HPLC同步测定泽兰配方颗粒指纹图谱与咖啡酸、迷迭香酸含量
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摘要
目的:建立泽兰配方颗粒的HPLC指纹图谱测定方法,并同时对咖啡酸、迷迭香酸的含量进行测定,为泽兰配方颗粒的质量评价提供依据。方法:采用HPLC法,色谱柱为C18,以乙腈-0.1%磷酸为流动相进行梯度洗脱,检测波长为325 nm,流速为1.0 mL/min,测定了10批泽兰配方颗粒。结果:指纹图谱检测结果中共呈现7个特征峰。含量测定结果表明,咖啡酸、迷迭香酸分别在0.001～0.205 4μg、0.005 2～1.030 4μg范围内的线性关系良好,r=1,两种成分平均回收率分别为99.2%、104.23%,RSD分别为1.23%、1.45%。结论:建立了同步测定泽兰配方颗粒指纹图谱与咖啡酸、迷迭香酸含量的方法,方法操作简便,准确,重现性好,可用于泽兰配方颗粒的质量研究和评价。
Objective: To establisha the fingerprint of Zelan Granules by HPLC, and to determine the contents of caffeic acid and rosmarinic acid, thus to provide evidence for the quality evaluation of Zelan Granules. Methods: The chromatography column of C18 was applied, the mobile phase consisted of acetonitrile and 0.1% phosphoric acid with the gradient mode at a flow rate of 1 mL/min, the wavelength was set at 325 nm. Ten batches of Zelan Granules were measured. Results: The fingerprint showed seven characteristic peaks. The content determination showed that the linear relationship of caffeic acid and rosmarinic acid were good(r=1), which were at 0.001 0～0.205 4 μg and 0.005 2～1.030 4 μg respectively, the average recovery rates were 99.20% and 104.23%, and RSD were 1.23% and 1.45%. Conclusion: A method for simultaneous determination of the fingerprint and the contents of caffeic acid and rosmarinic acid in Zelan Granules were established. The method is accurate, stable and credible. It can be used for the quality study and evaluation of Zelan Granules.
Objective: To establisha the fingerprint of Zelan Granules by HPLC, and to determine the contents of caffeic acid and rosmarinic acid, thus to provide evidence for the quality evaluation of Zelan Granules. Methods: The chromatography column of C18 was applied, the mobile phase consisted of acetonitrile and 0.1% phosphoric acid with the gradient mode at a flow rate of 1 mL/min, the wavelength was set at 325 nm. Ten batches of Zelan Granules were measured. Results: The fingerprint showed seven characteristic peaks. The content determination showed that the linear relationship of caffeic acid and rosmarinic acid were good(r=1), which were at 0.001 0～0.205 4 μg and 0.005 2～1.030 4 μg respectively, the average recovery rates were 99.20% and 104.23%, and RSD were 1.23% and 1.45%. Conclusion: A method for simultaneous determination of the fingerprint and the contents of caffeic acid and rosmarinic acid in Zelan Granules were established. The method is accurate, stable and credible. It can be used for the quality study and evaluation of Zelan Granules.
引文
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[10] 宋艺君,郭涛,孙婧,等.陕产延胡索产地加工与炮制一体化HPLC指纹图谱研究[J].中医药信息,2018,35(5):49-54.
[11] 范卓文,谢明,李晓梅,等.东北三省不同采收期五味子HPLC指纹图谱的研究[J].中医药信息,2015,32(5):57-60.
[2] 广东省食品药品监督管理局编.《广东省中药配方颗粒标准》(第一册)[M].北京:中国医药科技出版社,2013.
[3] 刘君.泽兰的化学成分及药理研究进展[J].辽宁中医药大学学报,2008,10(1):23-24.
[4] 任强,王红玲,周学刚,等.泽兰的化学成分、质量分析及药理作用研究进展[J].中国药房,2015,26(18):2588-2592.
[5] 李超.泽兰的化学成分及其质量评价研究[D].南京:南京中医药大学,2013:3-10.
[6] 张静,彭海燕.泽兰药理作用研究进展[J].河北中医,2015,37(3):460-462.
[7] 王昆,杨文婧,张丽华.分析泽兰化学成分、质量及药理作用[J].药物应用,2018(10):97-98.
[8] 陈慕媛,黄月纯,刘东辉,等.泽兰饮片及不同部位HPLC指纹图谱研究[J].中成药,2010,32(12):2029-2031.
[9] 童欣,贺凡珍,彭维,等.HPLC法同时测定泽兰中咖啡酸和迷迭香酸的含量[J].中药材,2012,35(2):246-247.
[10] 宋艺君,郭涛,孙婧,等.陕产延胡索产地加工与炮制一体化HPLC指纹图谱研究[J].中医药信息,2018,35(5):49-54.
[11] 范卓文,谢明,李晓梅,等.东北三省不同采收期五味子HPLC指纹图谱的研究[J].中医药信息,2015,32(5):57-60.