全国中药资源普查丹参药材农药残留检测
|
摘要
目的:了解全国范围内丹参农药残留现状,并为制定丹参农残含量标准提供数据基础。方法:建立检测144种农药残留的气相色谱-串联质谱法和85种农药残留的液相色谱-串联质谱法,测定全国第四次中药资源普查所收集的来源于安徽、山西、河南、河北及四川省的40批丹参样品中农药残留量,并根据2015年版《中国药典》一部丹参项下含量测定的规定对其丹参酮类和丹酚酸B成分进行含量测定,应用SAS 8.2软件分析研究丹参农药残留量与丹参酮类、丹酚酸B成分含量的相关性。结果:40批丹参样品中有20批检测出有农药残留,均为非禁用或限用农药。其中19批检出草克净/嗪草酮,3批检出氟乐灵,8批丹参样品丹参酮类和丹酚酸B成分含量不符合2015年版《中国药典》规定;草克净/嗪草酮的残留量与丹参酮类、丹酚酸B均没有相关性。结论:丹参药材栽培过程中存在非常普遍的农药使用情况,使用地区广泛,农药的种类趋于一致。草克净/嗪草酮的使用对丹参中丹参酮类、丹酚酸B的生成和代谢没有影响。该研究为全国范围内丹参农药残留现状及为丹参农残含量标准制定提供了数据支撑和科学依据。
Objective: To study the current situations of pesticide residues of Salviae Miltiorrhizae Radix et Rhizoma in China,in order to further provide data base for formulating the pesticide residues standard of Salviae Miltiorrhizae Radix et Rhizoma. Method: A gas chromatography tandem mass spectrometriy(GC-MS/MS) for detecting 144 kinds of pesticide residues and a ultra-high liquid chromatography tandem mass spectrometry(UPLCMS/MS) method for detecting 85 kinds of pesticide residues were established,in order to determine the pesticide residues of the 40 batches of samples collected from Anhui,Shanxi,Henan,Hebei and Sichuan provinces during the fourth national survey on traditional Chinese medicine resources on the basis of the China Pharmacopoeia(2015 edition). SAS 8. 2 software was applied in a correlation analysis. Result: Pesticide residues were detected in 20 batches, all of the pesticides were not banned or restricted pesticides. Metribuzin was detected in 19 batches,and trifluralin was detected in 3 batches. A total of 8 batches of samples of content did not reach the requirements of China Pharmacopoeia(2015 edition). Metribuzin,tanshinones and salvianolic acid B had not the correction. Conclusion: In the process of the cultivation of Salviae Miltiorrhizae Radix et Rhizoma,pesticides is commonly used. The use of oxalazine/azine has no impact on the production and metabolism of danshenone and danfolic acid B. This study on the current situations of pesticide residues of Salviae Miltiorrhizae Radix et Rhizoma in China provides data support and scientific basis for formulating the pesticide residues standard of Salviae Miltiorrhizae Radix et Rhizoma.
Objective: To study the current situations of pesticide residues of Salviae Miltiorrhizae Radix et Rhizoma in China,in order to further provide data base for formulating the pesticide residues standard of Salviae Miltiorrhizae Radix et Rhizoma. Method: A gas chromatography tandem mass spectrometriy(GC-MS/MS) for detecting 144 kinds of pesticide residues and a ultra-high liquid chromatography tandem mass spectrometry(UPLCMS/MS) method for detecting 85 kinds of pesticide residues were established,in order to determine the pesticide residues of the 40 batches of samples collected from Anhui,Shanxi,Henan,Hebei and Sichuan provinces during the fourth national survey on traditional Chinese medicine resources on the basis of the China Pharmacopoeia(2015 edition). SAS 8. 2 software was applied in a correlation analysis. Result: Pesticide residues were detected in 20 batches, all of the pesticides were not banned or restricted pesticides. Metribuzin was detected in 19 batches,and trifluralin was detected in 3 batches. A total of 8 batches of samples of content did not reach the requirements of China Pharmacopoeia(2015 edition). Metribuzin,tanshinones and salvianolic acid B had not the correction. Conclusion: In the process of the cultivation of Salviae Miltiorrhizae Radix et Rhizoma,pesticides is commonly used. The use of oxalazine/azine has no impact on the production and metabolism of danshenone and danfolic acid B. This study on the current situations of pesticide residues of Salviae Miltiorrhizae Radix et Rhizoma in China provides data support and scientific basis for formulating the pesticide residues standard of Salviae Miltiorrhizae Radix et Rhizoma.
