alkaloid and macamides) of L. Meyenii. was related to the altitude and temperature of producing areas.
引文
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[2]Li L,Zhou S S.Nutrition,food security assessment and development prospects of Maca[J].Sci Tec Food Industry,2012(5):376-379.
[3]Li J,Sun Q,Meng Q,et al.Anti-fatigue activity of polysaccharide fractions from Lepidium meyenii Walp.(maca) [J].Int J Biol Macromol,2017,95(2):1305-1311.
[4]Gan J.Study on antioxidant activities and antioxidative substance basis of maca [D].Beijing:Chinese Academy of Forestry,2013.
[5]Renata M C,Monica L,Helena H,et al.Medicinal plants as alternative treatments for female sexual dysfunction:utopian vision or possible treatment in climactrric women[J].J Sex Med,2010,7(11):3695.
[6]Du G X.Study on the isolation,purification and antioxidate activities of alkaloids in maca[D].Guangzhou:South China University of Technology,2011.
[7]Zheng B L,He K,Kim C H,et al.Effect of a lipidie extract from Lepidium meyenii on sexual behavior in mice and rats[J].Urology,2000,55(4):598-602.
[8]Rubio J,Yucra S,Gasco M,et al.Dose-response effect of blackmaca (Lepidium meyenii) in mice with memory impairment induced by ethanol [J].Toxicol Mech Methods,2011,21(8):628-634.
[9]Alquraini A,Waggas D,B?hlke M,et al.Neuroprotective effects of Lepidium meyenii (Maca) and macamides against amyloid-beta (25-35) induced toxicity in B-35 neuroblastoma cells [J].FASEB J,2014,28(1):657.13.
[10]Hajdu Z,Nicolussi S,Rau M,et al.Identification ofendocannabinoid system-modulating N-alkylamides from Heliopsis helianthoides var.scabra and Lepidium meyenii[J].J Nat Prod,2014,77(7):1663-1669.
[11]Almukadi H,Wu H,B?hlke M,et al.The macamide N-3-methoxybenzyl-linoleamide is a time-dependent fattyacid amide hydrolase (FAAH) inhibitor [J].Mol Neurobiol,2013,48(2):333-339.
[12]Liu Y J.Anti-fatigue effects of N-benzyl-hexadecamide and extract in mice[J].Chin J Pulic Health,2015,34:92-93.
[13]陈金金,赵兵.玛咖酰胺研究进展[J].中草药,2015,46(21):3284-3288.
[14]曾学健,沈涛,胡继学,等.不同品系铁皮石斛茎叶生物量分配特征及多糖含量比较[J].西南农业学报,2018,31(1):57-61.
[15]王婧,邓辰辰,徐振秋,等.玛咖总生物碱检测方法的建立及提取优化[J].食品工业科技,2016,37(2):73-81.
[16]朱颖秋,邓小宽,沈洋,等.同产地玛咖中酰胺含量分析[J].天然产物研究与开发,2014,26:1982-1985.
[17]夏陈,邓俊琳,张盈娇,等.高效液相法测定川产中玛咖N-苄基-十六烷酰胺的含量[J].化学与生物工程,2017,34(2):68-70.