竹茹多糖体外调节肠道菌群的作用研究
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摘要
目的研究竹茹多糖体外调节人体肠道菌群的作用,为竹茹多糖作为肠道微生态调节剂的研发提供理论依据。方法通过体外模拟发酵体系考察竹茹多糖对人体肠道菌群的调节作用;采用16S rDNA高通量测序技术分析肠道菌群组成,采用GC-MS法检测菌群代谢产物(短链脂肪酸)。结果竹茹多糖能够显著上调拟杆菌属、普雷沃茨菌属、双歧杆菌属、乳杆菌属、副拟杆菌属、考拉杆菌属、氨基酸球菌属、布劳特氏菌、严格厌氧梭菌属_13、Erysipelatoclostridium属、链型杆菌属、Flavonifractor属和韦荣球菌属的相对丰度,并显著下调梭杆菌属、嗜胆菌属、脱硫弧菌属、另枝菌属、Butyricimonas属、Butyricicoccus属、Eisenbergiella属、Faecalitalea属、Lachnoclostridium属、Lachnospiraceae_UCG-004属、Lachnospiraceae_UCG-008属、Lachnospiraceae_uncultured属、罕见小球菌属和土孢杆属的相对丰度;同时,竹茹多糖能够显著促进人体肠道菌群代谢产生乙酸、丙酸和丁酸。结论竹茹多糖能够显著调节人体肠道菌群,并促进其代谢产生有益于机体健康的短链脂肪酸。[营养学报,2019,41(1):45-52]
Objective To investigate the in vitro fermentation behavior of polysaccharides from bamboo shavings(named BSP) by human gut microbiota. Methods An in vitro fermentation system was used to investigate the effect of BSP on human gut microbiota. High-throughput sequencing technology(16 S rDNA) was used to analyze the composition of gut microbiota; GC-MS was used to analyze short-chain fatty acid. Results BSP could significantly modulate gut microbiota composition via increasing the relative abundances of genera Bacteroides, Prevotella_7, Bifidobacterium, Lactobacillus,Parabacteroides,Phascolarctobacterium, Acidaminococcus, Blautia, Clostridium_sensu_ stricto_13, Erysipelatoclostridium,Catenibacterium, Flavonifractor and Veillonella and decreasing the relative abundances of genera Fusobacterium, Bilophila,Desulfovibrio, Alistipes, Butyricimonas, Butyricicoccus, Eisenbergiella, Faecalitalea, Lachnoclostridium, Lachnospiraceae_UCG-004, Lachnospiraceae_UCG-008, Lachnospiraceae_uncultured, Subdoligran-ulum and Terrisporobacter. Meanwhile,BSP could significantly enhance the production of acetic acid, propionic acid and butyric acid. Conclusion BSP could significantly modulate human gut microbiota in vitro, and significantly enhance its production of short-chain fatty acids.[ACTA NUTRIMENTA SINICA, 2019, 41(1):45-52]
Objective To investigate the in vitro fermentation behavior of polysaccharides from bamboo shavings(named BSP) by human gut microbiota. Methods An in vitro fermentation system was used to investigate the effect of BSP on human gut microbiota. High-throughput sequencing technology(16 S rDNA) was used to analyze the composition of gut microbiota; GC-MS was used to analyze short-chain fatty acid. Results BSP could significantly modulate gut microbiota composition via increasing the relative abundances of genera Bacteroides, Prevotella_7, Bifidobacterium, Lactobacillus,Parabacteroides,Phascolarctobacterium, Acidaminococcus, Blautia, Clostridium_sensu_ stricto_13, Erysipelatoclostridium,Catenibacterium, Flavonifractor and Veillonella and decreasing the relative abundances of genera Fusobacterium, Bilophila,Desulfovibrio, Alistipes, Butyricimonas, Butyricicoccus, Eisenbergiella, Faecalitalea, Lachnoclostridium, Lachnospiraceae_UCG-004, Lachnospiraceae_UCG-008, Lachnospiraceae_uncultured, Subdoligran-ulum and Terrisporobacter. Meanwhile,BSP could significantly enhance the production of acetic acid, propionic acid and butyric acid. Conclusion BSP could significantly modulate human gut microbiota in vitro, and significantly enhance its production of short-chain fatty acids.[ACTA NUTRIMENTA SINICA, 2019, 41(1):45-52]
引文
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[15]Han X,Song H,Wang Y,et al.Sodium butyrate protects the intestinal barrier function in peritonitic mice.[J].Int J Clin Exp Med,2015,8:4000-4007.