引文
[1]万益群,鄢爱平,谢明勇.中草药中有机氯农药和拟除虫菊酯农药残留量的测定[J].分析化学,2005,33(5):614-618.
[2]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:76-77.
[3]张兴国,王义明,罗国安,等.丹参品种资源特性的研究[J].中草药,2002,33(8):742-747.
[4]郭宝林,林生,冯毓秀,等.丹参主要居群的遗传关系及药材道地性的初步研究[J].中草药,2002,33(12):1113-1116.
[5]李力,娄子洋,陈万生,等.不同产地丹参中3种丹参酮含量变异[J].第二军医大学学报,2000,21(8):753-755.
[6]王学勇,周晓丽,黄璐琦,等.本草文献记载5个道地产区丹参HPLC特征图谱比较研究[J].北京中医药大学学报,2011,34(6):405-408.
[7]陈勇,张玲,王世敏.丹参水溶性成分的电喷雾质谱行为及其特征图谱的初步研究[J].分析化学,2004,32(11):1485-1488.
[8]韩凤梅,张玲,陈怀侠,等.丹参脂溶性成分的ESIMS行为及其特征图谱研究[J].中草药,2006,37(1):122-125.
[9]牛敏,刘红燕,刘谦,等.4个栽培年限丹参颜色与9种活性成分含量的相关性[J].中成药,2017,39(1):131-135.
[10]宋慧.原子吸收法对丹参中的铅和镉含量分析[J].数理医药学杂志,2016,29(10):1523-1524.
[11]庞秀清,郑林,刘童,等.原子吸收光谱法测定丹参药材中铅、镉、铜、砷、汞的含量[J].贵阳医学院学报,2011,36(6):558-560,564.
[12]何平,孙巍,孙玉侠,等.微波消解-ICP-MS法测定丹参和三七药材中18种重金属元素[J].中成药,2011,33(12):2110-2113.
[13]赵爱红,王建华,宋志刚,等.ICP-AES法测定丹参中的镉和铅[J].光谱学与光谱分析,2006,26(11):2137-2139.
[14]赖志辉,徐晓铭,管艳艳,等.微波消解-ICP-AES法测定市售丹参中的微量元素[J].广西大学学报:自然科学版,2010,35(3):497-501.
[15]韩建平.GC-MS测定丹参中有机氯、有机磷农药残留的研究[D].天津:天津大学,2007.
[16]张姗姗.丹参中农药残留、黄曲霉毒素含量测定及其抗菌活性评价研究[D].锦州:锦州医科大学,2017.
[17]陈溪,崔晗,董振霖,等.大米中193种农药多残留检测方法的研究[J].沈阳农业大学学报,2015,46(5):603-607.
[18]陈红平,刘新,汪庆华,等.气相色谱-串联质谱法测定茶叶中88种农药残留量[J].色谱,2011,29(5):409-416.
[19]李记明,司合芸,于英,等.葡萄农药残留及其对葡萄酒酿造的影响[J].中国农业科学,2012,45(4):743-751.
[20]王莹,金红宇,魏赫,等.花类、果实类中药材中禁限用及常用农药多残留检测方法的建立[J].中国药学杂志,2016,51(5):404-412.
[21]何丽君,陈豪,翁淑琴,等.分散固相萃取技术结合超高效液相色谱-高分辨质谱法测定蜂蜜中三嗪类农药残留[J].中国实验方剂学杂志,2016,22(22):71-76.
