蛋白质组学技术及其在中医药治疗骨质疏松症研究的应用进展
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摘要
综述近年来蛋白质组学技术的发展及其在中医药治疗骨质疏松症中的运用概况。蛋白质组学常用技术包括蛋白质分离技术、蛋白质鉴定技术及生物信息学技术。蛋白质组学技术主要用于单味中药、中药复方及针刺治疗骨质疏松症的疗效机制研究,并已取得一定的进展。认为蛋白质组学技术将在骨质疏松症的证候分型与蛋白质的相关性研究、骨质疏松早期预防和诊断与及时治疗以及中医药治疗骨质疏松症的蛋白质水平作用途径研究中具有广阔的应用前景。
The progress of proteomics technology and its application in the research of osteoporosis treated with traditional Chinese medicine was reviewed. The proteomics technology included protein separation technology,protein identification technology,and bioinformatics technology. Proteomics technology was now mainly used forthe study of therapeutic mechanism of single herb, compound herbal recipe and acupuncture in treatingosteoporosis,and certain progress has been achieved. It is indicated that proteomics technology will have broadprospects in the research of correlation of syndrome differentiation of osteoporosis with proteins,early preventionand diagnosis and treatment, and protein therapeutic mechanism of traditional Chinese medicine in treatingosteoporosis.
The progress of proteomics technology and its application in the research of osteoporosis treated with traditional Chinese medicine was reviewed. The proteomics technology included protein separation technology,protein identification technology,and bioinformatics technology. Proteomics technology was now mainly used forthe study of therapeutic mechanism of single herb, compound herbal recipe and acupuncture in treatingosteoporosis,and certain progress has been achieved. It is indicated that proteomics technology will have broadprospects in the research of correlation of syndrome differentiation of osteoporosis with proteins,early preventionand diagnosis and treatment, and protein therapeutic mechanism of traditional Chinese medicine in treatingosteoporosis.
引文
[1]中华医学会骨质疏松和骨矿盐疾病分会.原发性骨质疏松症诊疗指南(2017)[J].中华骨质疏松和骨矿盐疾病杂志,2017,10(5):413.
[2] Xiong Q,Tang P,Gao Y,et al. Proteomic analysis of estrogenmediated signal transduction in osteoclasts formation[J]. Biomed Res Int,2015,2015:596789.
[3]王新贤,殷海波,姜泉,等.蛋白质组学在中医证候学研究中的应用进展[J].世界中医药,2017,12(8):1965.
[4]王寿勇,马琳玉,王颖,等.大鼠体外循环前后血浆蛋白质差异性表达[J].重庆医学,2015,44(10):1311.
[5]曾建春,樊粤光,刘建仁,等.杜仲含药血清诱导骨髓间充质干细胞定向分化蛋白质组学研究[J].时珍国医国药,2010,21(2):274.
[6]薛黎明.基于蛋白质组学淫羊藿苷防治骨质疏松作用机理及药对“淫羊藿仙茅”配伍机制研究[D].上海:第二军医大学,2012.
[7]招文华,沈耿杨,任辉,等.骨碎补活性单体成分调控骨质疏松症相关信号通路的研究进展[J].中国骨质疏松杂志,2017,23(1):122.
[8]卢严方,林贯川,刘倩倩,等.六味地黄丸对绝经期肾阴虚骨质疏松症的基因表达调控数据分析[J].中国骨质疏松杂志,2017,23(3):350.
[9]陈娟,谢丽华,李生强,等. JAK/STAT通路介导的六味地黄丸对绝经后骨质疏松症肾阴虚证的免疫调节作用[J].中华中医药杂志,2017,32(4):1747.
[10]刘志文,刘波,吴琪,等.二仙汤对去卵巢骨质疏松大鼠股骨蛋白质组的影响[J].中国中药杂志,2017,42(13):2558.
[11]张颖,李涢,张杰,等.基于蛋白质组学方法研究左归丸治疗去卵巢所致骨质疏松症大鼠的作用机制[J].河北中医,2016,38(7):1024.
[12]杨军,张小莉,莫新民.基于蛋白质组学壮骨止痛方治疗骨质疏松症的作用机理研究[J].北京中医药大学学报,2013,36(12):817.
[13]杨军,莫新民,李劲平.壮骨止痛方治疗大鼠去卵巢骨质疏松症的蛋白质组学分析[J].中国中医基础医学杂志,2012,18(2):166.
