可切换亲水溶剂高效提取藻油及岩藻黄素含量分析
|
摘要
采用可切换亲水溶剂N,N-二甲基环己胺(DMCHA)从湿三角褐指藻藻泥以及干粉中通过非蒸馏的节能方式提取藻油,并比较DMCHA法与氯仿甲醇法及乙醇法提取的藻油岩藻黄素总含量。结果表明:在料液比1∶25、提取时间24 h条件下,三角褐指藻干粉藻油得率达到21. 29%,三角褐指藻藻泥的藻油得率达到13. 29%; SEM观察发现提油后三角褐指藻表面结构有明显的破坏;DMCHA法提取的藻油中岩藻黄素总含量为9. 17 mg/g,与氯仿甲醇法相差不大,低于乙醇法提取的藻油中岩藻黄素总含量;核磁分析发现,藻油中的溶剂残留仅为提取溶剂添加量的0. 231%,测定溶剂回收率为93. 2%。
The switchable hydrophilic solvent DMCHA was used to extract algae oil from both algae mud and dry powder of Phaeodactylum tricornutum without distillation process,and the fucoxanthin contents in algae oils extracted by DMCHA method,Folch method and ethanol method were compared. The results showed that under the conditions of ratio of solid to liquid 1∶ 25 and extraction time 24 h,the extraction rates of algae oil from dry powder and algae mud were 21. 29% and 13. 29% respectively. After the oil extraction,the surface structure of Phaeodactylum tricornutum was obviously damaged. The fucoxanthin content in algae oil extracted by DMCHA method was 9. 17 mg/g,which was close to Folch method and lower than ethanol method. It was found by nuclear magnetic analysis that the solvent residue in the algae oil was only 0. 231% of the addition amount of the extraction solvent,and the recovery rate of solvent was 93. 2%.
The switchable hydrophilic solvent DMCHA was used to extract algae oil from both algae mud and dry powder of Phaeodactylum tricornutum without distillation process,and the fucoxanthin contents in algae oils extracted by DMCHA method,Folch method and ethanol method were compared. The results showed that under the conditions of ratio of solid to liquid 1∶ 25 and extraction time 24 h,the extraction rates of algae oil from dry powder and algae mud were 21. 29% and 13. 29% respectively. After the oil extraction,the surface structure of Phaeodactylum tricornutum was obviously damaged. The fucoxanthin content in algae oil extracted by DMCHA method was 9. 17 mg/g,which was close to Folch method and lower than ethanol method. It was found by nuclear magnetic analysis that the solvent residue in the algae oil was only 0. 231% of the addition amount of the extraction solvent,and the recovery rate of solvent was 93. 2%.
引文
[1]SANG M K,JUNG Y J,KWON O N,et al.A potential commercial source of fucoxanthin extracted from the microalga Phaeodactylum tricornutum[J].Appl Biochem Biotechnol,2012,166(7):1843-1855.
[2]MCCLURE D D,LUIZ A,GERBER B,et al.An investigation into the effect of culture conditions on fucoxanthin production using the marine microalgae Phaeodactylum tricornutum[J].Algal Res,2018,29:41-48.
[3]PENG J,YUAN J P,WU C F,et al.Fucoxanthin,a marine carotenoid present in brown seaweeds and diatoms:metabolism and bioactivities relevant to human health[J].Mar Drugs,2011,9(10):1806-1828.
[4]PARISA H,ISABELLE P,DAMIEN L,et al.Plastids of marine phytoplankton produce bioactive pigments and lipids[J].Mar Drugs,2013,11(9):3425-3471.
[5]MAEDA H,HOSOKAWA M,SASHIMA T,et al.Fucoxanthin from edible seaweed,Undaria pinnatifida,shows antiobesity effect through UCP1 expression in white adipose tissues[J].Biochem Biophys Res Commun,2005,332(2):392-397.
[6]翟量,沈立荣.富含EPA微藻油超声波辅助溶剂法提取工艺优化及脂肪酸成分分析[J].食品安全质量检测学报,2016,7(2):809-815.
[7]曲孟.海水小球藻絮凝采收与藻油提取工艺的初步试验研究[D].辽宁大连:大连海洋大学,2014.
[8]JESSOP P G,HELDEBRANT D J,LI X,et al.Green chemistry:reversible nonpolar-to-polar solvent[J].Nature,2005,436:1102.
[9]JESSOP P G,KOZYCZ L,RAHAMI Z G,et al.Tertiary amine solvents having switchable hydrophilicity[J].Green Chem,2011,13(3):619-623.
[10]BOYD A R,CHAMPAGNE P,MCGINN P J,et al.Switchable hydrophilicity solvents for lipid extraction from microalgae for biofuel production[J].Bioresour Technol,2012,118:628-632.
