基于药性组合的活血化瘀中药性效关系研究
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摘要
中药功效是药物作用于人体后产生的治疗作用,中药药性是功效作用的进一步概括,功效与药性之间存在必然的内在关系。该文提出以药性组合为基本单位,通过"药性组合-中药作用靶标-生物网络模块-功效"的研究模式,在整体层次上发现药性组合与中药功效、药效间的关系。研究表明,温辛肝组合通过参与脂质代谢、血液凝固、血小板活化、血红素氧化、血小板脱粒、细胞凋亡、炎症反应等生命过程来达到抗肿瘤、抗血栓、抗心肌缺血和抗炎等药理药效,从而发挥其活血行气的功效。
The efficacy of traditional Chinese medicine is the therapeutic effect of the drug on the body. The nature of traditional Chinese medicine is a further generalization of the effect of efficacy,and there is an intrinsic relationship between efficacy and nature of traditional Chinese medicine. In this study,the nature-effect relationship is found on the whole level,through the research mode of " nature combination-targets of traditional Chinese medicine-modules of protein interaction network-efficiency". The results showed that the warm-pungent-liver protein interaction network mainly participated in lipid catabolic process,blood coagulation,platelet activation,heme oxidation,platelet degranulation,apoptotic process,acute inflammatory response to exert the effect of anti-tumor,antithrombotic,anti-myocardial ischemia and anti-inflammatory.
The efficacy of traditional Chinese medicine is the therapeutic effect of the drug on the body. The nature of traditional Chinese medicine is a further generalization of the effect of efficacy,and there is an intrinsic relationship between efficacy and nature of traditional Chinese medicine. In this study,the nature-effect relationship is found on the whole level,through the research mode of " nature combination-targets of traditional Chinese medicine-modules of protein interaction network-efficiency". The results showed that the warm-pungent-liver protein interaction network mainly participated in lipid catabolic process,blood coagulation,platelet activation,heme oxidation,platelet degranulation,apoptotic process,acute inflammatory response to exert the effect of anti-tumor,antithrombotic,anti-myocardial ischemia and anti-inflammatory.
引文
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[38] Lievens D,Von H P. Platelets in atherosclerosis[J]. Thrombhaemostasis,2011,106(5):827.
[39]庞兴学,王显.血栓形成的过程与机制研究进展[J].医学综述,2011,17(11):1613.
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[42]李家明,赵永海,钟国琛,等.阿魏酸衍生物的合成及抗血小板聚集活性[J].药学学报,2011,28(3):305.
[43]李敏,林俊.细胞凋亡途径及其机制[J].国际妇产科学杂志,2014,41(2):103.
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[45]胡昆,徐华金,辛文群,等.刺芒柄花素氮芥衍生物的合成及抗肿瘤活性[J].中国药科大学学报,2012,43(2):113.
[46]段佳林,奚苗苗,牟菲,等.紫铆花素通过调节AMPK/GSK-3β信号通路抑制心肌缺血再灌注损伤[J].现代生物医学进展,2017,17(14):2616.
[47]宋艳群,祝融峰,陈鹏.血红素的生理分布与调控[J].中国科学:化学,2015,45(11):1194.
[48]岳黎明,畅辉,杨志偲.川芎嗪对冠状动脉慢血流患者血管内皮功能的影响[J].中国循证心血管医学杂志,2018,10(2):193.
[49]沈映君.中药药理学[M]. 2版.北京:人民卫生出版社,2011:565.
[50]孙建宁.中药药理学[M].北京:中国中医药出版社,2014:185.
[51]孙广仁,郑洪新.中医基础理论[M]. 3版.北京:中国中医药出版社,2012:112.
[2]于培明,田智勇,林桂涛.甘味药的药性理论及其配伍探讨[J].国医论坛,2005,20(1):48.
[3]苗明三,张玉林,杨亚蕾,等.基于中药药理作用的中药药性理论研究[J].时珍国医国药,2009,20(8):2013.
[4]王米渠,严石林,李炜弘,等.寒热性中药对SD大鼠的实验研究[J].浙江中医药大学学报,2002,26(6):43.
[5]王耘,张燕玲,郭亦然,等.应用涌现性原理研究方剂配伍规律[J].中国组织工程研究,2005,9(21):155.
[6]高冲,刘璐,胡爱菊,等.活血化瘀中药的药理作用研究进展[J].药物评价研究,2013,36(1):64.
[7]肖小河.中药药性研究概论[J].中草药,2008,39(4):481.
[8]陈素红,吕圭源.“性、味结合归经”层面研究中药药性[J].中药药理与临床,2008,24(4):58.
[9]王晓艳.整体观下的辨证论治和辨病论治关系论[J].中医临床研究,2017,9(28):43.
[10]钟赣生.中药学[M]. 4版.北京:中国中医药出版社,2016:21.
[11]肖斌,王耘,郭维嘉,等.中药药性组合及其与功效的关系研究[J].世界科学技术——中医药现代化,2010,12(6):902.
[12]王耘,张燕玲,史新元,等.基于药性组合的中药性效规律研究框架[J].世界科学技术——中医药现代化,2012,14(4):1798.
[13]马中水.介观物理基础和近期发展几个方面的简单介绍[J].物理,2007,36(2):98.
[14]魏志成,童东,杨娟,等.基于网络药理学的沙棘总黄酮治疗心肌缺血的作用机制研究[J].中国中药杂志,2017,42(7):1238.
[15]刘楠,姜云耀,黄婷婷,等.基于网络药理学方法研究补阳还五汤治疗脑梗死的作用机制[J].中国中药杂志,2018,43(11):2190.
[16]范文涛,王倩.基于网络药理学的石菖蒲-郁金药对治疗抑郁症作用机制研究[J].中国中药杂志,2018,43(12):2607.
