斑茅割手密复合体杂交利用过程野生特异基因遗传分析
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摘要
揭示斑茅割手密复合体在杂交利用过程中的斑茅、割手密野生特异基因在各世代的遗传规律,为利用斑割复合体创制甘蔗育种新亲本提供理论依据。利用AFLP-PCR分子标记结合毛细管电泳技术对斑割复合体在杂交利用过程中的斑茅、割手密野生特异基因在各世代的传递动态进行分析,并研究它们之间的遗传关系。29对AFLP引物组合共扩增出3695个位点,多态性比例为97.89%。斑茅和割手密对斑割复合体的遗传贡献率分别为43.96%和56.04%。斑茅特异位点在F1、BC1和BC2 3个世代的平均遗传率分别为8.25%、1.90%和0.63%,割手密特异位点在F1、BC1和BC2 3个世代的平均遗传率分别为16.98%、2.40%和0.21%,特异遗传物质均呈逐代减少趋势。比较不同世代甘蔗栽培种亲本遗传到后代的特异位点比率,F1的GT02-761特异位点比率最高,BC1的GT05-2743特异位点比例平均高达92.75%,BC2的ROC23遗传率最低,为49.09%,FN39遗传率最高,达94.32%。聚类分析结果表明斑割复合体偏向父本遗传,斑割复合体杂交后代偏向甘蔗栽培种遗传,与分子遗传关系分析结果一致。研究表明,经过3代的遗传重组,斑割复合体后代的遗传物质与斑割复合体相比已发生了很大的改变;研究明确了斑茅、割手密2个亲本在3个世代的遗传贡献规律,为进一步的杂交选育提供理论支持。
The inheritance regularity of wild specific loci for Erianthus arundinaceus(Retz.)Jeswiet and Saccharum spontaneum L. from intergeneric hybrid complex(E. arundinaceus×S. spontaneum)inheriting to generations was investigated,in order to provide theoretical basis for exploring new sugarcane parents. AFLP-PCR molecular markers combined with capillary electrophoresis were used to analyze the transmission dynamics of wild speci?c loci over generations and also clarify the genetic relationship among these materials. A total of 3695 loci were amplified from 29 AFLP primer combinations,with a polymorphic ratio of 97.89%. The genetic fragments derived from E. arundinaceus(Retz.)Jeswiet and S. spontaneum L.were 43.96% and 56.04% respectively. The average heritability of E. arundinaceus(Retz.)Jeswiet in F1,BC1 and BC2 were 8.25%,1.9% and 0.63%,respectively,and the average heritability of S. spontaneum L.in F1,BC1 and BC2 was 16.98%,2.4% and 0.21% respectively. Both indicated a reduction of foreign speci?c genetic loci over generations. By analyzing the ratios of sugarcane speci?c loci that remained in different generations,the genetic information of GT02-761 has been ranked ?rst in F1. The average percentage of special loci for GT05-2743 inheriting to BC1 was 92.75%. The heritability for ROC23 inheriting to BC2 was the lowest,and the heritability for FN39 was the highest,up to 94.32%.The cluster analysis showed that the intergeneric hybrid complex was inherited more from male parent and the progeny from the intergeneric hybrid complex were inherited more from the sugarcane(Saccharum spp.),which was consistent with the analysis of molecular genetic relationship. The results showed that the genetic loci for the hybrids from intergeneric hybrid complex had changed a lot compared with that of intergeneric hybrid complex in the process of genetic recombination for three generations. Thus,this work analyzed the inheritance regularity for two parents inheriting to three generations,and the outcome might provide suggestions for future breeding.
