TGF-β1和Smad7在口腔鳞状细胞癌中的表达及意义
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摘要
目的探讨转化生长因子β1(TGF-β1)、Smad7在口腔鳞状细胞癌(OSCC)、正常口腔黏膜组织中的表达和意义。方法通过免疫组织化学SP法检测TGF-β1、Smad7在31例口腔鳞状细胞癌组织和10例正常口腔黏膜组织中的表达,探讨TGF-β1、Smad7在口腔鳞状细胞癌中的表达及相关性因素。结果 TGF-β1、Smad7在口腔鳞状细胞癌中的阳性率高于正常口腔黏膜组织(P<0.05),二者在口腔鳞癌有淋巴结转移组的表达率明显高于无淋巴结转移组的表达率(P<0.05),二者在口腔鳞癌TNM分期为Ⅰ+Ⅱ期中的阳性表达率低于Ⅲ+Ⅳ期的阳性表达率(P<0.05),与患者年龄和性别无关。TGF-β1和Smad7在口腔鳞癌中的表达呈显著正相关(r=0.379,P<0.05)。结论 TGF-β1、Smad7在口腔鳞状细胞癌组织中呈过表达,可能作为判断口腔鳞状细胞癌预后的指标。
Objective To investigate the expression and significance of transforming growth factor beta 1 and Smad7 in oral squamous cell carcinoma. Methods A total of 31 cases of oral squamous cell carcinoma and 10 cases of normal oral mucosa were included. The expression of TGF-β1 and Smad7 was detected by immunohistochemical staining. Results The positive rate of TGF-β1 and Smad7 in oral squamous cell carcinoma was higher than that of normal oral mucosa(P<0.05), the expression in cases with lymph node metastasis was significantly higher than those without lymph node metastasis(P<0.05) and lower in stage stage I+Ⅱ than in stage Ⅲ + Ⅳ(P<0.05). The expression was not related to age and gendere. The expression of TGF-β1 and Smad7 in oral squamous cell carcinoma showed significant positive correlation(r=0.379, P<0.05). Conclusion The expression of TGF-β1 and Smad7 in oral squamous cell carcinoma was up regulated, which may serve as an indicator of prognosis of oral squamous cell carcinoma.
Objective To investigate the expression and significance of transforming growth factor beta 1 and Smad7 in oral squamous cell carcinoma. Methods A total of 31 cases of oral squamous cell carcinoma and 10 cases of normal oral mucosa were included. The expression of TGF-β1 and Smad7 was detected by immunohistochemical staining. Results The positive rate of TGF-β1 and Smad7 in oral squamous cell carcinoma was higher than that of normal oral mucosa(P<0.05), the expression in cases with lymph node metastasis was significantly higher than those without lymph node metastasis(P<0.05) and lower in stage stage I+Ⅱ than in stage Ⅲ + Ⅳ(P<0.05). The expression was not related to age and gendere. The expression of TGF-β1 and Smad7 in oral squamous cell carcinoma showed significant positive correlation(r=0.379, P<0.05). Conclusion The expression of TGF-β1 and Smad7 in oral squamous cell carcinoma was up regulated, which may serve as an indicator of prognosis of oral squamous cell carcinoma.
引文
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3 Zhao B,Chen YG.Regulation of TGF-βsignal transduction.Scientifica(Cairo),2014,2014:874065.
4蔡传宝,陶学金,朱声荣,等.TGF-β1、Smad4和Cyclin D1在口腔鳞癌中的表达和意义.口腔医学研究,2007,23(1):14-16.
5 Cheung DY,Kim JK.Perspectives of the stomach cancer treat-ment:the introduction of molecular targeted therapy and the hope for cure.Korean J Gastroenterol,2013,61(3):117-127.
6张晓金,归绥琪.宫颈癌发病机制的研究进展.中国妇幼健康研究,2008,19(1):56-59.
7 Katz LH,Likhter M,Jogunoori W,et al.TGF-βsignaling in liver and gastrointestinal cancers.Cancer Lett.2016,379(2):166-172.