[17] Gaulton A,Bellis L J,Bento A P,et al. ChEMBL:a large-scale bioactivity database for drug discovery[J]. Nucleic Acids Res,2012,40:1100.
[18]杨志宏,梅超,孙晓波.降香化学成分、药理作用及药代特征的研究进展[J].中国中药杂志,2013,38(11):1679.
[19]金玉青,洪远林,李建蕊,等.川芎的化学成分及药理作用研究进展[J].中药与临床,2013,4(3):44.
[20] Hobohm U,Scharf M,Schneider R,et al. Selection of representative protein data sets[J]. Protein Sci,1992,1(3):409.
[21] Franz M,Lopes C T,Huck G,et al. Cytoscape.js:a graph theory library for visualisation and analysis[J]. Bioinformatics,2016,32(2):309.
[22]郑世超,严小英,陈菊,等.基于蛋白互作网络分析祛风湿药川乌的抗炎机制[J].中国中药杂志,2017,42(9):1747.
[23] Huo M,Wang Z,Wu D,et al. Using co-expression protein interaction network analysis to identify mechanisms of danshensu affecting patients with coronary heart disease[J]. Int J Mol Sci,2017,18(6):1298.
[24] Xia L,Su X,Shen J,et al. ANLN functions as a key candidate gene in cervical cancer as determined by integrated bioinformatic analysis[J]. Cancer Manag Res,2018,10:663.
[25] Barabasi A L,Oltvai Z N. Network biology:understanding the cell's functional organization[J]. Nat Rev Genet,2004,5(2):101.
[26]车宏安,顾基发.无标度网络及其系统科学意义[J].系统工程理论与实践,2004,24(4):11.
[27] Watts D J,Strogatz S H. Collective dynamics of small-world'networks[J]. Nature,1998,393:440.
[28] Li M,Li D,Tang Y,et al. CytoCluster:a cytoscape plugin for cluster analysis and visualization of biological networks[J]. Int J Mol Sci,2017,18(9):1880.
[29] Li M,Chen J,Wang J,et al. Modifying the DPClus algorithm for identifying protein complexes based on new topological structures[J]. BMC Bioinformatics,2008,9(1):398.
[30] Spirin V,Mirny L A. Protein complexes and functional modules in molecular networks[J]. Proc Natl Acad Sci USA,2003,100(21):12123.
[31] King A D,Przulj N,Jurisica I. Protein complex prediction via cost-based clustering[J]. Bioinformatics,2004,20(17):3013.
[32] Bader G D,Hogue C W. An automated method for finding molecular complexes in large protein interaction networks[J]. BMC Bioinformatics,2003,4(1):2.
[33]段练,刘咏梅,王阶.血瘀证中医证候模型的现状与展望[J].中医杂志,2017,58(4):346.
[34]龚勇珍,孙少卫,廖端芳.细胞炎症反应与脂质代谢的相互作用及调节[J].中国动脉硬化杂志,2017,25(6):623.
[35]于佳雪,徐亚伟,肖丹,等.游离脂肪酸与心血管疾病[J].现代生物医学进展,2013,13(10):1979.
[36]赵培,杨媛,徐扬,等.刺芒柄花素调节血脂代谢及改善动脉粥样硬化病变的实验研究[J].中国新药杂志,2009(10):925.
[37] Jiang F,Qian J,Chen S,et al. Ligustrazine improves atherosclerosis in rat via attenuation of oxidative stress[J]. Pharm Biol,2011,49(8):856.
[38] Lievens D,Von H P. Platelets in atherosclerosis[J]. Thrombhaemostasis,2011,106(5):827.
[39]庞兴学,王显.血栓形成的过程与机制研究进展[J].医学综述,2011,17(11):1613.
[40] Zhang S,Cheng J,Chen W,et al. Interactions between thrombin and natural products of Millettia nitita var. hirsutissima using capillary zone electrophoresis[J]. J Chromatogr B,2009,877(32):4107.
[41]蒋跃绒,陈可冀.川芎嗪的心脑血管药理作用及临床应用研究进展[J].中国中西医结合杂志,2013,33(5):707.
[42]李家明,赵永海,钟国琛,等.阿魏酸衍生物的合成及抗血小板聚集活性[J].药学学报,2011,28(3):305.
[43]李敏,林俊.细胞凋亡途径及其机制[J].国际妇产科学杂志,2014,41(2):103.
[44] Jia W C,Liu G,Zhang C D,et al. Formononetin attenuates hydrogen peroxide(H2O2)-induced apoptosis and NF-κB activation in RGC-5 cells[J]. Eur Rev Med Pharmacol,2014,18(15):2191.
[45]胡昆,徐华金,辛文群,等.刺芒柄花素氮芥衍生物的合成及抗肿瘤活性[J].中国药科大学学报,2012,43(2):113.
[46]段佳林,奚苗苗,牟菲,等.紫铆花素通过调节AMPK/GSK-3β信号通路抑制心肌缺血再灌注损伤[J].现代生物医学进展,2017,17(14):2616.
[47]宋艳群,祝融峰,陈鹏.血红素的生理分布与调控[J].中国科学:化学,2015,45(11):1194.
[48]岳黎明,畅辉,杨志偲.川芎嗪对冠状动脉慢血流患者血管内皮功能的影响[J].中国循证心血管医学杂志,2018,10(2):193.
[49]沈映君.中药药理学[M]. 2版.北京:人民卫生出版社,2011:565.
[50]孙建宁.中药药理学[M].北京:中国中医药出版社,2014:185.
[51]孙广仁,郑洪新.中医基础理论[M]. 3版.北京:中国中医药出版社,2012:112.