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[18]Dodd D,Mackie RI,Cann IKO.Xylan degradation,a metabolic property shared by rumen and human colonic Bacteroidetes[J].Mol Microbiol,2011,79:292-304.
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[20]Rogowski A,Briggs JA,Mortimer JC,et al.Glycan complexity dictates microbial resource allocation in the large intestine[J].Nat Commun,2016,6:7481.
[21]Ndeh D,Gilbert H J.Biochemistry of complex glycan depolymerisation by the human gut microbiota[J].Fems Microbiol Rev,2018,42:146-164.
[22]Noor S O,Ridgway K,Scovell L,et al.Ulcerative colitis and irritable bowel patients exhibit distinct abnormalities of the gut microbiota.[J].BMCGastroenterol,2010,10:134.
[23]Takahashi K,Nishida A,Fujimoto T,et al.Reduced abundance of butyrate-producing bacteria species in the fecal microbial community in Crohn's disease[J].Digestion,2016,93:59-65.
[24]Wu H,Esteve E,Tremaroli V,et al.Metformin alters the gut microbiome of individuals with treatment-naive type2 diabetes,contributing to the therapeutic effects of the drug[J].Nat Med,2017,23:850-858.
[25]Wu F,Guo X,Zhang J,et al.Phascolarctobacterium faecium abundant colonization in human gastrointestinal tract[J].Exp Ther Med,2017,14:3122-3126.
[26]Kosugi A,Murashima K,Doi R H.Xylanase and acetyl xylan esterase activities of XynA,a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation[J].Appl Environ Microb,2002,68:6399-6402.
[27]Wu Y,Wu J,Chen T,et al.Fusobacterium nucleatum potentiates intestinal tumorigenesis in mice via a Toll-Like receptor 4/p21-activated kinase 1 cascade[J].Digest Dis Sci,2018,63:1210-1218.
[28]Bahr SM,Weidemann BJ,Castro AN,et al.Risperidone-induced weight gain is mediated through shifts in the gut microbiome and suppression of energy expenditure[J].EBio Medicine,2015,2:1725-1734.
[29]Zhao L,Zhang Q,Ma W,et al.A combination of quercetin and resveratrol reduces obesity in high-fat diet-fed rats by modulation of gut microbiota.[J].Food Funct,2017,8:4644-4656.
[30]Zhang C,Zhang M,Pang X,et al.Structural resilience of the gut microbiota in adult mice under high-fat dietary perturbations.[J].ISME J,2012,6:1848-1857.
[31]Kameyama K,Itoh K.Intestinal colonization by a Lachnospiraceae bacterium contributes to the development of diabetes in obese mice[J].Microbes Environ,2014,29:427-430.
[32]Fu X,Cao C,Ren B,et al.Structural characterization and in vitro fermentation of a novel polysaccharide from Sargassum thunbergii and its impact on gut microbiota[J].Carbohydr Polym,2018,183:230-239.
[2]Shen R,Dang X,Dong J,et al.Effects of oatβ-glucan and barleyβ-glucan on fecal characteristics,intestinal microflora,and intestinal bacterial metabolites in rats[J].J Agric Food Chem,2012,60:11301-11308.
[3]Flint H J,Bayer E A,Rincon M T,et al.Polysaccharide utilization by gut bacteria:potential for new insights from genomic analysis[J].Nat Rev Microbiol,2008,6:121-131.
[4]DoréJ,Blottière H.The influence of diet on the gut microbiota and its consequences for health[J].Curr Opin Biotech,2015,32:195-199.
[5]Pastell H,Westermann P,Meyer AS,et al.In vitro fermentation of arabinoxylan-derived carbohydrates by bifidobacteria and mixed fecal microbiota[J].J Agric Food Chem,2009,57:8598-8606.
[6]黄菊青.竹茹多糖的化学结构和免疫活性研究[D].浙江大学,2015.
[7]Huang J,Pang M,Li G,et al.Alleviation of cyclophosphamide-induced immunosuppression in mice by naturally acetylated hemicellulose from bamboo shavings[J].Food Agric Immunol,2017,28:328-342.
[8]Minekus M,Alminger M,Alvito P,et al.A standardised static in vitro digestion method suitable for food-an international consensus[J].Food Funct,2014,5:1113-1124.
[9]Mandalari G,Nueno-Palop C,Bisignano G,et al.Potential prebiotic properties of almond(Amygdalus communis L.)seeds.[J].Appl Environ Microb,2008,74:4264-4270.
[10]García-Villalba R,Giménez-Bastida J A,García-Conesa MT,et al.Alternative method for gas chromatographymass spectrometry analysis of short-chain fatty acids in faecal samples.[J].J Sep Sci,2012,35:1906-1913.