The inheritance regularity of wild specific loci for Erianthus arundinaceus(Retz.)Jeswiet and Saccharum spontaneum L. from intergeneric hybrid complex(E. arundinaceus×S. spontaneum)inheriting to generations was investigated,in order to provide theoretical basis for exploring new sugarcane parents. AFLP-PCR molecular markers combined with capillary electrophoresis were used to analyze the transmission dynamics of wild speci?c loci over generations and also clarify the genetic relationship among these materials. A total of 3695 loci were amplified from 29 AFLP primer combinations,with a polymorphic ratio of 97.89%. The genetic fragments derived from E. arundinaceus(Retz.)Jeswiet and S. spontaneum L.were 43.96% and 56.04% respectively. The average heritability of E. arundinaceus(Retz.)Jeswiet in F1,BC1 and BC2 were 8.25%,1.9% and 0.63%,respectively,and the average heritability of S. spontaneum L.in F1,BC1 and BC2 was 16.98%,2.4% and 0.21% respectively. Both indicated a reduction of foreign speci?c genetic loci over generations. By analyzing the ratios of sugarcane speci?c loci that remained in different generations,the genetic information of GT02-761 has been ranked ?rst in F1. The average percentage of special loci for GT05-2743 inheriting to BC1 was 92.75%. The heritability for ROC23 inheriting to BC2 was the lowest,and the heritability for FN39 was the highest,up to 94.32%.The cluster analysis showed that the intergeneric hybrid complex was inherited more from male parent and the progeny from the intergeneric hybrid complex were inherited more from the sugarcane(Saccharum spp.),which was consistent with the analysis of molecular genetic relationship. The results showed that the genetic loci for the hybrids from intergeneric hybrid complex had changed a lot compared with that of intergeneric hybrid complex in the process of genetic recombination for three generations. Thus,this work analyzed the inheritance regularity for two parents inheriting to three generations,and the outcome might provide suggestions for future breeding.
引文
[1]高云,李树君,矫健,秦学敏,范堃·广西糖料蔗产业发展路径分析·世界农业,2016(9):237-343Gao Y, Li S J, Jiao J, Qin X M, Fan K. Analysis on the development path of sugarcane industry in Guangxi-World Agriculture, 2016(9):237-343
[2]黄永吉,吴嘉云,刘少谋,邓祖湖,符成,林彦铨,李奇伟,黄忠兴,陈如凯·基于GISH的甘蔗与斑茅F1染色体遗传与核型分析·植物遗传资源学报,2014, 15(2):394-398Huang Y J, Wu J Y, Liu S M, Deng Z H, Fu C, Lin Y Q, Li Q W, Huang Z X, Chen R K. Chromosome genetic and karyotype analysis of F1 hybrids between Saccharum officinarum and Erianthus arundinaceus based on GISH-Journal of Plant Genetic Resources, 2014, 15(2):394-398
[3]高轶静,方锋学,刘昔辉,张荣华,宋焕忠,杨荣仲,罗霆,段维兴,游建华,张革民·甘蔗与斑茅割手密复合体杂交后代的分子标记鉴定·植物遗传资源学报,2012, 13(5):912-916Gao Y J, Fang F X, Liu X H, Zhang R H, Song H Z, Yang R Z, Luo T, Duan W X, You J H, Zhang G M. Identification of progeny from crosses between sugarcane(Saccharum spp.)and intergeneric hybrid complex(Erianthus arundinaceus x Saccharum spontaneum)with molecular markers. Journal of Plant GeneticResources,2012, 13(5):912-916
