新疆维吾尔族、汉族抗结核药物性肝损伤患者IL-10分泌水平的研究
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摘要
目的探讨汉族、维吾尔族抗结核药物诱导的肝损伤(ATDLI)患者IL-10分泌量,比较两民族之间存在的差异。方法选取新疆维吾尔自治区胸科医院、石河子大学医学院第一附属医院确诊的ATDLI患者。采用双抗体夹心ELISA测定患者IL-10的分泌量。比较两个民族以及两个民族不同性别、不同肝损伤程度的患者血清IL-10的表达量。结果共收集ATDLI汉族组患者100例,维吾尔族组患者135例。维吾尔族组ATDLI患者IL-10分泌量[(56. 30±17. 24) pg/mL]高于汉族组[(45. 81±11. 04) pg/mL],差异有统计学意义(P <0. 0001)。汉族轻度、中度及重度肝损伤组患者血清IL-10水平分别为(44. 73±9. 10)、(47. 39±10. 58)、(49. 52±13. 21) pg/mL;维吾尔族轻度、中度及重度肝损伤组患者血清IL-10水平分别为(52. 30±15. 24)、(56. 84±13. 71)、(58. 62±14. 38) pg/mL。组间比较显示,IL-10的分泌量汉族、维吾尔族中重度肝损伤组患者均高于轻度肝损伤组患者(均P <0. 05)。结论 ATDLI患者IL-10分泌量随肝损伤程度的加重而升高。ATDLI患者IL-10分泌量维吾尔族高于汉族。
Objective To evaluate the secretion level of interleukin-10( IL-10) in Han and Uyghur nationality patients with anti-tuberculosis drug-induced liver injury( ATDLI),and compare the difference between two nationalities.Methods Patients who were diagnosed with ATDLI in Chest Hospital of Xinjiang Uyghur Autonomous Region and the First Affiliated Hospital of Medical College of Shihezi University were selected. Secretion level of IL-10 in patients was detected by double antibody sandwich ELISA. Expression level of serum IL-10 in patients of two nationalities,different genders,and different extent of liver injury of two nationalities were compared. Results A total of 100 ATDLI Han and 135 Uyghur nationality patients were collected. The secretion level of IL-10 in Uyghur nationality ATDLI patients was higher than that in Han nationality patients( [56. 30 ± 17. 24]pg/mL VS [45. 81 ± 11. 04]pg/mL,P <0. 0001). Serum levels of IL-10 in patients with mild,moderate,and severe liver injury in Han nationality patients were( 44. 73 ± 9. 10) pg/mL,( 47. 39 ± 10. 58) pg/m L,and(49. 52 ± 13. 21)pg/m L,respectively; in Uyghur nationality patients were(52. 30 ± 15. 24) pg/m L,(56. 84 ± 13. 71) pg/m L,and(58. 62 ± 14. 38) pg/m L,respectively. Group comparison showed that the secretion level of serum IL-10 of patients with moderate and severe liver injury in Han and Uyghur nationalities were both higher than that in patients with mild liver injury( both P < 0. 05). Conclusion Secretion level of IL-10 in patients with ATDLI increases with the severity of liver injury. Secretion level of IL-10 in Uyghur nationality ATDLI patients is higher than that in Han nationality ATDLI patients.
Objective To evaluate the secretion level of interleukin-10( IL-10) in Han and Uyghur nationality patients with anti-tuberculosis drug-induced liver injury( ATDLI),and compare the difference between two nationalities.Methods Patients who were diagnosed with ATDLI in Chest Hospital of Xinjiang Uyghur Autonomous Region and the First Affiliated Hospital of Medical College of Shihezi University were selected. Secretion level of IL-10 in patients was detected by double antibody sandwich ELISA. Expression level of serum IL-10 in patients of two nationalities,different genders,and different extent of liver injury of two nationalities were compared. Results A total of 100 ATDLI Han and 135 Uyghur nationality patients were collected. The secretion level of IL-10 in Uyghur nationality ATDLI patients was higher than that in Han nationality patients( [56. 30 ± 17. 24]pg/mL VS [45. 81 ± 11. 04]pg/mL,P <0. 0001). Serum levels of IL-10 in patients with mild,moderate,and severe liver injury in Han nationality patients were( 44. 73 ± 9. 10) pg/mL,( 47. 39 ± 10. 58) pg/m L,and(49. 52 ± 13. 21)pg/m L,respectively; in Uyghur nationality patients were(52. 30 ± 15. 24) pg/m L,(56. 84 ± 13. 71) pg/m L,and(58. 62 ± 14. 38) pg/m L,respectively. Group comparison showed that the secretion level of serum IL-10 of patients with moderate and severe liver injury in Han and Uyghur nationalities were both higher than that in patients with mild liver injury( both P < 0. 05). Conclusion Secretion level of IL-10 in patients with ATDLI increases with the severity of liver injury. Secretion level of IL-10 in Uyghur nationality ATDLI patients is higher than that in Han nationality ATDLI patients.
