甘草饮片及其四种炮制品的HPLC指纹图谱研究
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摘要
目的:利用高效液相色谱法建立了甘草生品及其炮制品的指纹图谱,为甘草及其不同炮制品的质量控制提供依据。方法:采用色谱柱Dikma Technologies-C_(18)(200 mm×4.6 mm,5μm),以乙腈-0.05%磷酸水溶液为流动相,梯度洗脱,切换检测波长,流速1.0 mL·min~(-1)。结果:建立了甘草及4种炮制品的指纹图谱,共标定了15个共有特征峰,指认了其中7个成分。结论:运用HPLC建立甘草饮片的指纹图谱,可为药材质量评价提供更为全面的信息。
Objective:To establish the HPLC specific chromatogram for the quality control of prepared slices of Licorice and its four processed products.Methods:The separation was performed on a Dikma Technologies-C_(18) ODS(200 mm×4.6 mm,5 μm) column,with a gradient elution composed of acetonitrile and 0.05% phosphate acid.The column flow rate was 1.0 mL·min~(-1),switching wavelength with the time.Results:The HPLC fingerprint of licorice and four processed products were established.The fifteen common peaks were calibrated and seven peaks were recognized.Conclusion:Using HPLC to establish the fingerprint of Licorice can provide more comprehensive information for the quality evaluation of medicinal materials.
Objective:To establish the HPLC specific chromatogram for the quality control of prepared slices of Licorice and its four processed products.Methods:The separation was performed on a Dikma Technologies-C_(18) ODS(200 mm×4.6 mm,5 μm) column,with a gradient elution composed of acetonitrile and 0.05% phosphate acid.The column flow rate was 1.0 mL·min~(-1),switching wavelength with the time.Results:The HPLC fingerprint of licorice and four processed products were established.The fifteen common peaks were calibrated and seven peaks were recognized.Conclusion:Using HPLC to establish the fingerprint of Licorice can provide more comprehensive information for the quality evaluation of medicinal materials.
引文
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[3] 王胜超,张振凌,于文娜,等.人中黄的HPLC指纹图谱及炮制机理初探[J].中药材,2017,40(5):1088.
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[8] 陈璞,赵华,贺雅琴,等.续断HPLC 特征图谱及3 种活性成分的含量测定[J].中药材,2017,40(6):1373-1376.
[9] 窦霞,杨锡仓,甄小龙,等.党参及其不同炮制品HPLC指纹图谱研究[J].中药材,2017,40(5):1095.
[10] 周萃,杨燕云,张振秋,等.切换波长HPLC法同时测定甘草及其炮制品中7个物质的含量[J].药物分析杂志,2011,31(11):2067.
[2] 高雪岩,王文全,魏胜利,等.甘草及其活性成分的药理活性研究进展[J].中国中药杂志,2009,34(21):2695.
[3] 王胜超,张振凌,于文娜,等.人中黄的HPLC指纹图谱及炮制机理初探[J].中药材,2017,40(5):1088.
[4] 孙国祥,王佳庆.HPLC数字化指纹图谱控制甘草质量[J].中南药学,2009,7(1):51.
[5] 濮润,王卫星,王京辉,等.甘草饮片的快速HPLC指纹图谱分析[J].中国中药杂志,2008,33(22):2651.
[6] 冉懋雄,郭建民.现代中药炮制册[M].北京:中国中医药出版社,2002:288.
[7] 贾天柱.中药炮制学[M].上海:上海科学技术出版社,2008:212-213.
[8] 陈璞,赵华,贺雅琴,等.续断HPLC 特征图谱及3 种活性成分的含量测定[J].中药材,2017,40(6):1373-1376.
[9] 窦霞,杨锡仓,甄小龙,等.党参及其不同炮制品HPLC指纹图谱研究[J].中药材,2017,40(5):1095.
[10] 周萃,杨燕云,张振秋,等.切换波长HPLC法同时测定甘草及其炮制品中7个物质的含量[J].药物分析杂志,2011,31(11):2067.