[4]中华人民共和国农业部·中国作物遗传资源·北京:中国农业出版社,1998:499-518Ministry of agriculture of the People's Republic of China.Crop genetic resources in China. Beijing:China Agricultural Publishs,1998:499-518
[5]陈辉,范源洪,史宪伟,蔡青,张明,张亚平.甘蔗细茎野生种(Saccharunsponta-neum L.)的遗传多样性和系统演化研究·作物学报,2001,27(5):645-652Chen H, Fan Y H, Shi X W, Cai Q, Zhang M, Zhang Y P. Research on genetic diversity and systemic evolution in Saccharum spontaneum L. Acta Agronomica Sinica, 2001,27(5):645-652
[6]陈如凯·现代甘蔗育种的理论与实践·北京:中国农业出版社,2003:11-12Chen R K.Theory and practice of modern sugarcane breeding.Beijing:China Agricultural Publishs, 2003:11-12
[7]王先宏,郭超,娄红波,李富生,何丽莲,何顺长,唐荣平,杨清辉·不同基因型割手密无性系的核型分析·植物遗传资源学报,2014, 15(4):912-917Wang X H,Guo C,Lou H B,Li F S,He L L,He S C.Tang R P, Yang Q H-Karyotype analysis of different Saccharum spontaneum genotype clones. Journal of Plant Genetic Resources, 2014, 15(4):912-917
[8]Lekshmi M, Pazhany A S, Sobhakumari V P, Premachandran N M-Nuclear and cytoplasmic contributions from Erianthus arundinaceus(Retz.)Jeswiet in a sugarcane hybrid clone confirmed through genomic in situ hybridization and cytoplasmic DNA polymorphism. Genetic Resources and Crop Evolution, 2017, 64:1553-1560
[9]张革民,刘昔辉,方锋学,张荣华,宋焕忠,方位宽,游建华,刘海彬·斑茅割手密复合体创制及其与甘蔗杂交F1的染色体计数和形态表现·2009年中国作物学会学术年会论文摘要集,2009:157Zhang G M, Liu X H, Fang F X, Zhang R H, Song H Z, Fang W K, You J H, Liu H B. Development of intergeneric complex between Erianthus arundinaceus and Saccharum spontaneum(GXAS07-6-1)and analysis of chromosome counting and morphological manifestation for the F1 hybrids from sugarcane(Saccharum spp.)with the intergeneric complex. The 2009Annual Academic Conference of Chinese Crop Society, 2009:157
[10] Gao Y J, Liu X H, Zhang R H, Zhou H, Liao J X, Duan W X, Zhang G M-Verification of progeny from crosses between sugarcane(Saccharum spp.)and an intergeneric hybrid(Erianthus arundinaceus x Saccharum spontaneum)with molecular makers. Sugar Tech, 2015, 17(1):31-35
[11]黄玉新,罗霆,林秀琴,张保青,周珊,杨翠芳,雷敬超,高轶静,段维兴,张革民,李杨瑞·斑茅割手密复合体(GXAS07-6-1)及其与甘蔗F1的GISH分析·植物遗传资源学报,2017,18(3):461-466Huang Y X,Luo T,Liu X H,Zhang B Q,Zhou S,Yang C F, Lei J C, Gao Y J, Duan W X, Zhang G M, Li Y RGISH analysis of intergeneric complex between Erianthus arundinaceus and Saccharum spontaneum(GXAS07-6-1)and its F1 hybrids from crosses with sugarcane(Saccharum spp.)·Journal of Plant Genetic Resources, 2017, 18(3):461-466
[12]钱路,安榆林,徐梅,杨晓军,朱宏斌·舞毒蛾不同地理种群基于AFLP分子标记的遗传分析.林业科学,2011,47(10):104-110Qian L, An Y L, Xu M, Yang X J, Zhu H B. AFLP analysis of different geographic populations of the gypsy moth, Lymantria dispar(Lepidoptera:Lymantriidae)·Scientia Silvae Sinicae,2011,47(10):104-110
