十六个德国鸢尾品种的耐热性评价
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摘要
以16个德国鸢尾品种叶片为试材,在高温条件下测定叶片中叶绿素、丙二醛(MDA)、脯氨酸和可溶性蛋白质含量及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性,利用主成分、隶属函数及聚类分析方法对测定指标进行评价排序,综合鉴定比较16个德国鸢尾品种的耐热特性,以期为研究和利用德国鸢尾耐热品种提供参考依据。结果表明:不同品种在常温下的各项生理指标以及高温胁迫后的变化幅度各不相同;高温胁迫下,德国鸢尾各个品种的叶片中叶绿素含量均有不同程度降低;SOD与POD活性增强;MDA、脯氨酸及可溶性蛋白质含量升高;结合主成分分析、隶属函数值排序,16个德国鸢尾品种间耐热性强弱依次为"魂断蓝桥""PartyDress""Galamadrid""音箱""黑骑士""Ninbeiunge""Queen""Jessica""不朽白""法国回声""金娃娃""笛声""洋娃娃""黄莲""皇家十字军""短梦"。通过检测高温胁迫下德国鸢尾叶片生理指标,并利用主成分和隶属函数分析建立德国鸢尾耐热性的综合鉴定与评价体系,适用于耐热型德国鸢尾品种资源的评价与筛选。
The physiological index was detected and analyzed in order to provide references for evaluating and screening heat-tolerant cultivars of Iris germanica.Under high-temperature stress,the physiological indices including the contents of chlorophyll,malondialdehyde,soluble protein and proline,and activities of antioxidant was determined.Then the heat-tolerances of 16 cultivars were ranked based on principal components analysis and subordinate function.The results showed that different cultivars showed varied levels of physiological indices.The physiological detection showed that chlorophyll contents were reduced in each cultivar,and activities of SOD and POD were increased,while the contents of MDA,proline and soluble protein were higher after the high temperature treatment.Combined with PCA and subordinate function analysis,heat tolerance of 16 cultivars were ranked as'HDLQ''PartyDress' 'Galamadrid''YX''HQS''Ninbeiunge''Queen''Jessica''BXB''FGHS''JWW''DS''YWW''HL''Royal''DM'.The established evaluation method based on PCA and subordinate function was feasible for evaluating and selecting heat-tolerant cultivars of Iris germanica.
The physiological index was detected and analyzed in order to provide references for evaluating and screening heat-tolerant cultivars of Iris germanica.Under high-temperature stress,the physiological indices including the contents of chlorophyll,malondialdehyde,soluble protein and proline,and activities of antioxidant was determined.Then the heat-tolerances of 16 cultivars were ranked based on principal components analysis and subordinate function.The results showed that different cultivars showed varied levels of physiological indices.The physiological detection showed that chlorophyll contents were reduced in each cultivar,and activities of SOD and POD were increased,while the contents of MDA,proline and soluble protein were higher after the high temperature treatment.Combined with PCA and subordinate function analysis,heat tolerance of 16 cultivars were ranked as'HDLQ''PartyDress' 'Galamadrid''YX''HQS''Ninbeiunge''Queen''Jessica''BXB''FGHS''JWW''DS''YWW''HL''Royal''DM'.The established evaluation method based on PCA and subordinate function was feasible for evaluating and selecting heat-tolerant cultivars of Iris germanica.
引文
[1] PETER A,MOPPER S.Delayed and carryover effects of salinity on flowering in Iris hexagons(Iridaceae)[J].American Journal of Botany,2002,89(11):1847-1851.
[2] WANG Y Y,MOPPER S.HASENSTEIN K H.Effects of salinity on endogenous ABA,IAA,JA,and SA in Iris hexagona[J].Journal of Chemical Ecology,2001,27(2):327-342.
[3] MAO J,XU H L,GUO C X,et al.Involvement of Ca2+in regulation of physiological indices and heat shock factor expression in four Iris germanica cultivars under high temperature stress[J].Journal of American Society Horticultural Sci,2014,139:678-698.
[4]许凤,瞿素萍,杨秀梅,等.20个德国鸢尾品种的引种栽培研究[J].中国农学通报,2018,34(28):67-72.
[5]童俊,周媛,毛静,等.鸢尾属部分园艺品种遗传多样性的ISSR分析[J].北方园艺,2015(10):104-107.
[6]郭彩霞.鸢尾属植物遗传多样性评价及干旱高温胁迫生理响应机制[D].武汉:华中农业大学,2016.
[7]芦建国,仲玉婷.外源GA3和SA对德国鸢尾耐盐性的影响[J].江西农业学报,2011(4):49-52.
[8]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[9] BEYER W F,FRIDOVICH I.Assaying of superoxide dismutase activity:Some large consequences of minor changes in conditions[J].Anal Biochem,1987,161:559-566.
[10]OMRAN R G.Peroxide levels and the activities of catalase,peroxidase,and indoleacetic acid oxidase during and after chilling cucumber seedling[J].Plant Physiol,1980,65:407-408.
[11]BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72:248-254.
[12]谢志坚.农业科学中的模糊数学方法[M].武汉:华中理工大学出版社,1983.
[13]WAHID A,GELANI S,ASHRAF M,et al.Heat tolerance in plants:An overview[J].Environmental and Experimental Botany,2007,61(3):199-223.
