HPLC法测定芩杏清热止咳颗粒中8种成分
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摘要
目的建立HPLC法测定芩杏清热止咳颗粒(黄芩、栀子、桑叶等)中8种成分的含有量。方法黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的分析采用Diamonsil Plus C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相0.2%磷酸-甲醇,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长274 nm。栀子苷、甘草苷、异甘草苷、甘草酸的分析采用Diamonsil Plus C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相0.05%磷酸-乙腈,梯度洗脱;体积流量1.0 mL/min;柱温25℃;检测波长237、360 nm。结果 8种成分在各自范围内线性关系良好(r>0.999 5),平均加样回收率97.35%～98.73%,RSD 0.61%～1.9%。结论该方法准确可靠,重复性好,可用于芩杏清热止咳颗粒的质量控制。
AIM To establish an HPLC method for the content determination of eight constituents in Qinxing Qingre Zhike Granules(Scutellariae Radix, Gardeniae Fructus, Mori Folium, etc.).METHODS The analyses of baicalin, wogonoside, baicalein and wogonin were performed on a 25 ℃ thermostatic Diamonsil Plus C_(18)column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of 0.2% phosphoric acid-methanol flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 274 nm. The analyses of geniposide, liquritin, isoliquiritin and glycyrrhizic acid were performed on a 25 ℃ thermostatic Diamonsil Plus C_(18)column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of 0.05% phosphoric acid-acetonitrile flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelengths were set at 237, 360 nm.RESULTS Eight constituents showed good linear relationships within their own ranges(r>0.999 5), whose average recoveries were 97.35%-98.73% with the RSDs of 0.61%-1.9%.CONCLUSION This accurate, reliable and reproducible method can be used for the quality control of Qinxing Qingre Zhike Granules.
AIM To establish an HPLC method for the content determination of eight constituents in Qinxing Qingre Zhike Granules(Scutellariae Radix, Gardeniae Fructus, Mori Folium, etc.).METHODS The analyses of baicalin, wogonoside, baicalein and wogonin were performed on a 25 ℃ thermostatic Diamonsil Plus C_(18)column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of 0.2% phosphoric acid-methanol flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 274 nm. The analyses of geniposide, liquritin, isoliquiritin and glycyrrhizic acid were performed on a 25 ℃ thermostatic Diamonsil Plus C_(18)column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of 0.05% phosphoric acid-acetonitrile flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelengths were set at 237, 360 nm.RESULTS Eight constituents showed good linear relationships within their own ranges(r>0.999 5), whose average recoveries were 97.35%-98.73% with the RSDs of 0.61%-1.9%.CONCLUSION This accurate, reliable and reproducible method can be used for the quality control of Qinxing Qingre Zhike Granules.
引文
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[2] Chinnam N,Dadi P K,Sabri S A,et al.Dietary bioflavonoids inhibit Escherichia coli ATP synthase in a differential manner[J].Int J Biol Macromol,2010,46(5):478-486.
[3] 宋成岩,刘宇,朴锦花,等.黄芩抗氧化化学成分的研究[J].时珍国医国药,2007,18(4):856-857.
[4] Hussain I,Waheed S,Ahmad K A,et al.Scutellaria baicalensis targets the hypoxia-inducible factor-1alpha and enhances cisplatin efficacy in ovarian cancer[J].J Cell Biochem,2018,119(9):7515-7524.
[5] 杨晓露,刘朵,卞卡,等.甘草总黄酮及其成分体外抗炎活性及机制研究[J].中国中药杂志,2013,38(1):99-104.
[6] 李阳,高欢,朱庆均,等.甘草化学成分抗病毒活性研究进展[J].山东中医杂志,2017,36(2):167-171.
[7] 万嘉洋,杨洁红,虞立,等.甘草次酸、甘草苷配伍次乌头碱对慢性心衰大鼠细胞凋亡通路的影响[J].中成药,2017,39(10):2018-2023.
[8] 李敏,王斌,唐志书,等.黄芩苷、栀子苷对大鼠脑缺血保护作用的机制[J].中药药理与临床,2012,28(3):34-36.
[9] 彭婕,钱之玉,刘同征,等.京尼平苷和西红花酸保肝利胆作用的比较[J].中国新药杂志,2003,12(2):105-108.
[10] 武海霞.栀子苷体外抗氧化作用[J].中国药理学与毒理学杂志,2012,26(3):456.
[11] 陆丽娜,董加花,左艳华,等.RP-HPLC法同时测定清肺化痰丸中4种成分[J].中成药,2016,38(9):1956-1959.
[12] 朱友平,苏中武,李承祜.苦杏仁甙的镇痛作用和无身体依赖性[J].中国中药杂志,1994,19(2):105-107.
[13] 俞灵莺,李向荣,方晓.桑叶总黄酮对糖尿病大鼠小肠双糖酶的抑制作用[J].中华内分泌代谢杂志,2002,18(4):313-315.
[14] Oku T,Yamada M,Nakamura M,et al.Inhibitory effects of extractives from leaves of Morus alba on human and rat small intestinal disaccharidase activity[J].Br J Nutr,2006,95(5):933-938.
[15] 郑勇凤,王佳婧,傅超美,等.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.
[16] 刘清华.甘草的化学成分和药理作用的概述[J].中国中医药现代远程教育,2011,9(13):84.