[11]Zhou W,Yan Y,Mi J,et al.Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from bee collected pollen of Chinese wolfberry[J].J Agric Food Chem,2018,66:898-907.
[12]Den B G,Van E K,Groen A K,et al.The role of short-chain fatty acids in the interplay between diet,gut microbiota,and host energy metabolism.[J].J Lipid Res,2013,54:2325-2340.
[13]Kabat A M,Srinivasan N,Maloy KJ.Modulation of immune development and function by intestinal microbiota.[J].Trends Immunol,2014,35:507-517.
[14]Rios-Covian D,Ruas-Madiedo P,Margolles A,et al.Intestinal short chain fatty acids and their link with diet and human health[J].Front Microbiol,2016,7:185.
[15]Han X,Song H,Wang Y,et al.Sodium butyrate protects the intestinal barrier function in peritonitic mice.[J].Int J Clin Exp Med,2015,8:4000-4007.
[16]Davila A M,Blachier F,Gotteland M,et al.Intestinal luminal nitrogen metabolism:role of the gut microbiota and consequences for the host[J].Pharmacol Res,2013,69:114-126.
[17]Bourriaud C,Robins R J,Martin L,et al.Lactate is mainly fermented to butyrate by human intestinal microfloras but inter-individual variation is evident[J].J Appl Microbiol,2005,99:201-212.
[18]Dodd D,Mackie RI,Cann IKO.Xylan degradation,a metabolic property shared by rumen and human colonic Bacteroidetes[J].Mol Microbiol,2011,79:292-304.
[19]Dodd D,Moon Y H,Swaminathan K,et al.Transcriptomic analyses of xylan degradation by Prevotella bryantii and insights into energy acquisition by xylanolytic bacteroidetes[J].J Biol Chem,2010,285:30261-30273.
[20]Rogowski A,Briggs JA,Mortimer JC,et al.Glycan complexity dictates microbial resource allocation in the large intestine[J].Nat Commun,2016,6:7481.
[21]Ndeh D,Gilbert H J.Biochemistry of complex glycan depolymerisation by the human gut microbiota[J].Fems Microbiol Rev,2018,42:146-164.
[22]Noor S O,Ridgway K,Scovell L,et al.Ulcerative colitis and irritable bowel patients exhibit distinct abnormalities of the gut microbiota.[J].BMCGastroenterol,2010,10:134.
[23]Takahashi K,Nishida A,Fujimoto T,et al.Reduced abundance of butyrate-producing bacteria species in the fecal microbial community in Crohn's disease[J].Digestion,2016,93:59-65.
[24]Wu H,Esteve E,Tremaroli V,et al.Metformin alters the gut microbiome of individuals with treatment-naive type2 diabetes,contributing to the therapeutic effects of the drug[J].Nat Med,2017,23:850-858.
[25]Wu F,Guo X,Zhang J,et al.Phascolarctobacterium faecium abundant colonization in human gastrointestinal tract[J].Exp Ther Med,2017,14:3122-3126.
[26]Kosugi A,Murashima K,Doi R H.Xylanase and acetyl xylan esterase activities of XynA,a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation[J].Appl Environ Microb,2002,68:6399-6402.
[27]Wu Y,Wu J,Chen T,et al.Fusobacterium nucleatum potentiates intestinal tumorigenesis in mice via a Toll-Like receptor 4/p21-activated kinase 1 cascade[J].Digest Dis Sci,2018,63:1210-1218.
[28]Bahr SM,Weidemann BJ,Castro AN,et al.Risperidone-induced weight gain is mediated through shifts in the gut microbiome and suppression of energy expenditure[J].EBio Medicine,2015,2:1725-1734.
[29]Zhao L,Zhang Q,Ma W,et al.A combination of quercetin and resveratrol reduces obesity in high-fat diet-fed rats by modulation of gut microbiota.[J].Food Funct,2017,8:4644-4656.
[30]Zhang C,Zhang M,Pang X,et al.Structural resilience of the gut microbiota in adult mice under high-fat dietary perturbations.[J].ISME J,2012,6:1848-1857.
[31]Kameyama K,Itoh K.Intestinal colonization by a Lachnospiraceae bacterium contributes to the development of diabetes in obese mice[J].Microbes Environ,2014,29:427-430.
[32]Fu X,Cao C,Ren B,et al.Structural characterization and in vitro fermentation of a novel polysaccharide from Sargassum thunbergii and its impact on gut microbiota[J].Carbohydr Polym,2018,183:230-239.