8 Galie PA,Russell MW,Westfall MV,et al.Interstitial fluid flow and cyclic strain differentially regulate cardiac fibroblast activation via AT1R and TGF-β1.Exp Cell Res,2012,318(1):75-84.
9 Xu Z,Wang S,Wu M,et al.TGF-β1 and HGF protein secretion by esophageal squamous epithelial cellsand stromal fibroblasts in oesophageal carcinogenesis.Oncol Lett,2013,6(2):401-406.
10 Agajanian M,Campeau A,Hoover M,et al.Peakl acts as a molecular switch to regulate context-dependent TGF-βresponses in breast cancer.Plo S One,2015,10(8):e0135748.
11 Chen N,Geng Q,Zheng J,et al.Suppression of the TGF-β/Smad signaling pathway and inhibition of hepatic stellate cell proliferation play a role in the hepatoprotective effects of curcumin against alcoholinduced hepatic fibrosis.Int J Mol Med,2014,34(4):1110-1116.
12 Zhang Y,Yu Z,Xiao Q,et al.Expression of BAMBI and itscombination with Smad7 correlates with tumor invasion and poorprognosis in gastric cancer.Tumor Biol,2014,35(7):7047-7056.
13 Guido C,Whitaker-Menezes D,Capparelli C,et al.Metabolic reprogramming of cancer-associated fibroblasts by TGF-βdrives tumor growth:connecting TGF-βsignaling with“Warburg-like”cancer metabolism and L-lactate production.Cell Cycle,2012,11(16):3019-3035.
2 Fitchev PP,Weislak SM,Lee C,et al.Thrombospondin-1 regulates the normal prostate in vivo through angiogenesis and TGF-βactivation.Lab Invest,2010,90(7):1078-1090.
3 Zhao B,Chen YG.Regulation of TGF-βsignal transduction.Scientifica(Cairo),2014,2014:874065.
4蔡传宝,陶学金,朱声荣,等.TGF-β1、Smad4和Cyclin D1在口腔鳞癌中的表达和意义.口腔医学研究,2007,23(1):14-16.
5 Cheung DY,Kim JK.Perspectives of the stomach cancer treat-ment:the introduction of molecular targeted therapy and the hope for cure.Korean J Gastroenterol,2013,61(3):117-127.
6张晓金,归绥琪.宫颈癌发病机制的研究进展.中国妇幼健康研究,2008,19(1):56-59.
7 Katz LH,Likhter M,Jogunoori W,et al.TGF-βsignaling in liver and gastrointestinal cancers.Cancer Lett.2016,379(2):166-172.
8 Galie PA,Russell MW,Westfall MV,et al.Interstitial fluid flow and cyclic strain differentially regulate cardiac fibroblast activation via AT1R and TGF-β1.Exp Cell Res,2012,318(1):75-84.
9 Xu Z,Wang S,Wu M,et al.TGF-β1 and HGF protein secretion by esophageal squamous epithelial cellsand stromal fibroblasts in oesophageal carcinogenesis.Oncol Lett,2013,6(2):401-406.
10 Agajanian M,Campeau A,Hoover M,et al.Peakl acts as a molecular switch to regulate context-dependent TGF-βresponses in breast cancer.Plo S One,2015,10(8):e0135748.
11 Chen N,Geng Q,Zheng J,et al.Suppression of the TGF-β/Smad signaling pathway and inhibition of hepatic stellate cell proliferation play a role in the hepatoprotective effects of curcumin against alcoholinduced hepatic fibrosis.Int J Mol Med,2014,34(4):1110-1116.
12 Zhang Y,Yu Z,Xiao Q,et al.Expression of BAMBI and itscombination with Smad7 correlates with tumor invasion and poorprognosis in gastric cancer.Tumor Biol,2014,35(7):7047-7056.
13 Guido C,Whitaker-Menezes D,Capparelli C,et al.Metabolic reprogramming of cancer-associated fibroblasts by TGF-βdrives tumor growth:connecting TGF-βsignaling with“Warburg-like”cancer metabolism and L-lactate production.Cell Cycle,2012,11(16):3019-3035.