引文
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[21]杜若甫.我国的人类群体遗传学研究[J].生物学通报,1997,32(7):9-12.
[22]艾琼华,肖辉,赵建新,等.维吾尔族的体质特征研究[J].人类学学报,1993,12(4):357-364.
[23]袁义达,杜若甫.中国十七个民族间的遗传距离的初步研究[J].遗传学报,1983,10(5):398-405.
[2] Sabat R,Grütz G,Warszawska K,et al. Biology of interleukin-10[J]. Cytokine Growth Factor Rev,2010,21(5):331-344.
[3]李晨,邢少军,段学章,等.乙型肝炎肝硬化患者外周血调节性T细胞频率及血清IL-1β、IL-6和IL-10水平的变化[J].实用肝脏病杂志,2012,15(3):244-246.
[4] Van Dyke AL,Cote ML,Wenzlaff AS,et al. Cytokine SNPs:Comparison of allele frequencies by race and implications for future studies[J]. Cytokine,2009,46(2):236-244.
[5]杨津明,杰恩斯·斯马胡勒,邰新蓉,等.新疆维吾尔自治区2010—2011年结核病流行病学抽样调查结果分析[J].中国防痨杂志,2013,35(12):960-964.
[6]中华医学会结核病学分会.肺结核诊断和指南[J].中华结核和呼吸杂志,2001,24(1):70-73.
[7]中华医学会结核病学分会.抗结核药所致药物性肝损伤诊断与处理专家建议[J].中华结核和呼吸杂志,2013,36(10):732-736.
[8]麻斌喜,陈伟岚,郑甜,等.汉族、维吾尔族抗结核药物性肝损害患者血清TNF-α表达水平的研究[J].中国医院药学杂志,2017,37(24):2456-2459.
[9]麻斌喜.新疆维吾尔族、汉族抗结核药物性肝损害患者血清TNF-α表达水平的研究[D].乌鲁木齐:新疆医科大学,2017.
[10] Hyodo N,Nakamura I,Imawari M. Hepatitis B core antigen stimulates interleukin-10 secretion by both T cells and monocytes from peripheral blood of patients with chronic hepatitis B virus infection[J]. Clin Exp Immunol,2004,135:462-466.
[11]黄斌. 98例肺结核患者IL-10和IL-6表达水平的临床研究[J].中外医疗,2013(3):27-28.
[12] Iyer SS,Cheng G. Role of Interleukin 10 transcriptional regulation in inflammation and autoimmune disease[J]. Crit Rev Immunol,2012,32(1):23-63.
[13]王晓义,谢松松,程丽,等.新疆维吾尔族和汉族肺结核患者白细胞介素-10及人肿瘤坏死因子-α表达水平的比较研究[J].中国临床药理学杂志,2017,33(22):2230-2232.
[14] Bartzeliotou AI,Margeli AP,Tsironi M. Circulating levels of adhesion molecules and markers of endothelial activation in acute inflammation induced by prolonged brisk exercise[J]. Clin Biochem,2007,40(11):765-770.
[15] Hoffmann SC,Stanley EM,Darrin Cox E,et al. Association of cytokine polymorphic inheritance and in vitro cytokine production in anti-CD3/CD28-stimulated peripheral blood lymphocytes[J].Transplantation,2001,72(8):1444-1450.
[16] Westendorp RG,Langermans JA,Huizinga TW,et al. Genetic influence on cytokine production and fatal meningococcal disease[J]. Lancet,1997,349(9046):170-173.
[17]朱雅婷,魏娟,张永军,等.新疆维吾尔族、汉族人群IL-10基因多态性与分泌量的相关性研究[J].中国免疫学杂志,2013,29(10):1022-1027.
[18] Gallagher PM,Lowe G,Fitzgerald T,et al. Association of IL-10polymorphism with severity of illness in community acquired pneumonia[J]. Thorax,2003,58(2):154-156.
[19] Sainz J,Hassan L,Perez E,et al. Interleukin-10 promoter polymorphism as risk factor to develop invasive pulmonary aspergillosis[J]. Immunol,2007,109(1):76-82.
[20] Reuss E,Fimmers R,Kruger A,et al. Differential regulation of interleukin-10 production by genetic and environmental factors-a twin study[J]. Genes Immun,2002,3(7):407-413.
[21]杜若甫.我国的人类群体遗传学研究[J].生物学通报,1997,32(7):9-12.
[22]艾琼华,肖辉,赵建新,等.维吾尔族的体质特征研究[J].人类学学报,1993,12(4):357-364.
[23]袁义达,杜若甫.中国十七个民族间的遗传距离的初步研究[J].遗传学报,1983,10(5):398-405.