摘要
背景:光生物调节是一种新型治疗方案,LED红光能否影响牙源干细胞增殖,是探索颌面部再生治疗新方案的关键。目的:比较不同光照能量LED红光对人牙周膜干细胞与人根尖乳头干细胞增殖的影响。方法:分离培养、流式鉴定获取人牙周膜干细胞和人根尖乳头干细胞。MTT法检测不同光照能量0,1,3,5 J/cm~2对两种牙源性干细胞增殖的影响。结果与结论:①非光照下根尖乳头干细胞增殖速度较牙周膜干细胞更快,1,3,5 J/cm~2 LED红光均能促进两种牙源性干细胞增殖;②LED红光能促进牙周膜干细胞缓慢增殖期和对数生长期(1-7d)增殖,其中5J/cm~2对早期影响最明显,后期1 J/cm~2增殖作用最强;③LED红光对根尖乳头干细胞对数生长后期(5-7 d)影响较明显,3 J/cm~2LED红光的增殖作用于第7天达到峰值;④根据人牙周膜干细胞和人根尖乳头干细胞对LED红光的时效依赖及量效依赖性,选定特定的光照能量及时间,可快速有效地促进细胞增殖。
BACKGROUND: Photobiomodulation is a new treatment option. Whether red light-emitting diodes can affect the proliferation and regeneration of dental stem cells is the key to exploring new treatments for maxillofacial regeneration OBJECTIVE: To compare the effects of red light-emitting diodes with different light energies on the proliferation of human periodontal ligament stem cells and apical papillary stem cells.METHODS: Periodontal ligament stem cells and apical papillary stem cells were isolated and cultured by flow cytometry. Proliferation of these cells was detected using MTT assay after exposure to red light-emitting diodes at different light energies(0, 1, 3, and 5 J/cm2).RESULTS AND CONCLUSION: Apical papillary stem cells proliferated faster than periodontal ligament stem cells under non-lighting environment. Red light emitting diodes at 1, 3, and 5 J/cm~2 promoted the proliferation of two kinds of odontogenic stem cells. The red light-emitting diode promoted the proliferation of periodontal ligament stem cells at proliferative and logarithmic growth phase(1-7 days). And 5 J/cm~2 red light emitting diode had the most obvious effect in early stage, and 1 J/cm~2 had the strongest effect in the late stage. The red light-emitting diode had a significant effect on the logarithmic growth(5-7 days) of apical papilla stem cells, and under 3 J/cm~2 red light-emitting diode the proliferation peaked on the 7 th day. Therefore, the red light-emitting diode can promote the proliferation of human periodontal ligament stem cells and apical papillary stem cells in dose-and time-dependent manner. Exposure to red light-emitting diode at proper energy and time can promote cell proliferation quickly and effectively.
引文
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