池塘养殖条件下恩诺沙星及其代谢产物在斑点叉尾鮰体内的残留消除规律
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摘要
在池塘养殖条件下,每天以20mg/kg和40mg/kg剂量的恩诺沙星药饵投喂斑点叉尾鮰,周期为5d,研究恩诺沙星及其代谢产物在斑点叉尾鮰体内的分布与残留消除规律。分析结果显示,低剂量组和高剂量组斑点叉尾鮰肝脏中恩诺沙星的达峰时间分别在给药期间第3天和第4天,达峰时的药物含量分别为12.24、15.97mg/kg。低剂量组肝脏、肌肉和血液中的消除曲线方程分别为C=0.25e-0.136 t、C=0.31e-0.082 t和C=0.23e-1.37 t,消除半衰期分别为5.10、8.43和5.06d。高剂量组肝脏、肌肉和血液中的消除曲线方程分别为C=0.70e-0.173 t、C=0.50e-0.096 t和C=1.08e-0.182 t,消除半衰期分别为4.00、7.22和3.81d。恩诺沙星在斑点叉尾鮰体内可代谢为环丙沙星。在用药138d时,低剂量组鱼体肌肉中恩诺沙星含量为0.013mg/kg;高剂量组为0.019mg/kg。药物在斑点叉尾鮰各组织中的消除顺序依次为血液>肝脏>肌肉。
The depletion rules of enrofloxacin and its metabolite residues were studied in channel catfish(Ietalurus punetaus)feeding with 20 mg/kg and 40 mg/kg enrofloxacin for 5 dunder pond conditions.The results showed that the peaking time(tmax)of enrofloxacin in the liver was 3 dat low dose,and 4 dat high dose during the period of treatment,and the corresponding maximal drug concentration(Cmax)was 12.24 mg/kg and 15.97 mg/kg,respectively.The equations of elimination curve of enrofloxacin in the liver,muscle and blood were C=0.25 e-0.136 t,C=0.31 e-0.082 t and C=0.23 e-1.37 t,and the elimination half-lives were 5.10 d,8.43 dand 5.06 dat dose of 30 mg/kg,respectively.The equations of elimination curve of the drug were C=0.70 e-0.173 t,C=0.50 e-0.096 t and C=1.08 e-0.182 t,and the elimination half-lives were 4.00 d,7.22 dand 3.81 dat dose of 60 mg/kg,respectively.Enrofloxacin was changed into ciprofloxacin in channel catfish.The concentration of enrofloxacin in the muscle was 0.013 mg/kg after 138 dat low dose,and 0.019 mg/kg after 138 dat high dose.The elimination rate of the drug in various tissues was in the order of blood,liver and muscle.
The depletion rules of enrofloxacin and its metabolite residues were studied in channel catfish(Ietalurus punetaus)feeding with 20 mg/kg and 40 mg/kg enrofloxacin for 5 dunder pond conditions.The results showed that the peaking time(tmax)of enrofloxacin in the liver was 3 dat low dose,and 4 dat high dose during the period of treatment,and the corresponding maximal drug concentration(Cmax)was 12.24 mg/kg and 15.97 mg/kg,respectively.The equations of elimination curve of enrofloxacin in the liver,muscle and blood were C=0.25 e-0.136 t,C=0.31 e-0.082 t and C=0.23 e-1.37 t,and the elimination half-lives were 5.10 d,8.43 dand 5.06 dat dose of 30 mg/kg,respectively.The equations of elimination curve of the drug were C=0.70 e-0.173 t,C=0.50 e-0.096 t and C=1.08 e-0.182 t,and the elimination half-lives were 4.00 d,7.22 dand 3.81 dat dose of 60 mg/kg,respectively.Enrofloxacin was changed into ciprofloxacin in channel catfish.The concentration of enrofloxacin in the muscle was 0.013 mg/kg after 138 dat low dose,and 0.019 mg/kg after 138 dat high dose.The elimination rate of the drug in various tissues was in the order of blood,liver and muscle.
引文
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[2]ZHANG D F,LI A H,XIE J,et al.In vitro antibacterial effect of berberine hydrochloride and enrofloxacin to fish pathogenic bacteria[J].Aquaculture research,2010,41:1095-1100.
[3]叶建美,吴康,吴洪丽,等.恩诺沙星抗菌效果及药代动力学研究进展[J].湖北农业科学,2015,54(23):5813-5815.
[4]方晓明,丁卓平.动物源性食品兽药残留分析[M].北京:化学工业出版社,2008:210.
[5]宋洪波,吴光红,沈美芳,等.恩诺沙星在水产品中残留的风险评估[J].渔业现代化,2008,35(5):39-42.
[6]徐维海,林黎明,朱校斌,等.恩诺沙星及其代谢产物在吉富罗非鱼、中国对虾体内的残留规律研究[J].水产科学,2004,23(7):5-8.
[7]杨秋红,艾晓辉,刘永涛,等.不同温度下恩诺沙星及其代谢物在斑点叉尾鮰各组织中的残留及消除规律比较[J].水生生物学报,2017,41(4):781-786.
[8]张德云,匡维华,郑映钦.恩诺沙星在日本鳗鲡体内残留消除规律研究[J].水产科学,2007,26(4):210-213.
[9]李娜,李健,王群.恩诺沙星在养殖大菱鲆体内的残留及消除规律[J].渔业科学进展,2009,30(2):26-33.
[10]刘艳辉,李改娟,祖岫杰,等.水温和药物剂量对德国锦鲤体内氟苯尼考和氟苯尼考胺残留的影响[J].大连海洋大学学报,2013,28(2):127-132.
[11]赵冬梅,李燕,卢叶竑.药物代谢研究在新药开发中的作用[J].药学学报,2000,35(2):156-160.
[12]唐锡招,李兆新,邢丽红,等.恩诺沙星代谢产物研究进展[J].中国渔业质量与标准,2015,5(2):65-74.
[13]张雅斌,刘艳辉,张祚新,等.恩诺沙星在鲤鱼体内的药效学及药代动力学[J].大连水产学院学报,2004,19(4):239-242.
[14]WALKER R D.The use of fluoroquinolones for companion animal antimicrobial therapy[J].Aust Vet J,2000,78(2):84-90.
[15]林维宣,王凤池.各国食品中农药兽药残留限量规定[M].大连:大连海事大学出版社,2002:1353.