葛黄颗粒乙醇渗漉工艺研究
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摘要
目的优选葛黄颗粒乙醇渗漉工艺。方法以乙醇提取物中有效成分鸢尾苷含量为指标,通过单因素试验比较乙醇渗漉法、乙醇超声法和乙醇回流法,确定葛黄颗粒中醇提药材的提取方法;运用高效液相色谱法,使用正交设计法,优选葛黄颗粒乙醇提取工艺。结果采用乙醇渗漉法提取,最佳工艺为:8倍量60%乙醇,浸泡12h,流速为1m L·min-1。结论采用该渗漉工艺提取,葛黄颗粒乙醇提取物中鸢尾苷的收率较高且稳定。
Objective:To optimize the process of ethanol percolation in Gehuang Granule.Methods:By taking the content of tectoridin in the ethanol extract as an index and by single factor test,ethanol percolation method,ethanol ultrasonic method and ethanol reflux method were compared to determine the extraction method of alcohol extraction medicinal material in Gezhong granule.By using HPLC and orthogonal design method,the ethanol extraction process of Gezhong Granule was optimized.Results:The best extraction process was ethanol percolation method.The optimum conditions were as follows,8 times the amount of 60% ethanol,soaking for 12 hours,with 1 m L·min-1 of flow rate.Conclusion:According to the percolation process,the yield of tectoridin of ethanol percolation in Gehuang Granule is high and stable.
Objective:To optimize the process of ethanol percolation in Gehuang Granule.Methods:By taking the content of tectoridin in the ethanol extract as an index and by single factor test,ethanol percolation method,ethanol ultrasonic method and ethanol reflux method were compared to determine the extraction method of alcohol extraction medicinal material in Gezhong granule.By using HPLC and orthogonal design method,the ethanol extraction process of Gezhong Granule was optimized.Results:The best extraction process was ethanol percolation method.The optimum conditions were as follows,8 times the amount of 60% ethanol,soaking for 12 hours,with 1 m L·min-1 of flow rate.Conclusion:According to the percolation process,the yield of tectoridin of ethanol percolation in Gehuang Granule is high and stable.
引文
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[2]姚美村,廖祎婷,袁月梅,等.不同葛花提取物对乙醇致小鼠肝损伤保护作用的比较研究[J].时珍国医国药,2011,22(4):883-885.
[3]曾明,邸晓辉.葛根及葛花对酒精代谢的研究[J].医药论坛杂志,2010,31(23):203-205.
[4]尹俊亭,仲英,孙敬勇,等.葛花化学成分的研究(Ⅰ)[J].中草药,2006,37(3):350-351.
[5]华卡,黄宇.高效液相色谱法测定不同产地葛花中葛花苷的含量[J].中南药学,2012,10(6):435-437.
[6]陈玉兰,蒲清荣,魏嵋,等.HPLC法测定葛花药材中鸢尾苷的含量[J].亚太传统医药,2016,12(156):22-23.
[7]刘立辉,李卫民,高英.HPLC同时测定野葛花中鸢尾黄素-7-O-木糖葡萄糖苷、鸢尾苷、鸢尾苷元的含量[J].中国中药杂志,2010,35(17):2308-2311.
[8]赵剑,蒲清荣,陈玉兰,等.茵陈四苓颗粒乙醇提取工艺优选[J].中国实验方剂学杂志,2013,19(22):31-34.