食用油脂过氧化值流动注射分析仪研制
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摘要
建立流动注射食用油脂过氧化值测定方法。基于流动注射分析—分光光度比色法研制了一种食用油脂过氧化值流动注射分析仪,研制了电动变向三通道液流阀和微恒流泵;阀控制载液流向,三个溶液开关的九个溶液孔在阀体同一圆周上,占用空间小,阀体旋转三十度即可实现三种载液同时换向;泵控制样品溶液注入,旋桨旋转后在锥形圆台腔可形成高压溶液区,转动不接触无磨损,死体积小。方法的线性范围为5~170μmol/L,线性相关系数为0.9995,检出限为3.57μmol/L,回收率在93%~104%之间。本方法用于快速检测菜籽油、循环火锅油和回收油的过氧化值,对本方法和国标方法的测定结果进行t检验分析,两种方法检测结果无显著性差异。
A flow injection peroxide value of edible oil measuring method was established。A kind of flow injection measuring peroxide value of edible oil instrument was developed based on the flow injection analysis-spectrophotometric colorimetry,the electric three-channel flow valve and the constant flow micro-pump was developed.The flow direction of the carrier liquid was controlled by the valve,nine solution holes of three switches were on the same circumference of the valve body,occupying less space,three kinds of carrier fluid were changed direction at the same time by thirty degrees rotated by the valve body. the injection of a sample solution was controlled by the pump,the high-pressure solution was formed in the conical cavity by the propeller rotating,and the rotating without contact and friction,and the dead volume was small. The linear range for the concentration of peroxide value was from 5 to 170 μmol / L with a correlation coefficient of 0.9995,the limit of detection was3.57 μmol / L,the recovery rate was between 93% ~104%.This method is used for rapid detection of peroxide value of rapeseed oil,recycled oil and hot pot oil,the determination results of this method and national standard method for T-test analysis,testing results of two methods have no significant difference.
A flow injection peroxide value of edible oil measuring method was established。A kind of flow injection measuring peroxide value of edible oil instrument was developed based on the flow injection analysis-spectrophotometric colorimetry,the electric three-channel flow valve and the constant flow micro-pump was developed.The flow direction of the carrier liquid was controlled by the valve,nine solution holes of three switches were on the same circumference of the valve body,occupying less space,three kinds of carrier fluid were changed direction at the same time by thirty degrees rotated by the valve body. the injection of a sample solution was controlled by the pump,the high-pressure solution was formed in the conical cavity by the propeller rotating,and the rotating without contact and friction,and the dead volume was small. The linear range for the concentration of peroxide value was from 5 to 170 μmol / L with a correlation coefficient of 0.9995,the limit of detection was3.57 μmol / L,the recovery rate was between 93% ~104%.This method is used for rapid detection of peroxide value of rapeseed oil,recycled oil and hot pot oil,the determination results of this method and national standard method for T-test analysis,testing results of two methods have no significant difference.
引文
[1]同秀娥.可见分光光度法测定食用油脂过氧化值[J].科协论坛,2007,7(下):12~13.
[2]邓鹏,程永强,薛文通.油脂氧化及其氧化稳定性测定方法[J].食品科学,2005,26:196~199.
[3]李书国,薛文通,张惠.食用油脂过氧化值分析检测方法研究进展[J].粮食与油脂,2007,(7):35~38.
[4]李昌模.薄层色谱法测定油脂中极性化合物含量[J].中国油脂,2007,32(11)77~79.
[5]GB/T 5538-2005/ISO 3960:2001,动植物油脂过氧化值测定[S].
[6]GB/T 5009.37-2003,食用植物油卫生标准的分析方法[S].
[7]Kang Tian,Purnendu K.Dasgupta.Automated Measurement of Lipid Hydroperoxides in Oil and Fat Samples by Flow Injection Photometry[J].Anal.Chem.,1999,71,2053~2058.
[8]汤乐弘,葛玉松.硫氰酸铵显色分光光度法测定晕可平糖浆中铁的含量[J].时珍国医国药,2001,12(10):891~892.
[9]郑用熙.分析化学中的数理统计方法[M].北京:科学出版社,1986.220~225.
[10]马斌荣.医学统计学[M].北京:人民卫生出版社,2005.246.
[2]邓鹏,程永强,薛文通.油脂氧化及其氧化稳定性测定方法[J].食品科学,2005,26:196~199.
[3]李书国,薛文通,张惠.食用油脂过氧化值分析检测方法研究进展[J].粮食与油脂,2007,(7):35~38.
[4]李昌模.薄层色谱法测定油脂中极性化合物含量[J].中国油脂,2007,32(11)77~79.
[5]GB/T 5538-2005/ISO 3960:2001,动植物油脂过氧化值测定[S].
[6]GB/T 5009.37-2003,食用植物油卫生标准的分析方法[S].
[7]Kang Tian,Purnendu K.Dasgupta.Automated Measurement of Lipid Hydroperoxides in Oil and Fat Samples by Flow Injection Photometry[J].Anal.Chem.,1999,71,2053~2058.
[8]汤乐弘,葛玉松.硫氰酸铵显色分光光度法测定晕可平糖浆中铁的含量[J].时珍国医国药,2001,12(10):891~892.
[9]郑用熙.分析化学中的数理统计方法[M].北京:科学出版社,1986.220~225.
[10]马斌荣.医学统计学[M].北京:人民卫生出版社,2005.246.