[14]潘小燕,王鸿度,岳荣超,等.电针足少阳经穴对去卵巢大鼠骨质疏松症骨组织OPG、RANKL及CBFα1 mRNA表达的影响[J].中华中医药杂志,2017,32(11):5132.
[15]王亚军,浪万英,宋亚文,等.针刺血清对成骨细胞OPG、RANKL mRNA表达的影响[J].中国骨质疏松杂志,2017,23(10):1334.
[16]秦玮,纪峰,林莺,等.电针命门穴对去卵巢骨质疏松大鼠下丘脑骨形成蛋白BMP-2及其信号转导蛋白Smad1/5表达的影响[J].时珍国医国药,2016,27(6):1530.
[17]谢兵,袁彬彬,李颖,等.骨质疏松症与表观遗传学[J].中国骨质疏松杂志,2016,22(7):918.
[18]张志强,李春雯,潘定权,等.蛋白质组学在骨质疏松中的应用[J].中华中医药学刊,2014,32(6):1316.
[19]曾海涛,吴飞,何启艇,等.骨质疏松症的全基因组关联研究进展[J].中国骨质疏松杂志,2016,22(12):1623.
[2] Xiong Q,Tang P,Gao Y,et al. Proteomic analysis of estrogenmediated signal transduction in osteoclasts formation[J]. Biomed Res Int,2015,2015:596789.
[3]王新贤,殷海波,姜泉,等.蛋白质组学在中医证候学研究中的应用进展[J].世界中医药,2017,12(8):1965.
[4]王寿勇,马琳玉,王颖,等.大鼠体外循环前后血浆蛋白质差异性表达[J].重庆医学,2015,44(10):1311.
[5]曾建春,樊粤光,刘建仁,等.杜仲含药血清诱导骨髓间充质干细胞定向分化蛋白质组学研究[J].时珍国医国药,2010,21(2):274.
[6]薛黎明.基于蛋白质组学淫羊藿苷防治骨质疏松作用机理及药对“淫羊藿仙茅”配伍机制研究[D].上海:第二军医大学,2012.
[7]招文华,沈耿杨,任辉,等.骨碎补活性单体成分调控骨质疏松症相关信号通路的研究进展[J].中国骨质疏松杂志,2017,23(1):122.
[8]卢严方,林贯川,刘倩倩,等.六味地黄丸对绝经期肾阴虚骨质疏松症的基因表达调控数据分析[J].中国骨质疏松杂志,2017,23(3):350.
[9]陈娟,谢丽华,李生强,等. JAK/STAT通路介导的六味地黄丸对绝经后骨质疏松症肾阴虚证的免疫调节作用[J].中华中医药杂志,2017,32(4):1747.
[10]刘志文,刘波,吴琪,等.二仙汤对去卵巢骨质疏松大鼠股骨蛋白质组的影响[J].中国中药杂志,2017,42(13):2558.
[11]张颖,李涢,张杰,等.基于蛋白质组学方法研究左归丸治疗去卵巢所致骨质疏松症大鼠的作用机制[J].河北中医,2016,38(7):1024.
[12]杨军,张小莉,莫新民.基于蛋白质组学壮骨止痛方治疗骨质疏松症的作用机理研究[J].北京中医药大学学报,2013,36(12):817.
[13]杨军,莫新民,李劲平.壮骨止痛方治疗大鼠去卵巢骨质疏松症的蛋白质组学分析[J].中国中医基础医学杂志,2012,18(2):166.
[14]潘小燕,王鸿度,岳荣超,等.电针足少阳经穴对去卵巢大鼠骨质疏松症骨组织OPG、RANKL及CBFα1 mRNA表达的影响[J].中华中医药杂志,2017,32(11):5132.
[15]王亚军,浪万英,宋亚文,等.针刺血清对成骨细胞OPG、RANKL mRNA表达的影响[J].中国骨质疏松杂志,2017,23(10):1334.
[16]秦玮,纪峰,林莺,等.电针命门穴对去卵巢骨质疏松大鼠下丘脑骨形成蛋白BMP-2及其信号转导蛋白Smad1/5表达的影响[J].时珍国医国药,2016,27(6):1530.
[17]谢兵,袁彬彬,李颖,等.骨质疏松症与表观遗传学[J].中国骨质疏松杂志,2016,22(7):918.
[18]张志强,李春雯,潘定权,等.蛋白质组学在骨质疏松中的应用[J].中华中医药学刊,2014,32(6):1316.
[19]曾海涛,吴飞,何启艇,等.骨质疏松症的全基因组关联研究进展[J].中国骨质疏松杂志,2016,22(12):1623.