邛海湿地天然生物膜对环境改变的响应
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摘要
通过交换邛海湿地的两个典型地点的天然基质,并对交换培养后基质上的生物膜的藻类群落结构、生物量、叶绿素、胞外酶活性、EPS多糖含量等指标进行分析,观察环境变化后生物膜结构功能随时间的改变情况。研究结果表明:生物膜在交换培养后,藻类结构、叶绿素浓度、胞外酶活性、多糖含量等指标均发生改变。随着交换时间增长,生物膜均发生了一定变化,其变化符合生物膜群落生长特性和生物膜对环境的适应性规律,综合反映出邛海湿地生物膜对环境改变有明显的响应。
The response of biofilm community to the environmental change was the foundation of ecological monitoring and evaluation. It had been the research focus of the water ecological restoration. This research selected two typical sites in Qionghai Lake wetland and exchanged the biofilm on natural substrate to compare the changes of the structure and function of biofilm after the exchange culture. The indexes of biofilm for analysis included algal structure, biomass, chlorophyll, extracellular enzyme activity and the content of polysaccharides. The results showed that part of biofilm indexes changed including algae structure, chlorophyll concentration, extracellular enzyme activity and the contents of polysaccharide. It could be concluded that biofilm changed with the switching time and the changes were regularity to the growing characteristics of biofilm and adaptability to the environment. Comprehensively, the biofilm in Qionghai Lake wetland has obvious response to the environmental change.
The response of biofilm community to the environmental change was the foundation of ecological monitoring and evaluation. It had been the research focus of the water ecological restoration. This research selected two typical sites in Qionghai Lake wetland and exchanged the biofilm on natural substrate to compare the changes of the structure and function of biofilm after the exchange culture. The indexes of biofilm for analysis included algal structure, biomass, chlorophyll, extracellular enzyme activity and the content of polysaccharides. The results showed that part of biofilm indexes changed including algae structure, chlorophyll concentration, extracellular enzyme activity and the contents of polysaccharide. It could be concluded that biofilm changed with the switching time and the changes were regularity to the growing characteristics of biofilm and adaptability to the environment. Comprehensively, the biofilm in Qionghai Lake wetland has obvious response to the environmental change.
引文
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[2]王雪梅.白洋淀流域复合污染的生物膜监测方法适用性分析[J].农业环境科学学报,2014(9).DOI:doi:10.11654/jaes.2014.09.018.
[3]刘丰.海河流域典型河口复合污染特征及天然生物膜的响应[D].北京:北京师范大学,2012.
[4]杨帆.自然水体生物膜上主要组分生长规律及吸附特性[D].长春:吉林大学,2005.
[5]胡鸿钧,魏印心.中国淡水藻类[M].上海:上海科学技术出版社,2006.
[6]张宇,董艳珍,杨红.四川邛海湖湿地浮游藻类现状调查[J].广东农业科学,2012,39(13):197-199.DOI:10.3969/j.issn.1004-874X.2012.13.065.
[7]S M,TT D,O H,et al.Cadmium toxicity and bioaccumulation in freshwater biofilms[J].Archives of Environmental Contamination&Toxicology,2008,54(2):173-186.
[8]H.G,M.P,S.S.effect of copper on algal communities from oligotrophic calcareous streams[J].Journal of Phycology,2002,38(2):241-248.
[9]Terra W R,Ferreira C,de Bianchi A G.Distribution of digestive enzymes among the endo-and ectoperitrophic spaces and midgut cells of R hynchosciara and its physiological significance[J].Journal of Insect Physiology,1979,25(6):487-494.
[10]Dubois M,et al.A colorimetric method for the determination of sugars[J].Nature,1951.doi:10.1038/168167a0:167.
[11]本书编委会.水和废水监测分析方法[M].北京:中国环境出版社,2002.
[12]马牧源,刘静玲,杨志峰.生物膜法应用于海河流域湿地生态系统健康评价展望[J].环境科学学报,2010,30(2):226-236.
[13]Denkhaus E,et al.Chemical and physical methods for characterization of biofilms[J].Microchimica Acta,2007,158(1):1-27.
[14]Sabater S,Guasch H,Ricart M,et al.Monitoring the effect of chemicals on biological communities.The biofilm as an interface[J].Analytical&Bioanalytical Chemistry,2007,387(4):1425-1434.
[15]刘俊琢,张成君.硅藻指数在水环境监测与评价中的应用[J].环境监测管理与技术,2009,21(5):12-16.DOI:10.3969/j.issn.1006-2009.2009.05.004.
[16]Bonet B,Corcoll N,Acuňa V,et al.Seasonal changes in antioxidant enzyme activities of freshwater biofilms in a metal polluted Mediterranean stream[J].Science of the Total Environment,2013(444):60-72.
[17]MS B,JA W,C F,et al.Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein.[J].Journal of Cell Biology,1996,134(4):1031-1049.
[18]H B,T K,K S,et al.Radical-scavenging effects of Aloe arborescens Miller on prevention of pancreatic islet B-cell destruction in rats[J].Journal of Ethnopharmacology,2003,89(1):37-45.