[22]黄蘅,陈学松,王丽丽,等.ASE-改良Qu ECh ERS-GCMS/MS测定穿心莲中76种农药残留[J].中国实验方剂学杂志,2016,22(20):72-76.
[23]赖青海,王琳琳,石垚,等.Qu ECh ERS结合气相色谱-质谱快速测定人参提取物中25种农药残留[J].中国实验方剂学杂志,2015,21(11):55-60.
[2]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:76-77.
[3]张兴国,王义明,罗国安,等.丹参品种资源特性的研究[J].中草药,2002,33(8):742-747.
[4]郭宝林,林生,冯毓秀,等.丹参主要居群的遗传关系及药材道地性的初步研究[J].中草药,2002,33(12):1113-1116.
[5]李力,娄子洋,陈万生,等.不同产地丹参中3种丹参酮含量变异[J].第二军医大学学报,2000,21(8):753-755.
[6]王学勇,周晓丽,黄璐琦,等.本草文献记载5个道地产区丹参HPLC特征图谱比较研究[J].北京中医药大学学报,2011,34(6):405-408.
[7]陈勇,张玲,王世敏.丹参水溶性成分的电喷雾质谱行为及其特征图谱的初步研究[J].分析化学,2004,32(11):1485-1488.
[8]韩凤梅,张玲,陈怀侠,等.丹参脂溶性成分的ESIMS行为及其特征图谱研究[J].中草药,2006,37(1):122-125.
[9]牛敏,刘红燕,刘谦,等.4个栽培年限丹参颜色与9种活性成分含量的相关性[J].中成药,2017,39(1):131-135.
[10]宋慧.原子吸收法对丹参中的铅和镉含量分析[J].数理医药学杂志,2016,29(10):1523-1524.
[11]庞秀清,郑林,刘童,等.原子吸收光谱法测定丹参药材中铅、镉、铜、砷、汞的含量[J].贵阳医学院学报,2011,36(6):558-560,564.
[12]何平,孙巍,孙玉侠,等.微波消解-ICP-MS法测定丹参和三七药材中18种重金属元素[J].中成药,2011,33(12):2110-2113.
[13]赵爱红,王建华,宋志刚,等.ICP-AES法测定丹参中的镉和铅[J].光谱学与光谱分析,2006,26(11):2137-2139.
[14]赖志辉,徐晓铭,管艳艳,等.微波消解-ICP-AES法测定市售丹参中的微量元素[J].广西大学学报:自然科学版,2010,35(3):497-501.
[15]韩建平.GC-MS测定丹参中有机氯、有机磷农药残留的研究[D].天津:天津大学,2007.
[16]张姗姗.丹参中农药残留、黄曲霉毒素含量测定及其抗菌活性评价研究[D].锦州:锦州医科大学,2017.
[17]陈溪,崔晗,董振霖,等.大米中193种农药多残留检测方法的研究[J].沈阳农业大学学报,2015,46(5):603-607.
[18]陈红平,刘新,汪庆华,等.气相色谱-串联质谱法测定茶叶中88种农药残留量[J].色谱,2011,29(5):409-416.
[19]李记明,司合芸,于英,等.葡萄农药残留及其对葡萄酒酿造的影响[J].中国农业科学,2012,45(4):743-751.
[20]王莹,金红宇,魏赫,等.花类、果实类中药材中禁限用及常用农药多残留检测方法的建立[J].中国药学杂志,2016,51(5):404-412.
[21]何丽君,陈豪,翁淑琴,等.分散固相萃取技术结合超高效液相色谱-高分辨质谱法测定蜂蜜中三嗪类农药残留[J].中国实验方剂学杂志,2016,22(22):71-76.
[22]黄蘅,陈学松,王丽丽,等.ASE-改良Qu ECh ERS-GCMS/MS测定穿心莲中76种农药残留[J].中国实验方剂学杂志,2016,22(20):72-76.
[23]赖青海,王琳琳,石垚,等.Qu ECh ERS结合气相色谱-质谱快速测定人参提取物中25种农药残留[J].中国实验方剂学杂志,2015,21(11):55-60.