阿特拉津高效降解菌株DnL1-1发酵培养基的优化
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摘要
为提高产脲节杆菌DnL1-1的发酵水平,通过单因子筛选及正交试验,优化了DnL1-1的发酵培养基。优化得到的培养基配方为:葡萄糖15. 0 g/L,蛋白胨7. 5 g/L,酵母提取物0. 50 g/L,磷酸氢二钾2. 0 g/L,磷酸二氢钾1. 0 g/L,氯化钙0. 15 g/L,硫酸镁0. 15 g/L。配方在接种量为1%,180 r/min,28℃摇瓶振荡培养至36 h时,菌体数量达2. 89×1010cfu/m L以上。
In order to improve the biomass of Arthrobacter ureafaciens DnL1-1,the fermentation medium of the strain DnL1-1 was optimized by single factor screening and orthogonal experiment. The results showed that the best formula should be: glucose 15. 0 g/L,peptone 7. 5 g/L,yeast extract 0. 50 g/L,K2 HPO42. 0 g/L,KH2 PO41. 0 g/L,Ca Cl20. 15 g/L,MgSO40. 15 g/L. Thus the strain can produce about 2. 89 × 1010 cells in 1 mL of the culture medium under the condition of1% inoculum,with shaking of 180 r/min at 28 ℃ for 36 hours.
In order to improve the biomass of Arthrobacter ureafaciens DnL1-1,the fermentation medium of the strain DnL1-1 was optimized by single factor screening and orthogonal experiment. The results showed that the best formula should be: glucose 15. 0 g/L,peptone 7. 5 g/L,yeast extract 0. 50 g/L,K2 HPO42. 0 g/L,KH2 PO41. 0 g/L,Ca Cl20. 15 g/L,MgSO40. 15 g/L. Thus the strain can produce about 2. 89 × 1010 cells in 1 mL of the culture medium under the condition of1% inoculum,with shaking of 180 r/min at 28 ℃ for 36 hours.
引文
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[15]李红梅,李成云,李纪顺,等.产脲节杆菌Dn L1-1与植物联合对阿特拉津的降解[J].科学技术与工程,2017,17(17):357-360.
[16]雷丽萍,夏振远,郭荣君,等.降烟碱节杆菌发酵条件研究[J].中国烟草科学,2006,27(3):45-47.
[17]李娜,雷丽萍,夏振远,等.节杆菌K15发酵条件的研究[J].云南农业大学学报,2010,25(6):817-826.
[2]王英姿,纪明山,黄国宏,等.土壤中莠去津对几种农作物安全临界浓度的确定[J].沈阳农业大学学报,2002,33(1):33-34.
[3]DALTON R. Frogs put in the gender blender by America’s favourite herbicides[J]. Nature,2002,416:665-666.
[4]MURPHYA M B,HECKE M,COADY K K,et al. Atrazine concentrations,gonadal gross morphology and histology in ranid frogs collected in Michigan agricultural areas[J]. Aquatic Toxicology,2006,76:230-245.
[5]PROSEN H,ZUPANCIC-KRALJ L. Evaluation of photolysis and hydrolysis of atrazine and itsfirst degradation products in the presence of humic acids[J]. Environmental Pollution,2005,133:517-529.
[6]GETENGA Z,DRFLER U,LWOBI A,et al. Atrazine and terbuthylazine mineralization by an Arthrobacter sp. Isolated from a sugarcane-cultivated soil in Kenya[J]. Chemosphere,2009,77:534-539.
[7]de SOUZA M L,WACKETT L P,SADOWSKY M J. The atz ABC genes encoding atrazine catabolism are located on a selftransmissible plasmid in Pseudomonas sp. strain ADP[J]. Appl Environ Microbiol,1998,64:2323-2326.
[8]ZHANG Y,GE S J,JIANG M Y,et al. Combined bioremediation of atrazine-contaminated soil by Pennisetum and Arthrobacter sp. strain DNS10[J]. Environ Sci Pollut Res,2014,21(9):6234-6238.
[9]BAZHANOV D P,LI C Y,LI H M,et al. Occurrence,diversity and community structure of culturable atrazine degraders in industrial and agricultural soils exposed to the herbicide in Shandong Province,P. R. China[J]. BMC Microbiol. 2016,16:265.
[10]李红梅,李成云,李纪顺,等.阿特拉津降解细菌的直接检测及分离[J].山东科学,2015,28(6):17-22.
[11]韩鹏,洪青,何丽娟,等.阿特拉津降解菌ADH-2的分离、鉴定及其特性研究[J].农业环境科学学报,2009,28(2):406-410.
[12]SAJJAPHAN K,HEEPNGOEN P,SADOWSKY M J,et al. Arthrobacter sp. strain KU001 isolated from a Thai soil degrades atrazine in the presence of inorganic nitrogen sources[J]. J Microbiol Biotechnol,2010,20(3):602-608.
[13]WANG J H,ZHU L S,LIU A J,et al. Isolation and characterization of an Arthrobacter sp. Strain HB-5 that transforms atrazine[J]. Environ Geochem Health,2011,33:259-266.
[14]刘春光,杨峰山,卢星忠,等.阿特拉津降解菌T3AB1的分离鉴定及土壤修复[J].微生物学报,2010,50(12):1642-1650.
[15]李红梅,李成云,李纪顺,等.产脲节杆菌Dn L1-1与植物联合对阿特拉津的降解[J].科学技术与工程,2017,17(17):357-360.
[16]雷丽萍,夏振远,郭荣君,等.降烟碱节杆菌发酵条件研究[J].中国烟草科学,2006,27(3):45-47.
[17]李娜,雷丽萍,夏振远,等.节杆菌K15发酵条件的研究[J].云南农业大学学报,2010,25(6):817-826.