[13]Selvi A,Nair N V,Noyer J L,Singh N K,Balasundaram N,Bansal K C, Koundal K R, Mohapatra T. AFLP analysis of the phenetic organization and genetic diversity in the sugarcane complex, Saccharum and Erianthus. Genetic Resources and Crop Evolution, 2006, 53:831-842
[14] Zhao S S, Chen X, Wan Q H. Assessment of genetic diversity in the forest musk deer(Moschus berezovskii)using microsatellite and AFLP markers. Chinese Science Bulletin, 2011,56(24):2565-2569
[15] Kaur S,Cogan N O,Ye G,Baillie R C,Hand M L,Ling A E,McGearey A K,Kaur J,Hopkins C J,Todorovic M,Mountford H,Edwards D,Batley J,Burton W,Salisbury P, Gororo N,Marcroft S, Kearney G, Smith K F, Forster J W, Spangenberg G C-Genetic map construction and QTL mapping of resistance to blackleg(Leptospha-eriamaculans)disease in Australian canola(Brassicanapus L.)cultivars, Theoretical and Applied Genetics,2009, 120(1):71-83
[16]孙子淇,张新友,徐静,张忠信,刘华,严玫,董文召,黄冰艳,韩锁义,汤丰收,刘志勇·河南省审定花生品种的指纹图谱构建·作物学报,2016, 42(10):1448-1461Sun Z Q, Zhang X Y, Xu J, Zhang Z X, Liu H, Yan M,Dong W Z, Huang B Y, Han S Y, Tang F S, Liu Z Y. DNA fingerprinting of peanut(Arachis hypogaea L.)varieties released in Henan province. Acta Agronomica Sinica, 2016,42(10):1448-1461
[17]吕品,于海峰,于志贤,张永虎,张艳芳,王婷婷,侯建华·向日葵高密度遗传连锁图谱构建及两种水分条件下芽期性状的QTL分析·作物学报,2017,43(1):19-30Lv P, Yu H F, Yu Z X, Zhang Y H, Zhang Y F, Wang T T,Hou J H. Construction of high-density genetic map and QTL mapping for seed germination traits in sunflower under two water conditions. Acta Agronomica Sinica, 2017,43(1):19-30
[18] Aitken K, Jackson P M, clntyre C L. A combination of AFLP and SSR markers provides extensive map coverage and identification of homo(eo)logous linkage groups in a sugarcane cultivar. Theoretical and Applied Genetics, 2005,100:789-801
[19]段会军,张彩英,李喜焕,郭小敏,王省芬,马峙英·河北省西瓜枯萎病菌生理小种鉴定与AFLP分析·中国农业科学,2007,40(5):925-931Duan H J, Zhang C Y, Li X H, Guo X M, Wang X F, Ma Z Y. Identification of physiological races and AFLP analysis of Fusarium oxyporum f sp niveum from Hebei province. Scientia Agricultura Sinica, 2007,40(5):925-931
[20] Lionneton E,Ravera S,Sanchez L,Aubert G,Delourme R,Ochatt S-Development of an AFLP-based link-age map and localization of QTLs for seed fatty acid content in condiment mustard(Brassicajuncea).Genome, 2002,45(6):1203-1215
[21] Salland C, Lorieux M, Roumen E, Tharreau D, Berruyer R,Svestasrani P, Grasmeur O, Ghesquire A, Notteghem J L.Identification of five new blast resistance genes in the highly blast resistant variety IR 64 using a QTL mapping strategy.Theoretical and Applied Genetics, 2003, 106(5):794-803
[22] Caballero A, Pereira M J G, Quesada H. Genomic distribution of AFLP markers relative to gene locations for different eukaryotic species. BMC Genomics, 2013, 14:528
[23]刘昔辉,张荣华,张革民,周会,宋焕忠,方位宽,区惠平,杨丽涛,李杨瑞·基于AFLP标记的河八王种质资源遗传多样性分析及分子身份证构建·华中农业大学学报,2016, 35(4):7-13Liu X H, Zhang R H, Zhang G M, Zhou H, Song H Z, Fang W K,Qu H P,Yang L T,Li Y R. Genetic diversity and molecular identification card construction of Narenga porphyrocoma(Hance)Bor germplasm based on ALFP markers. Journal of Huazhong Agricultural University, 2016, 35(4):7-13
