反硝化聚磷菌的培养及其脱氮除磷特性
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摘要
采用SBR反应器驯化培养反硝化聚磷菌,考察了厌氧-缺氧-好氧和厌氧-缺氧运行模式下反硝化聚磷菌的增殖情况、反应器的脱氮除磷特性及胞内聚合物聚β-羟基丁酸(PHB)和糖原的合成消耗情况。实验结果表明:经过72 d(288个周期)的驯化培养,SBR反应器内反硝化聚磷菌的数量约占全部聚磷菌的84.5%;厌氧-缺氧培养方式下,反硝化聚磷菌的释磷速率为34.5 mg/(L·h),缺氧吸磷速率为23.0 mg/(L·h),缺氧阶段每降解1.0 mg/L的PO_4~(3-)-P需要消耗1.0 mg/L的NO_3~--N;每消耗6.3 mg/L的COD生成1 mg/g的PHB,每降解1 mol的PHB约生成0.73 mol的糖原。
Denitrifying phosphorus accumulating organisms(DPAOs)were cultured in a sequencing batch reactor(SBR). Under the anaerobic-anoxic-aerobic and anaerobic-anoxic mode,the proliferation of DPAOs,the nitrogen and phosphorus removal characteristics of the reactor and the synthesis and consumption of intracellular polymers poly-β-hydroxybutyrate(PHB)and glycogen in the microorganisms were investigated. The experimental results showed that after 72 d(288 cycles)of acclimation,DPAOs in the SBR reached 84.5% of the total phosphorus accumulating organisms(PAOs);Under the anaerobic-anoxic mode,the phosphorus release rate of DPAOs was 34.5 mg/(L·h),the phosphorus removal rate at the anoxic stage reached 23.0 mg/(L·h),and 1 mg/L NO_3~--N was consumed for degradation of 1 mg/L PO_4~(3-)P;1 mg/g PHB was synthesized when 6.3 mg/L COD was consumed,and about 0.73 mol glycogen was synthesized when 1 mol PHB was degraded.
Denitrifying phosphorus accumulating organisms(DPAOs)were cultured in a sequencing batch reactor(SBR). Under the anaerobic-anoxic-aerobic and anaerobic-anoxic mode,the proliferation of DPAOs,the nitrogen and phosphorus removal characteristics of the reactor and the synthesis and consumption of intracellular polymers poly-β-hydroxybutyrate(PHB)and glycogen in the microorganisms were investigated. The experimental results showed that after 72 d(288 cycles)of acclimation,DPAOs in the SBR reached 84.5% of the total phosphorus accumulating organisms(PAOs);Under the anaerobic-anoxic mode,the phosphorus release rate of DPAOs was 34.5 mg/(L·h),the phosphorus removal rate at the anoxic stage reached 23.0 mg/(L·h),and 1 mg/L NO_3~--N was consumed for degradation of 1 mg/L PO_4~(3-)P;1 mg/g PHB was synthesized when 6.3 mg/L COD was consumed,and about 0.73 mol glycogen was synthesized when 1 mol PHB was degraded.
引文
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[3]吕永涛,张瑶,闫建平,等.电子受体及投加方式对反硝化除磷及N2O释放影响[J].水处理技术,2017(12):38-42.
[4]宋成康,秦健,王亚宜,等.碳源对反硝化除磷效能及N2O产生的长期影响[J].环境污染与防治,2014(12):11-16.
[5]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社,2002.
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[8]WACHTMEISTER A,KUBA T,VAN LOOSDRECH M C M,et a1. A sludge characterization assay for aerobic and denitrifying phosphorus removing sludge[J].Water Res,1997,31(3):471-478.
[9]方茜,张朝升,张立秋,等.基于PHB碳源驱动的同时硝化/反硝化除磷过程[J].环境科学学报,2014,34(8):1968-1977.
[10]王景峰,王暄,季民,等.聚糖菌颗粒污泥基于胞内储存物质的同步硝化反硝化[J].环境科学,2006,27(3):3473-3477.
[11]戴娴,彭永臻,王晓霞,等.不同厌氧时间对富集聚磷菌的SNDPR系统处理性能的影响[J].中国环境科学,2016,36(1):92-99.