大孔树脂吸附分离S-腺苷甲硫氨酸的研究
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摘要
采用大孔树脂吸附法对S-腺苷甲硫氨酸(S-adenosylmethionine,SAM)纯化工艺进行研究。通过考察上柱流速、浓度、p H等因素对S-腺苷甲硫氨酸动态吸附的泄露曲线和树脂饱和吸附量影响,确定S-腺苷甲硫氨酸树脂动态吸附的最佳工艺条件为:上柱流速3.6 m L/min,进样料液浓度5.0 g/L,初始p H=5.0。用0.5 mol/L硫酸以流速3.6 m L/min洗脱吸附饱和树脂,产品得率为92.57%。树脂重复使用5次后其对SAM的吸附量和解吸率分别下降了13.68 mg/g和6.76%。
The purification process of S-adenosylmethionine was studied by macroporous resin adsorption. By investigating the effect of flow rate,concentration and p H value on leakage curve and saturated adsorption capacity of S-adenosylmethionine during resin dynamic adsorption,the optimum purification conditions were obtained as follows:the flow rate was 3.6 m L/min,the concentration of feed liquid was 5.0 g/L,the initial p H was5.0. The saturated resin was eluted with 0.5 mol/L sulfuric acid at a flow rate of 3.6 m L/min,and the yield of Sadenosylmethionine was 92.57 %. The adsorption and desorption properties of S-adenosylmethionine decreased by 13.68 mg/g and 6.76 % respectively after repeated use for 5 times.
The purification process of S-adenosylmethionine was studied by macroporous resin adsorption. By investigating the effect of flow rate,concentration and p H value on leakage curve and saturated adsorption capacity of S-adenosylmethionine during resin dynamic adsorption,the optimum purification conditions were obtained as follows:the flow rate was 3.6 m L/min,the concentration of feed liquid was 5.0 g/L,the initial p H was5.0. The saturated resin was eluted with 0.5 mol/L sulfuric acid at a flow rate of 3.6 m L/min,and the yield of Sadenosylmethionine was 92.57 %. The adsorption and desorption properties of S-adenosylmethionine decreased by 13.68 mg/g and 6.76 % respectively after repeated use for 5 times.
引文
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[12]陈勇.以离子交换法为核心典型胞外(米多美素)和胞内(S-腺苷甲硫氨酸)产物制备技术的而研究[D].杭州:浙江大学,2004:7-8
[13]赵昕,于一帆,苏婉莹,等.大孔树脂吸附分离黑胡萝卜红色素的研究[J].食品研究与开发,2017,38(23):38-43
[14]张洪坤.大孔树脂吸附纯化茯苓多糖工艺研究[J].食品研究与开发,2017,38(23):67-71
[15]杜瑾,张爱君,张晓青,等.大孔弱酸树脂对S-腺苷-甲硫氨酸静态吸附性能[J].食品与发酵科技,2017,53(6):17-21
[16]刘军伟,李啸晨,侯婴惠,等.大孔树脂纯化生姜多酚的研究[J].食品研究与开发,2017,38(17):35-39
[17]康家胜,方春,张静,等.AB-8型树脂对无患子皂苷的动态吸附与解吸性能[J].天然产物研究与开发,2011,23(4):709-712
[18]姚进孝.S-腺苷-L-甲硫氨酸分离纯化工艺研究[D].北京:北京化工大学,2008:41-45
[2]BOTTIGLIERI T.S-Adenosyl-L-methionine(SAMe):from the bench to the bedside-molecular basis of a pleiotrophic molecule[J].American Journal of Clinical Nutrition,2002,76(5):1151-1157
[3]LIEBER CS.S-adenosyl-L-methionine:its role in the treatment of liver disorders[J].American Journal of Clinical Nutrition,2002,76(5):1183-1187
[4]Niu W,Cao S,Yang M,et al.Enzymatic synthesis of S-adenosylmethionine using immobilized methionine adenosyltransferase variants on the 50-m M scale[J/OL].Catalysts,2017,7(8):238[2017-8-17].http://www.mdpi.com/journal/catalysts,DOI:10.3390/catal7080238
[5]CHUN LY,TAO ZH,XIN CH.Biosynthesis of S-Adenosylmethionine by Magnetically Immobilized Escherichia coli Cells Highly Expressing a Methionine Adenosyltransferase Variant[J/OL].Molecules,2017,22(8):1365[2017-8-18].http://www.mdpi.com/journal/molecules,DOI:10.3390/molecules22081365
[6]张渝杰,詹良静,张新宜,等.响应面法优化S-腺苷蛋氨酸发酵培养基的研究[J].中国抗生素杂志,2013,38(4):255-260
[7]CAO X,YANG M,XIA Y,et al.Strain improvement for enhanced production of S-adenosyl-L-methionine in Saccharomyces cerevisiae,based on ethionine-resistance and SAM synthetase activity[J].Annals of Microbiology,2012,62(4):1395-1402
[8]LI MH,MENG XM,DIAO EJ,et al.Productivity enhancement of Sadenosylmethionine in Saccharomyces cerevisiae,using n-hexadecane as oxygen vector[J].Journal of Chemical Technology&Biotechnology,2012,87(10):1379-1384
[9]HARIT R K,BALAMURALI M,MEENAKSHISUNDARAM S.Enhancing precursors availability in pichia pastoris for the overproduction of S-adenosyl-L-methionine employing molecular strategies with process tuning[J].Journal of Biotechnology,2014,188:112-121
[10]刘惠,吴坚平,林建平,等.S-腺苷-L-蛋氨酸的分离纯化及其稳定产品的制备[J].中国生化药物杂志,2002,23(6):284-287
[11]傅梦月,张建国,张继宁.重组毕赤酵母中腺苷甲硫氨酸的纯化[J].食品与发酵工业,2015,41(6):15-19
[12]陈勇.以离子交换法为核心典型胞外(米多美素)和胞内(S-腺苷甲硫氨酸)产物制备技术的而研究[D].杭州:浙江大学,2004:7-8
[13]赵昕,于一帆,苏婉莹,等.大孔树脂吸附分离黑胡萝卜红色素的研究[J].食品研究与开发,2017,38(23):38-43
[14]张洪坤.大孔树脂吸附纯化茯苓多糖工艺研究[J].食品研究与开发,2017,38(23):67-71
[15]杜瑾,张爱君,张晓青,等.大孔弱酸树脂对S-腺苷-甲硫氨酸静态吸附性能[J].食品与发酵科技,2017,53(6):17-21
[16]刘军伟,李啸晨,侯婴惠,等.大孔树脂纯化生姜多酚的研究[J].食品研究与开发,2017,38(17):35-39
[17]康家胜,方春,张静,等.AB-8型树脂对无患子皂苷的动态吸附与解吸性能[J].天然产物研究与开发,2011,23(4):709-712
[18]姚进孝.S-腺苷-L-甲硫氨酸分离纯化工艺研究[D].北京:北京化工大学,2008:41-45