[24]毛钧,刘新龙,苏火生,陆鑫,林秀琴,蔡青,范源洪·基于表型与分子数据的斑茅核心种质构建·植物遗传资源学报,2016,17(4):607-615Mao J,Liu X L,SuHS,Lu X,Lin X Q,Cai Q,Fan Y H.Constructing core collection of Saccharum arundinaceum L.based on phenotype and molecular markers. Journal of Plant Genetic Resources, 2016, 17(4):607-615
[25] Tagane S,Ponragdee W,Sansayawichai T,Sugimoto A,Terajima Y.Characterization and taxonomical note about Thai Erianthus germplasm collection:the morphology, flowering phenology and biogeography among E. procerus and three types of E. arundinaceus. Genetic Resources and Crop Evolution,2012, 59:769-781
[26]刘新龙,蔡青,马丽,应雄美,毛钧.甘蔗基因定位及分子标记辅助育种研究·西南农业学报,2006, 19(S):521-525Liu X L, Cai Q,Ma L,Ying X M,Mao J. Research advancements of locating gene and marker-assisted selection breeding of sugarcane. Southwest China Journal of Agricultural Sciences, 2006, 19(S):521-525
[27]黄东亮,覃肖良,廖青,高轶静,方锋学·高质量甘蔗基因组DNA的简便快速提取方法研究·生物技术通报,2010(5):101-106Huang D L,Qin X L,Liao Q,Gao Y J,Fang F X. Simple and rapid procedure for isolation of high quality genomic DNA from sugarcane. Biotechnol Bull, 2010(5):101-106
[28] Vos P, Hogers R, Bleeker M, Reijans M, vande Lee T, Homes M,Frijters A,Pot J,Peleman J,Kuiper M,Zabeau M. AFLP:a new technique for DNA fingerprinting. Nucleic Acids Research,1995,23(21):4407-4414
[29]刘昔辉,方锋学,高轶静,张荣华,宋焕忠,杨荣仲,方位宽,段维兴,罗霆,张革民,李杨瑞·斑茅割手密杂种后代真实性鉴定及遗传分析·作物学报,2012,38(5):914-920Liu X H, Fang F X, Gao Y J, Zhang R H, Song H Z, Yang R Z, Fang W K, Duan W X, Luo T, Zhang G M, Li Y RIdentification and genetic analysis of hybrid from cross between Erianthus arundinacius(Retz.)Jesws. and Saccharum spontaneum L. Acta Agronomica Sinica, 2012,38(5):914-920
[30]刘许辉.甘蔗与斑茅割手密复合体杂交后代的遗传分析·南宁:广西大学,2013Liu X H. Genetic analysis for the progenies from the cross Saccharum with the hybrid of Erianthus Arundinaceus(Retz)Jeswiet and Saccharum Spontaneum L. Nanning:Guangxi University, 2013
[31]庄南生,郑成木,黄东益,唐燕琼,高和琼·甘蔗种质遗传基础的AFLP分析·作物学报,2005,31(4):444-450Zhuang N S, Zheng C M, Huang D Y, Tang Y Q, Gao H Q.AFLP analysis for sugarcane germplasms. Acta Agronomica Sinica,2005,31(4):444-450
[32]刘家勇,赵培方,刘新龙,赵俊,杨昆,吴才文,应雄美,昝逢刚,陈学宽.68份国外甘蔗种质资源遗传多样性的AFLP分析·湖南农业大学学报:自然科学版,2013,39(5):466-470Liu J Y, Zhao P F, Liu X L, Zhao J, Yang K, Wu C W, Ying X M, Zan F G, Chen X K. Genetic diversity analysis on 68foreign sugarcane germplasms(Saccharum spp.)with AFLP technique. Journal of Hunan Agricultural University:Natural Sciences, 2013,39(5):466-470
[2]黄永吉,吴嘉云,刘少谋,邓祖湖,符成,林彦铨,李奇伟,黄忠兴,陈如凯·基于GISH的甘蔗与斑茅F1染色体遗传与核型分析·植物遗传资源学报,2014, 15(2):394-398Huang Y J, Wu J Y, Liu S M, Deng Z H, Fu C, Lin Y Q, Li Q W, Huang Z X, Chen R K. Chromosome genetic and karyotype analysis of F1 hybrids between Saccharum officinarum and Erianthus arundinaceus based on GISH-Journal of Plant Genetic Resources, 2014, 15(2):394-398