[14]SCHWANZ P,POLLE A.Growth under elevated CO2ameliorates defenses against photo-oxidative stress in poplar(Populus alba)[J].Environmental and Experimental Botany,2001,45(1):43-53.
[15]MARTINDALE J L,HOLBROOK N J.Cellular response to oxidative stress:Signaling for suicide and survival[J].Journal of Cellular Physiol,2002,192:1-15.
[16]曲复宁,王云山,张敏,等.高温胁迫对仙客来根系活力和叶片生化指标的影响[J].华北农学报,2002,17(1):127-131.
[17]SHI Q H,BAO Z Y,ZHU Z J,et al.Effect of different treatments of salicylic acid on heat tolerance,chlorophyll fluorescence,and antioxidant enzyme activity in seedlings of Cucumis sativa L.[J].Plant Growth Regul,2006,48:127-135.
[18]毛静,周媛,童俊,等.不同耐热性有髯鸢尾品种在高温胁迫下的生理响应比较[M]//中国观赏园艺研究进展.北京:中国林业出版社,2015:467-476.
[19]申惠翡,赵冰.杜鹃花品种耐热性评价及其生理机制研究[J].植物生理学报,2018,54(2):335-345.
[20]杨伟.菊花花器官耐热性研究及外源NO对菊花耐热性的影响[D].南京:南京农业大学,2010.
[21]杨伟,陈发棣,陈素梅,等.不同菊花品种不同开花阶段舌状花耐热性比较[J].南京农业大学学报,2011,34(2):47-53.
[22]吴盼婷,王江民,沈佳逾,等.不同菊花品种根系、地上部和叶片相关指标分析及其抗逆性评价[J].植物资源与环境学报,2017,26(2):46-54.
[2] WANG Y Y,MOPPER S.HASENSTEIN K H.Effects of salinity on endogenous ABA,IAA,JA,and SA in Iris hexagona[J].Journal of Chemical Ecology,2001,27(2):327-342.
[3] MAO J,XU H L,GUO C X,et al.Involvement of Ca2+in regulation of physiological indices and heat shock factor expression in four Iris germanica cultivars under high temperature stress[J].Journal of American Society Horticultural Sci,2014,139:678-698.
[4]许凤,瞿素萍,杨秀梅,等.20个德国鸢尾品种的引种栽培研究[J].中国农学通报,2018,34(28):67-72.
[5]童俊,周媛,毛静,等.鸢尾属部分园艺品种遗传多样性的ISSR分析[J].北方园艺,2015(10):104-107.
[6]郭彩霞.鸢尾属植物遗传多样性评价及干旱高温胁迫生理响应机制[D].武汉:华中农业大学,2016.
[7]芦建国,仲玉婷.外源GA3和SA对德国鸢尾耐盐性的影响[J].江西农业学报,2011(4):49-52.
[8]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[9] BEYER W F,FRIDOVICH I.Assaying of superoxide dismutase activity:Some large consequences of minor changes in conditions[J].Anal Biochem,1987,161:559-566.
[10]OMRAN R G.Peroxide levels and the activities of catalase,peroxidase,and indoleacetic acid oxidase during and after chilling cucumber seedling[J].Plant Physiol,1980,65:407-408.
[11]BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72:248-254.
[12]谢志坚.农业科学中的模糊数学方法[M].武汉:华中理工大学出版社,1983.
[13]WAHID A,GELANI S,ASHRAF M,et al.Heat tolerance in plants:An overview[J].Environmental and Experimental Botany,2007,61(3):199-223.
[14]SCHWANZ P,POLLE A.Growth under elevated CO2ameliorates defenses against photo-oxidative stress in poplar(Populus alba)[J].Environmental and Experimental Botany,2001,45(1):43-53.
[15]MARTINDALE J L,HOLBROOK N J.Cellular response to oxidative stress:Signaling for suicide and survival[J].Journal of Cellular Physiol,2002,192:1-15.
[16]曲复宁,王云山,张敏,等.高温胁迫对仙客来根系活力和叶片生化指标的影响[J].华北农学报,2002,17(1):127-131.
[17]SHI Q H,BAO Z Y,ZHU Z J,et al.Effect of different treatments of salicylic acid on heat tolerance,chlorophyll fluorescence,and antioxidant enzyme activity in seedlings of Cucumis sativa L.[J].Plant Growth Regul,2006,48:127-135.
[18]毛静,周媛,童俊,等.不同耐热性有髯鸢尾品种在高温胁迫下的生理响应比较[M]//中国观赏园艺研究进展.北京:中国林业出版社,2015:467-476.
[19]申惠翡,赵冰.杜鹃花品种耐热性评价及其生理机制研究[J].植物生理学报,2018,54(2):335-345.
[20]杨伟.菊花花器官耐热性研究及外源NO对菊花耐热性的影响[D].南京:南京农业大学,2010.
[21]杨伟,陈发棣,陈素梅,等.不同菊花品种不同开花阶段舌状花耐热性比较[J].南京农业大学学报,2011,34(2):47-53.
[22]吴盼婷,王江民,沈佳逾,等.不同菊花品种根系、地上部和叶片相关指标分析及其抗逆性评价[J].植物资源与环境学报,2017,26(2):46-54.