[11]HUANG W C,LIU H,SUN W,et al.Effective astaxanthin extraction from wet Haematococcus pluvialis using switchable hydrophilicity solvents[J].ACS Sust Chem Eng,2018,6(2):1560-1563.
[12]HONDOH H,YAMASAKI K,IKUTAKE M,et al.Visualization of oil migration in chocolate using scanning electron microscopy-energy dispersive X-ray spectroscopy[J].Food Struct,2016,8:8-15.
[13]SAMORC,BARREIRO D L,VET R,et al.Effective lipid extraction from algae cultures using switchable solvents[J].Green Chem,2013,15(2):353-356.
[14]孙维维,姜晓明,徐杰,等.太平洋磷虾油提取工艺优化及与南极磷虾油品质的比较[J].中国油脂,2018,43(1):8-12.
[15]曹文静,惠欢庆,沈俊涛,等.混合溶剂提取南极磷虾油的工艺研究[J].中国油脂,2013,38(12):6-9.
[16]WANG W,WANG G,MING Z,et al.Isolation of fucoxanthin from the rhizoid of Laminaria japonica Aresch[J].J Integrative Plant Biol,2005,47(8):1009-1015.
[17]秦云,孟丽媛,王凤舞.复合酶法提取海带岩藻黄素及其抗氧化活性分析[J].食品科学,2013,34(16):279-283.
[2]MCCLURE D D,LUIZ A,GERBER B,et al.An investigation into the effect of culture conditions on fucoxanthin production using the marine microalgae Phaeodactylum tricornutum[J].Algal Res,2018,29:41-48.
[3]PENG J,YUAN J P,WU C F,et al.Fucoxanthin,a marine carotenoid present in brown seaweeds and diatoms:metabolism and bioactivities relevant to human health[J].Mar Drugs,2011,9(10):1806-1828.
[4]PARISA H,ISABELLE P,DAMIEN L,et al.Plastids of marine phytoplankton produce bioactive pigments and lipids[J].Mar Drugs,2013,11(9):3425-3471.
[5]MAEDA H,HOSOKAWA M,SASHIMA T,et al.Fucoxanthin from edible seaweed,Undaria pinnatifida,shows antiobesity effect through UCP1 expression in white adipose tissues[J].Biochem Biophys Res Commun,2005,332(2):392-397.
[6]翟量,沈立荣.富含EPA微藻油超声波辅助溶剂法提取工艺优化及脂肪酸成分分析[J].食品安全质量检测学报,2016,7(2):809-815.
[7]曲孟.海水小球藻絮凝采收与藻油提取工艺的初步试验研究[D].辽宁大连:大连海洋大学,2014.
[8]JESSOP P G,HELDEBRANT D J,LI X,et al.Green chemistry:reversible nonpolar-to-polar solvent[J].Nature,2005,436:1102.
[9]JESSOP P G,KOZYCZ L,RAHAMI Z G,et al.Tertiary amine solvents having switchable hydrophilicity[J].Green Chem,2011,13(3):619-623.
[10]BOYD A R,CHAMPAGNE P,MCGINN P J,et al.Switchable hydrophilicity solvents for lipid extraction from microalgae for biofuel production[J].Bioresour Technol,2012,118:628-632.
[11]HUANG W C,LIU H,SUN W,et al.Effective astaxanthin extraction from wet Haematococcus pluvialis using switchable hydrophilicity solvents[J].ACS Sust Chem Eng,2018,6(2):1560-1563.
[12]HONDOH H,YAMASAKI K,IKUTAKE M,et al.Visualization of oil migration in chocolate using scanning electron microscopy-energy dispersive X-ray spectroscopy[J].Food Struct,2016,8:8-15.
[13]SAMORC,BARREIRO D L,VET R,et al.Effective lipid extraction from algae cultures using switchable solvents[J].Green Chem,2013,15(2):353-356.
[14]孙维维,姜晓明,徐杰,等.太平洋磷虾油提取工艺优化及与南极磷虾油品质的比较[J].中国油脂,2018,43(1):8-12.
[15]曹文静,惠欢庆,沈俊涛,等.混合溶剂提取南极磷虾油的工艺研究[J].中国油脂,2013,38(12):6-9.
[16]WANG W,WANG G,MING Z,et al.Isolation of fucoxanthin from the rhizoid of Laminaria japonica Aresch[J].J Integrative Plant Biol,2005,47(8):1009-1015.
[17]秦云,孟丽媛,王凤舞.复合酶法提取海带岩藻黄素及其抗氧化活性分析[J].食品科学,2013,34(16):279-283.