[3]高轶静,方锋学,刘昔辉,张荣华,宋焕忠,杨荣仲,罗霆,段维兴,游建华,张革民·甘蔗与斑茅割手密复合体杂交后代的分子标记鉴定·植物遗传资源学报,2012, 13(5):912-916Gao Y J, Fang F X, Liu X H, Zhang R H, Song H Z, Yang R Z, Luo T, Duan W X, You J H, Zhang G M. Identification of progeny from crosses between sugarcane(Saccharum spp.)and intergeneric hybrid complex(Erianthus arundinaceus x Saccharum spontaneum)with molecular markers. Journal of Plant GeneticResources,2012, 13(5):912-916
[4]中华人民共和国农业部·中国作物遗传资源·北京:中国农业出版社,1998:499-518Ministry of agriculture of the People's Republic of China.Crop genetic resources in China. Beijing:China Agricultural Publishs,1998:499-518
[5]陈辉,范源洪,史宪伟,蔡青,张明,张亚平.甘蔗细茎野生种(Saccharunsponta-neum L.)的遗传多样性和系统演化研究·作物学报,2001,27(5):645-652Chen H, Fan Y H, Shi X W, Cai Q, Zhang M, Zhang Y P. Research on genetic diversity and systemic evolution in Saccharum spontaneum L. Acta Agronomica Sinica, 2001,27(5):645-652
[6]陈如凯·现代甘蔗育种的理论与实践·北京:中国农业出版社,2003:11-12Chen R K.Theory and practice of modern sugarcane breeding.Beijing:China Agricultural Publishs, 2003:11-12
[7]王先宏,郭超,娄红波,李富生,何丽莲,何顺长,唐荣平,杨清辉·不同基因型割手密无性系的核型分析·植物遗传资源学报,2014, 15(4):912-917Wang X H,Guo C,Lou H B,Li F S,He L L,He S C.Tang R P, Yang Q H-Karyotype analysis of different Saccharum spontaneum genotype clones. Journal of Plant Genetic Resources, 2014, 15(4):912-917
[8]Lekshmi M, Pazhany A S, Sobhakumari V P, Premachandran N M-Nuclear and cytoplasmic contributions from Erianthus arundinaceus(Retz.)Jeswiet in a sugarcane hybrid clone confirmed through genomic in situ hybridization and cytoplasmic DNA polymorphism. Genetic Resources and Crop Evolution, 2017, 64:1553-1560
[9]张革民,刘昔辉,方锋学,张荣华,宋焕忠,方位宽,游建华,刘海彬·斑茅割手密复合体创制及其与甘蔗杂交F1的染色体计数和形态表现·2009年中国作物学会学术年会论文摘要集,2009:157Zhang G M, Liu X H, Fang F X, Zhang R H, Song H Z, Fang W K, You J H, Liu H B. Development of intergeneric complex between Erianthus arundinaceus and Saccharum spontaneum(GXAS07-6-1)and analysis of chromosome counting and morphological manifestation for the F1 hybrids from sugarcane(Saccharum spp.)with the intergeneric complex. The 2009Annual Academic Conference of Chinese Crop Society, 2009:157
[10] Gao Y J, Liu X H, Zhang R H, Zhou H, Liao J X, Duan W X, Zhang G M-Verification of progeny from crosses between sugarcane(Saccharum spp.)and an intergeneric hybrid(Erianthus arundinaceus x Saccharum spontaneum)with molecular makers. Sugar Tech, 2015, 17(1):31-35
[11]黄玉新,罗霆,林秀琴,张保青,周珊,杨翠芳,雷敬超,高轶静,段维兴,张革民,李杨瑞·斑茅割手密复合体(GXAS07-6-1)及其与甘蔗F1的GISH分析·植物遗传资源学报,2017,18(3):461-466Huang Y X,Luo T,Liu X H,Zhang B Q,Zhou S,Yang C F, Lei J C, Gao Y J, Duan W X, Zhang G M, Li Y RGISH analysis of intergeneric complex between Erianthus arundinaceus and Saccharum spontaneum(GXAS07-6-1)and its F1 hybrids from crosses with sugarcane(Saccharum spp.)·Journal of Plant Genetic Resources, 2017, 18(3):461-466
[12]钱路,安榆林,徐梅,杨晓军,朱宏斌·舞毒蛾不同地理种群基于AFLP分子标记的遗传分析.林业科学,2011,47(10):104-110Qian L, An Y L, Xu M, Yang X J, Zhu H B. AFLP analysis of different geographic populations of the gypsy moth, Lymantria dispar(Lepidoptera:Lymantriidae)·Scientia Silvae Sinicae,2011,47(10):104-110
[13]Selvi A,Nair N V,Noyer J L,Singh N K,Balasundaram N,Bansal K C, Koundal K R, Mohapatra T. AFLP analysis of the phenetic organization and genetic diversity in the sugarcane complex, Saccharum and Erianthus. Genetic Resources and Crop Evolution, 2006, 53:831-842
[14] Zhao S S, Chen X, Wan Q H. Assessment of genetic diversity in the forest musk deer(Moschus berezovskii)using microsatellite and AFLP markers. Chinese Science Bulletin, 2011,56(24):2565-2569
[15] Kaur S,Cogan N O,Ye G,Baillie R C,Hand M L,Ling A E,McGearey A K,Kaur J,Hopkins C J,Todorovic M,Mountford H,Edwards D,Batley J,Burton W,Salisbury P, Gororo N,Marcroft S, Kearney G, Smith K F, Forster J W, Spangenberg G C-Genetic map construction and QTL mapping of resistance to blackleg(Leptospha-eriamaculans)disease in Australian canola(Brassicanapus L.)cultivars, Theoretical and Applied Genetics,2009, 120(1):71-83
[16]孙子淇,张新友,徐静,张忠信,刘华,严玫,董文召,黄冰艳,韩锁义,汤丰收,刘志勇·河南省审定花生品种的指纹图谱构建·作物学报,2016, 42(10):1448-1461Sun Z Q, Zhang X Y, Xu J, Zhang Z X, Liu H, Yan M,Dong W Z, Huang B Y, Han S Y, Tang F S, Liu Z Y. DNA fingerprinting of peanut(Arachis hypogaea L.)varieties released in Henan province. Acta Agronomica Sinica, 2016,42(10):1448-1461
[17]吕品,于海峰,于志贤,张永虎,张艳芳,王婷婷,侯建华·向日葵高密度遗传连锁图谱构建及两种水分条件下芽期性状的QTL分析·作物学报,2017,43(1):19-30Lv P, Yu H F, Yu Z X, Zhang Y H, Zhang Y F, Wang T T,Hou J H. Construction of high-density genetic map and QTL mapping for seed germination traits in sunflower under two water conditions. Acta Agronomica Sinica, 2017,43(1):19-30
[18] Aitken K, Jackson P M, clntyre C L. A combination of AFLP and SSR markers provides extensive map coverage and identification of homo(eo)logous linkage groups in a sugarcane cultivar. Theoretical and Applied Genetics, 2005,100:789-801
[19]段会军,张彩英,李喜焕,郭小敏,王省芬,马峙英·河北省西瓜枯萎病菌生理小种鉴定与AFLP分析·中国农业科学,2007,40(5):925-931Duan H J, Zhang C Y, Li X H, Guo X M, Wang X F, Ma Z Y. Identification of physiological races and AFLP analysis of Fusarium oxyporum f sp niveum from Hebei province. Scientia Agricultura Sinica, 2007,40(5):925-931
[20] Lionneton E,Ravera S,Sanchez L,Aubert G,Delourme R,Ochatt S-Development of an AFLP-based link-age map and localization of QTLs for seed fatty acid content in condiment mustard(Brassicajuncea).Genome, 2002,45(6):1203-1215
[21] Salland C, Lorieux M, Roumen E, Tharreau D, Berruyer R,Svestasrani P, Grasmeur O, Ghesquire A, Notteghem J L.Identification of five new blast resistance genes in the highly blast resistant variety IR 64 using a QTL mapping strategy.Theoretical and Applied Genetics, 2003, 106(5):794-803
[22] Caballero A, Pereira M J G, Quesada H. Genomic distribution of AFLP markers relative to gene locations for different eukaryotic species. BMC Genomics, 2013, 14:528
[23]刘昔辉,张荣华,张革民,周会,宋焕忠,方位宽,区惠平,杨丽涛,李杨瑞·基于AFLP标记的河八王种质资源遗传多样性分析及分子身份证构建·华中农业大学学报,2016, 35(4):7-13Liu X H, Zhang R H, Zhang G M, Zhou H, Song H Z, Fang W K,Qu H P,Yang L T,Li Y R. Genetic diversity and molecular identification card construction of Narenga porphyrocoma(Hance)Bor germplasm based on ALFP markers. Journal of Huazhong Agricultural University, 2016, 35(4):7-13
[24]毛钧,刘新龙,苏火生,陆鑫,林秀琴,蔡青,范源洪·基于表型与分子数据的斑茅核心种质构建·植物遗传资源学报,2016,17(4):607-615Mao J,Liu X L,SuHS,Lu X,Lin X Q,Cai Q,Fan Y H.Constructing core collection of Saccharum arundinaceum L.based on phenotype and molecular markers. Journal of Plant Genetic Resources, 2016, 17(4):607-615
[25] Tagane S,Ponragdee W,Sansayawichai T,Sugimoto A,Terajima Y.Characterization and taxonomical note about Thai Erianthus germplasm collection:the morphology, flowering phenology and biogeography among E. procerus and three types of E. arundinaceus. Genetic Resources and Crop Evolution,2012, 59:769-781
[26]刘新龙,蔡青,马丽,应雄美,毛钧.甘蔗基因定位及分子标记辅助育种研究·西南农业学报,2006, 19(S):521-525Liu X L, Cai Q,Ma L,Ying X M,Mao J. Research advancements of locating gene and marker-assisted selection breeding of sugarcane. Southwest China Journal of Agricultural Sciences, 2006, 19(S):521-525
[27]黄东亮,覃肖良,廖青,高轶静,方锋学·高质量甘蔗基因组DNA的简便快速提取方法研究·生物技术通报,2010(5):101-106Huang D L,Qin X L,Liao Q,Gao Y J,Fang F X. Simple and rapid procedure for isolation of high quality genomic DNA from sugarcane. Biotechnol Bull, 2010(5):101-106
[28] Vos P, Hogers R, Bleeker M, Reijans M, vande Lee T, Homes M,Frijters A,Pot J,Peleman J,Kuiper M,Zabeau M. AFLP:a new technique for DNA fingerprinting. Nucleic Acids Research,1995,23(21):4407-4414
[29]刘昔辉,方锋学,高轶静,张荣华,宋焕忠,杨荣仲,方位宽,段维兴,罗霆,张革民,李杨瑞·斑茅割手密杂种后代真实性鉴定及遗传分析·作物学报,2012,38(5):914-920Liu X H, Fang F X, Gao Y J, Zhang R H, Song H Z, Yang R Z, Fang W K, Duan W X, Luo T, Zhang G M, Li Y RIdentification and genetic analysis of hybrid from cross between Erianthus arundinacius(Retz.)Jesws. and Saccharum spontaneum L. Acta Agronomica Sinica, 2012,38(5):914-920
[30]刘许辉.甘蔗与斑茅割手密复合体杂交后代的遗传分析·南宁:广西大学,2013Liu X H. Genetic analysis for the progenies from the cross Saccharum with the hybrid of Erianthus Arundinaceus(Retz)Jeswiet and Saccharum Spontaneum L. Nanning:Guangxi University, 2013
[31]庄南生,郑成木,黄东益,唐燕琼,高和琼·甘蔗种质遗传基础的AFLP分析·作物学报,2005,31(4):444-450Zhuang N S, Zheng C M, Huang D Y, Tang Y Q, Gao H Q.AFLP analysis for sugarcane germplasms. Acta Agronomica Sinica,2005,31(4):444-450
[32]刘家勇,赵培方,刘新龙,赵俊,杨昆,吴才文,应雄美,昝逢刚,陈学宽.68份国外甘蔗种质资源遗传多样性的AFLP分析·湖南农业大学学报:自然科学版,2013,39(5):466-470Liu J Y, Zhao P F, Liu X L, Zhao J, Yang K, Wu C W, Ying X M, Zan F G, Chen X K. Genetic diversity analysis on 68foreign sugarcane germplasms(Saccharum spp.)with AFLP technique. Journal of Hunan Agricultural University:Natural Sciences, 2013,39(5):466-470